Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 562199
Title Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems
Author(s) Suarez Ruiz, Catalina A.; Kwaijtaal, Jennifer; Peinado, Oriol Cabau; Berg, Corjan Van Den; Wijffels, Rene H.; Eppink, Michel H.M.
Source ACS sustainable chemistry & engineering 8 (2020)6. - ISSN 2168-0485 - p. 2441 - 2452.
Department(s) Bioprocess Engineering
FBEZ, Financiele Administratie
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Aqueous two-phase systems - Biomolecules - Cholinium-based ionic liquids - Downstream processes - Microalgae

We aim to develop liquid-liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.

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