Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 562402
Title Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve
Author(s) Bottacini, Francesca; Morrissey, Ruth; Roberts, Richard John; James, Kieran; Breen, Justin Van; Egan, Muireann; Lambert, Jolanda; Limpt, Kees Van; Knol, Jan; Connell Motherway, Mary O.; Sinderen, Douwe Van
Source Nucleic acids research 46 (2018)4. - ISSN 0305-1048 - p. 1860 - 1877.
DOI https://doi.org/10.1093/nar/gkx1289
Department(s) MolEco
VLAG
Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2018
Abstract

Bifidobacterium breve represents one of the most abundant bifidobacterial species in the gastrointestinal tract of breast-fed infants, where their presence is believed to exert beneficial effects. In the present study whole genome sequencing, employing the PacBio Single Molecule, Real-Time (SMRT) sequencing platform, combined with comparative genome analysis allowed the most extensive genetic investigation of this taxon. Our findings demonstrate that genes encoding Restriction/Modification (R/M) systems constitute a substantial part of the B. breve variable gene content (or variome). Using the methylome data generated by SMRT sequencing, combined with targeted Illumina bisulfite sequencing (BS-seq) and comparative genome analysis, we were able to detect methylation recognition motifs and assign these to identified B. breve R/M systems, where in several cases such assignments were confirmed by restriction analysis. Furthermore, we show that R/M systems typically impose a very significant barrier to genetic accessibility of B. breve strains, and that cloning of a methyltransferase-encoding gene may overcome such a barrier, thus allowing future functional investigations of members of this species.

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