Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 562413
Title Immunocapturing of extracellular vesicles on stainless steel for multi-modal individual characterization with correlative light, electron and probe microscopy
Author(s) Beekman, Pepijn; Martinez, Agustin Enciso; Terstappen, Leon; Otto, Cees; Gac, Séverine Le
Source In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - Chemical and Biological Microsystems Society (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ) - ISBN 9781510897571 - p. 2402 - 2403.
Event 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, 2018-11-11/2018-11-15
Department(s) Organic Chemistry
Publication type Contribution in proceedings
Publication year 2018
Keyword(s) AFM - Correlative light - Electron and probe microscopy - Extracellular vesicles - Raman - SEM
Abstract

Here, we report a robust platform for multi-modal analysis of immuno-captured individual extracellular vesicles (EVs). Stainless steel substrates were surface-modified to covalently immobilize specific antibodies targeting proteins found on EVs. Using PDMS microchannels, EVs were selectively captured on the substrates. Next, individual EVs were retraced and correlatively characterized here using SEM, AFM and Raman Spectroscopy.

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