|Title||Immunocapturing of extracellular vesicles on stainless steel for multi-modal individual characterization with correlative light, electron and probe microscopy|
|Author(s)||Beekman, Pepijn; Martinez, Agustin Enciso; Terstappen, Leon; Otto, Cees; Gac, Séverine Le|
|Source||In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - Chemical and Biological Microsystems Society (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ) - ISBN 9781510897571 - p. 2402 - 2403.|
|Event||22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, 2018-11-11/2018-11-15|
|Publication type||Contribution in proceedings|
|Keyword(s)||AFM - Correlative light - Electron and probe microscopy - Extracellular vesicles - Raman - SEM|
Here, we report a robust platform for multi-modal analysis of immuno-captured individual extracellular vesicles (EVs). Stainless steel substrates were surface-modified to covalently immobilize specific antibodies targeting proteins found on EVs. Using PDMS microchannels, EVs were selectively captured on the substrates. Next, individual EVs were retraced and correlatively characterized here using SEM, AFM and Raman Spectroscopy.