|Title||Micro magnetic resonance imaging of murine liver tissue slices on a microfluidic perfusion device|
|Author(s)||Sharma, Manvendra; Patra, Bishnubrata; Hale, William; Karsten, Ruby E.H.; Salentijn, Gert I.J.; Grajewski, Maciej; Fuhrer, Erwin; Zakhurdaeva, Anna; Mager, Dario; Korvink, Jan; Olinga, Peter; Verpoorte, Elisabeth; Utz, Marcel|
|Source||In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - Chemical and Biological Microsystems Society (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ) - ISBN 9781510897571 - p. 1722 - 1724.|
|Event||22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, 2018-11-11/2018-11-15|
|Publication type||Contribution in proceedings|
|Keyword(s)||Micro-MRI - NMR - PCLS|
Micro-MRI (Magnetic Resonance Imaging) and NMR (Nuclear Magnetic Resonance) spectroscopy are used to image and study metabolic activity of precision-cut liver slices (PCLS) on a microfluidic perfusion device. The imaging experiments were performed under static medium conditions while metabolic activity was monitored under continuous flow of medium. LDH (Lactate dehydrogenase) leakage assay was performed to assess the tissue viability on different days. MR images having ~30 (μm)2 in-plane resolution are recorded. This method could be used to culture and monitor PCLS non-invasively.