Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 562458
Title Profiling of MicroRNAs in the biofluids of livestock species
Author(s) Ioannidis, Jason; Risse, Judith; Donadeu, F.X.
Source In: Methods in Molecular Biology / Ying, S.Y., Humana Press Inc. (Methods in Molecular Biology ) - ISBN 9781493976003 - p. 65 - 77.
DOI https://doi.org/10.1007/978-1-4939-7601-0_5
Department(s) Bioinformatics
Publication type Peer reviewed book chapter
Publication year 2018
Keyword(s) Biomarker - Cow - Follicular fluid - microRNA - miRNA - Plasma - Sequencing
Abstract

The value of circulating microRNAs (miRNAs) as noninvasive biomarkers of human disease has been extensively demonstrated. Significant potential also exists in other species, particularly in relation to control of veterinary diseases and selection/monitoring of production traits in livestock. Although robust protocols have been developed for miRNA profiling of human biofluids, significant optimization may be required before these can be applied to other species. In this chapter, we describe protocols for small-RNA sequencing and RT-qPCR analyses of plasma samples from livestock species. In addition, we provide brief data analysis protocols for small-RNA sequencing and RT-qPCR data. Finally, we highlight important considerations for these protocols such as low RNA yield, platform-specific biases, and optimal normalization approaches.

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