Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 562462
Title Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol-Ene or Thiol-Yne Coupling Chemistry
Author(s) Bañuls, Maria Jose; Jiménez-Meneses, Pilar; Meyer, Albert; Vasseur, Jean Jacques; Morvan, François; Escorihuela, Jorge; Puchades, Rosa; Maquieira, Ángel
Source Bioconjugate Chemistry 28 (2017)2. - ISSN 1043-1802 - p. 496 - 506.
DOI https://doi.org/10.1021/acs.bioconjchem.6b00624
Department(s) Organic Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2017
Abstract

Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.

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