|Title||Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol-Ene or Thiol-Yne Coupling Chemistry|
|Author(s)||Bañuls, Maria Jose; Jiménez-Meneses, Pilar; Meyer, Albert; Vasseur, Jean Jacques; Morvan, François; Escorihuela, Jorge; Puchades, Rosa; Maquieira, Ángel|
|Source||Bioconjugate Chemistry 28 (2017)2. - ISSN 1043-1802 - p. 496 - 506.|
|Publication type||Refereed Article in a scientific journal|
Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.