Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 562507
Title Identificação e quantificação de genes diferencialmente expressos associados ao declínio dos citros (Citrus spp.)
Author(s) Renato Abreu, José de; Paiva, Luciano Vilela; Dita Rodríguez, M.A.; Silva, Anderson; Henriques, A.R.; Chalfun-Junior, A.
Source Ciencia e Agrotecnologia 39 (2015)1. - ISSN 1413-7054 - p. 32 - 38.
DOI https://doi.org/10.1590/S1413-70542015000100004
Department(s) Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Differential gene expression - RT-qPCR - Subtractive hybridization
Abstract

Brazil is the largest citrus producer in the world, being responsible for more than 20% of its production, which is, however still low due to phytosanitary issues such as citrus blight. Citrus blight is an anomaly whose causes still have not yet been determined, therefore there are no efficient control measures to minimize the production losses with the use of resistant varieties being considered the most appropriate method. However, little is known about the genes involved in the defense response of the plants to this anomaly. Considering that many physiological alterations associated with plant stress responses are controlled at a transcriptional level, in this study we sought the identification and characterization of the gene expression products differentially expressed in the response to the citrus blight. Through the suppressive subtractive hybridization technique, expressed cDNA libraries were built using mRNAs isolated from “Cravo” lemon tree roots (Citrus limonia L. Osbeck) under “Pera” orange (Citrus sinensis L. Osbeck) of healthy and sick plants. 129 clones were obtained by subtraction and their sequences were compared in databases. 34 of them linked to proteins associated to stress processes, while the others were similar to sequences of unknown functions or did not present similarity with sequences deposited in the databases. 3 genes were selected and their expressions were studied by RT - qPCR in real-time. Plants with citrus blight presented an increase of the expression level in two of those genes, suggesting that these can be directly involved with this anomaly.

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