Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 562982
Title Fish Macrophages Show Distinct Metabolic Signatures Upon Polarization
Author(s) Wentzel, Annelieke S.; Janssen, Joëlle J.E.; Boer, Vincent C.J. de; Veen, Wouter G. van; Forlenza, Maria; Wiegertjes, Geert F.
Source Frontiers in Immunology 11 (2020). - ISSN 1664-3224 - 1 p.
DOI https://doi.org/10.3389/fimmu.2020.00152
Department(s) Cell Biology and Immunology
Human and Animal Physiology
Experimental Zoology
Infection Biology
WIAS
Aquaculture and Fisheries
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) extracellular flux analysis - glycolysis - M1 M2 macrophage polarization - metabolic reprogramming - oxidative metabolism - oxidative phosphorylation (OXPHOS) - Seahorse - teleost
Abstract

Macrophages play important roles in conditions ranging from host immune defense to tissue regeneration and polarize their functional phenotype accordingly. Next to differences in the use of L-arginine and the production of different cytokines, inflammatory M1 macrophages and anti-inflammatory M2 macrophages are also metabolically distinct. In mammals, M1 macrophages show metabolic reprogramming toward glycolysis, while M2 macrophages rely on oxidative phosphorylation to generate energy. The presence of polarized functional immune phenotypes conserved from mammals to fish led us to hypothesize that a similar metabolic reprogramming in polarized macrophages exists in carp. We studied mitochondrial function of M1 and M2 carp macrophages under basal and stressed conditions to determine oxidative capacity by real-time measurements of oxygen consumption and glycolytic capacity by measuring lactate-based acidification. In M1 macrophages, we found increased nitric oxide production and irg1 expression in addition to altered oxidative phosphorylation and glycolysis. In M2 macrophages, we found increased arginase activity, and both oxidative phosphorylation and glycolysis were similar to control macrophages. These results indicate that M1 and M2 carp macrophages show distinct metabolic signatures and indicate that metabolic reprogramming may occur in carp M1 macrophages. This immunometabolic reprogramming likely supports the inflammatory phenotype of polarized macrophages in teleost fish such as carp, similar to what has been shown in mammals.

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