Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 563290
Title In vitro toxicological characterisation of the antifungal compound soybean toxin (SBTX)
Author(s) Arantes, Mariana Reis; Peijnenburg, Ad; Hendriksen, Peter J.M.; Stoopen, Geert; Almeida, Thiago Silva; Souza, Terezinha Maria; Farias, Davi Felipe; Carvalho, Ana Fontenele Urano; Rocha, Talita Magalhães; Leal, Luzia Kalyne Almeida Moreira; Vasconcelos, Ilka Maria; Oliveira, Jose Tadeu Abreu
Source Toxicology in Vitro 65 (2020). - ISSN 0887-2333
DOI https://doi.org/10.1016/j.tiv.2020.104824
Department(s) BU Toxicology, Novel Foods & Agrochains
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Antifungal agent - Cytotoxicity - SBTX - Toxicogenomics
Abstract

Soybean toxin (SBTX) is a protein isolated from soybean seeds and composed of two polypeptide subunits (17 and 27 kDa). SBTX has in vitro activity against phytopathogenic fungi such as Cercospora sojina, Aspergillus niger, and Penicillium herguei, and yeasts like Candida albicans, C. parapsilosis, Kluyveromyces marxiannus, and Pichia membranifaciens. The present study aimed to analyze in vitro whether SBTX causes any side effects on non-target bacterial and mammalian cells that could impede its potential use as a novel antifungal agent. SBTX at 100 μg/mL and 200 μg/mL did not hinder the growth of the bacteria Salmonella enterica (subspecies enterica serovar choleraesuis), Bacillus subtilis (subspecies spizizenii) and Staphylococcus aureus. Moreover, SBTX at concentrations up to 500 μg/mL did not significantly affect the viability of erythrocytes, neutrophils, and human intestinal Caco-2 cells. To study whether SBTX could induce relevant alterations in gene expression, in vitro DNA microarray experiments were conducted in which differentiated Caco-2 cells were exposed for 24 h to 100 μg/mL or 200 μg/mL SBTX. SBTX up-regulated genes involved in cell cycle and immune response pathways, but down-regulated genes that play a role in cholesterol biosynthesis and platelet degranulation pathways. Thus, although SBTX did not affect bacteria, nor induced cytotoxity in mammalian cells, it affected some biological pathways in the human Caco-2 cell line that warrants further investigation.

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