Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 563602
Title Integrated linkage map of haliotis midae linnaeus based on microsatellite and SNP markers
Author(s) Vervalle, Jessica; Hepple, Juli Ann; Jansen, Suzaan; Plessis, Jana Du; Wang, Peizheng; Rhode, Clint; Roodt-Wilding, Rouvay
Source Journal of Shellfish Research 32 (2013)1. - ISSN 0730-8000 - p. 89 - 103.
DOI https://doi.org/10.2983/035.032.0115
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) abalone - Haliotis midae - linkage map - microsatellites - single nucleotide polymorphisms
Abstract

The South African abalone Haliotis midae Linnaeus is the most important aquaculture species in South Africa. Marker-assisted selection is envisioned to play an integral part of the genetic improvement program of abalone, and therefore the generation of linkage maps for quantitative trait loci analyses are necessary. This study reports on a first-generation linkage map for H. midae based on microsatellite and single nucleotide polymorphism (SNP) markers. Ten full-sib families were screened with a total of 508 molecular markers derived from genomic and expressed sequence tag sequences. Linkage maps were constructed for each of the families and combined to create an integrated linkage map. The integrated linkage map consists of 186 markers that included 116 microsatellites and 70 SNPs. These markers mapped to 18 linkage groups, which corresponds to the haploid chromosome number of H. midae. The average genome length was estimated at 1,312 cM, displaying an average marker interval of 6.88 cM with 80% genome coverage. The female map is 1.16-fold longer than the male map, indicating differences in recombination rate between the sexes. The association of markers with known genes as well as with transposon elements was also investigated. This study resulted in the first linkage map constructed for any haliotid in which both microsatellite and SNP markers were used, and the results provide a framework for future applications in quantitative trait loci identification.

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