Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 564313
Title Metagenomic-and cultivation-based exploration of anaerobic chloroform biotransformation in hypersaline sediments as natural source of chloromethanes
Author(s) Peng, Peng; Lu, Yue; Bosma, Tom N.P.; Nijenhuis, Ivonne; Nijsse, Bart; Shetty, Sudarshan A.; Ruecker, Alexander; Umanets, Alexander; Ramiro-Garcia, Javier; Kappler, Andreas; Sipkema, Detmer; Smidt, Hauke; Atashgahi, Siavash
Source Microorganisms 8 (2020)5. - ISSN 2076-2607
DOI https://doi.org/10.3390/microorganisms8050665
Department(s) MolEco
Microbiology
Systems and Synthetic Biology
WIMEK
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Biotransformation - Chloroform - Hypersaline lakes - Metagenome
Abstract

Chloroform (CF) is an environmental contaminant that can be naturally formed in various environments ranging from forest soils to salt lakes. Here we investigated CF removal potential in sediments obtained from hypersaline lakes in Western Australia. Reductive dechlorination of CF to dichloromethane (DCM) was observed in enrichment cultures derived from sediments of Lake Strawbridge, which has been reported as a natural source of CF. No CF removal was observed in abiotic control cultures without artificial electron donors, indicating biotic CF dechlorination in the enrichment cultures. Increasing vitamin B12 concentration from 0.04 to 4 µM in enrichment cultures enhanced CF removal and reduced DCM formation. In cultures amended with 4 µM vitamin B12 and13C labelled CF, formation of13CO2 was detected. Known organohalide-respiring bacteria and reductive dehalogenase genes were neither detected using quantitative PCR nor metagenomic analysis of the enrichment cultures. Rather, members of the order Clostridiales, known to co-metabolically transform CF to DCM and CO2, were detected. Accordingly, metagenome-assembled genomes of Clostridiales encoded enzymatic repertoires for the Wood-Ljungdahl pathway and cobalamin biosynthesis, which are known to be involved in fortuitous and nonspecific CF transformation. This study indicates that hypersaline lake microbiomes may act as a filter to reduce CF emission to the atmosphere.

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