Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 564659
Title Identification and functional characterization of the Arabidopsis Snf1-related protein kinase SnRK2.4 phosphatidic acid-binding domain
Author(s) Julkowska, Magdalena M.; McLoughlin, Fionn; Galvan-Ampudia, Carlos S.; Rankenberg, Johanna M.; Kawa, Dorota; Klimecka, Maria; Haring, Michel A.; Munnik, Teun; Kooijman, Edgar E.; Testerink, Christa
Source Plant, Cell & Environment 38 (2015)3. - ISSN 0140-7791 - p. 614 - 624.
DOI https://doi.org/10.1111/pce.12421
Department(s) Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) phosphatidic acid - phospholipid binding - root system architecture - SnRK2.10
Abstract

Phosphatidic acid (PA) is an important signalling lipid involved in various stress-induced signalling cascades. Two SnRK2 protein kinases (SnRK2.4 and SnRK2.10), previously identified as PA-binding proteins, are shown here to prefer binding to PA over other anionic phospholipids and to associate with cellular membranes in response to salt stress in Arabidopsis roots. A 42 amino acid sequence was identified as the primary PA-binding domain (PABD) of SnRK2.4. Unlike the full-length SnRK2.4, neither the PABD-YFP fusion protein nor the SnRK2.10 re-localized into punctate structures upon salt stress treatment, showing that additional domains of the SnRK2.4 protein are required for its re-localization during salt stress. Within the PABD, five basic amino acids, conserved in class 1 SnRK2s, were found to be necessary for PA binding. Remarkably, plants overexpressing the PABD, but not a non-PA-binding mutant version, showed a severe reduction in root growth. Together, this study biochemically characterizes the PA-SnRK2.4 interaction and shows that functionality of the SnRK2.4 PABD affects root development.

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