Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 565679
Title Methanol based chain elongation with acetate to n-butyrate and isobutyrate at varying selectivities dependent on pH
Author(s) Leeuw, Kasper de; Smit, Sanne de; Oossanen, Sabine van; Moerland, Merijn; Buisman, Cees; Strik, David
Source Wageningen University & Research
Department(s) Biological Recovery & Re-use Technology
Bioprocess Engineering
WIMEK
Publication type Dataset
Publication year 2020
Keyword(s) PRJEB36205 - ERP119366 - metagenome
Abstract Biomass fermentation technologies offer alternative methods to produce platform chemicals that currently originate from fossil sources. This research showed that an enriched microbiome was capable to produce iso-butyric acid via methanol based chain elongation of acetate. A long term continuous reactor experiment showed that the selectivity for isobutyrate (i-C4) and/or n-butyrate (n-C4) could be reversibly adjusted by changing the reactor pH. A reactor pH of 6.75 led to formation of (carbon per total carbon of products) of 0.78 n-C4 and 0.024 i-C4, whereas a reactor pH of 5.2 led to a selectivity of 0.25 n-C4 and 0.63 i-C4 . This shift in product spectrum was also represented by a shift in microbial composition. The results suggest that an Eubacterium genus is responsible for the formation of n-C4, whereas a Clostridium luticellarii strain is responsible for the formation of a mixture of i-C4 and n¬¬-C4. The formation of n and i-C4 at a low pH was observed to be coupled according to the thermodynamics of isomerization. At a reactor pH of 5.5 and 5.2 the product ratio of i-C4:n-C4 approached the ratio of 0.684 i-C4 : 0.316 n-C4, which is the theoretical ratio that would be achieved when i-C4 and n-C4 would be formed at amounts determined by the equilibrium of isomerization. Various batch experiments at pH 5.5 and 5.2 confirmed that addition of either n-C4 or i-C4 at the start of the batch would immediately lead to the formation of the other butyrate component. Moreover, batch experiments performed at pH 6.5 immediately produced mainly n-C4 and led to the development of a completely different microbiome. The imposed pH as a strong selective pressure can immediately facilitate changes in product selectivities for n-C4 and i-C4 during methanol based chain elongation of acetate.
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