Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 565864
Title Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
Author(s) Bastiaan-Net, Shanna; Pina-Pérez, Maria C.; Dekkers, Bas J.W.; Westphal, Adrie H.; America, Antoine H.P.; Ariëns, Renata M.C.; Jong, Nicolette W. de; Wichers, Harry J.; Mes, Jurriaan J.
Source Protein Science 29 (2020)7. - ISSN 0961-8368 - p. 1581 - 1595.
DOI https://doi.org/10.1002/pro.3856
Department(s) Food, Health & Consumer Research
VLAG
EPS
Laboratory of Plant Physiology
Biochemistry
BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) Anacardium occidentale - Bet v 1-like - cashew nut - in silico allergenicity analysis - oral allergy syndrome (OAS) - PR10 - RNA-seq
Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.

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