Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 65125
Title Tandem mass spectrometric analysis of Aspergillus niger pectin methylesterase: mode of action on fully methylesterified oligogalacturonates
Author(s) Kester, H.C.M.; Esteban Warren, M.; Orlando, R.; Benen, J.A.E.; Bergmann, C.; Visser, J.
Source Journal of Biochemistry 346 (2000). - ISSN 0021-924X - p. 469 - 474.
Department(s) Molecular Genetics of Industrial Micro-organisms
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract The substrate specificity and the mode of action of Aspergillus niger pectin methylesterase (PME) was determined using both fully methyl-esterified oligogalacturonates with degrees of polymerization (DP) 2–6 and chemically synthesized monomethyl trigalacturonates. The enzymic activity on the different substrates and a preliminary characterization of the reaction products were performed by using high-performance anion-exchange chromatography at neutral pH. Electrospray ionization tandem MS (ESI–MS/MS) was used to localize the methyl esters on the 18O-labelled reaction products during the course of the enzymic reaction. A. niger PME is able to hydrolyse the methyl esters of fully methyl-esterified oligogalacturonates with DP 2, and preferentially hydrolyses the methyl esters located on the internal galacturonate residues, followed by hydrolysis of the methyl esters towards the reducing end. This PME is unable to hydrolyse the methyl ester of the galacturonate moiety at the non-reducing end.
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