Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 66859
Title CD Spectroscopy of proteins adsorbed at flat hydrophilic quartz and hydrophobic Teflon surfaces
Author(s) Vermeer, A.W.P.; Norde, W.
Source Journal of Colloid and Interface Science 225 (2000). - ISSN 0021-9797 - p. 394 - 397.
DOI https://doi.org/10.1006/jcis.2000.6769
Department(s) Physical Chemistry and Colloid Science
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract Spectroscopic methods provide a powerful tool for studying the properties of proteins at interfaces. The protein accumulated in one adsorbed layer is frequently less than the minimum mass of protein required by a detection method. In such a case (as is the case in circular dichroism spectroscopy) the sorbent material is usually supplied as dispersion. However, light scattering by the dispersed particles often interferes with the measurement of the circular dichroism of the protein. Therefore, there is a strong need for an experimental setup that enables these measurements to be made using flat surfaces. An example of such a setup is the multiplate quartz cell presented here. The potential of this multiplate quartz cell is shown by some preliminary circular dichroism measurements of IgG adsorbed on different types of surfaces.
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