Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 9329
Title Towards a rapid and reliable detection method for iris severe mosaic virus in iris bulbs.
Author(s) Vlugt, C.I.M. van der; Derks, A.F.L.M.; Dijkstra, J.; Goldbach, R.W.
Source Acta Horticulturae 234 (1988). - ISSN 0567-7572 - p. 191 - 199.
Department(s) Laboratory of Virology
Publication type Refereed Article in a scientific journal
Publication year 1988
Keyword(s) plantenziekten - plantenvirussen - potyvirus - opslag - plant diseases - plant viruses - storage
Categories Plant and Crop Protection (General)
Abstract Detection of iris severe mosaic virus (ISMV) in ISMV-infected iris bulbs during storage, by either ELISA or electron microscopy has been problematic. We have applied different storage-temperature treatments and a cutting method as possible procedures to enhance ISMV detection in stored iris bulbs cv. Professor Blaauw. While at lifting ISMV could not be detected in the bulbs, gradually the virus became detectable in time when bulbs were stored at about 17°C. Better results were obtained when the cutting method was employed. Using ELISA, a 100 ␜core was obtained for infected bulbs that were cut 1 month prior to testing. The virus could be detected only in tissue adjacent to the cut surface. These results offer good prospects for the development of a reliable detection method for ISMV in iris bulbs.
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