Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 343384
Title Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.)
Author(s) Supena, E.D.J.; Suharsono, S.; Jacobsen, E.; Custers, J.B.M.
Source Plant Cell Reports 25 (2006)1. - ISSN 0721-7714 - p. 1 - 10.
DOI https://doi.org/10.1007/s00299-005-0028-y
Department(s) Plant Breeding
PRI Bioscience
EPS-4
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) direct somatic embryogenesis - anther-culture - plant-regeneration - different genotypes - brassica-napus - androgenesis - invitro - tobacco - lines - resistance
Abstract Various systems of anther and microspore cultures were studied to establish an efficient doubled haploid production method for Indonesian hot pepper (Capsicum annuum L.). A shed-microspore culture protocol was developed which outperformed all the previously reported methods of haploid production in pepper. The critical factors of the protocol are: selection of flower buds with more than 50% late unicellular microspores, a 1 day 4°C pretreatment of the buds, followed by culture of the anthers in double-layer medium system for 1 week at 9°C and thereafter at 28°C in continuous darkness. The medium contained Nitsch components and 2% maltose, with 1% activated charcoal in the solid under layer and 2.5 ¿M zeatin and 5 ¿M indole-3-acetic acid in the liquid upper layer. All the ten genotypes of hot pepper tested, responded to this protocol. The best genotypes produced four to seven plants per original flower bud. This protocol can be used as a potential tool for producing doubled haploid plants for hot pepper breeding.
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