Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 560052
Title Revisiting the Role of Master Regulators in Tomato Ripening
Author(s) Wang, Rufang; Angenent, Gerco C.; Seymour, Graham; Maagd, Ruud A. de
Source Trends in Plant Science 25 (2020)3. - ISSN 1360-1385 - p. 291 - 301.
DOI https://doi.org/10.1016/j.tplants.2019.11.005
Department(s) Laboratory of Molecular Biology
EPS
BIOS Plant Development Systems
Publication type Refereed Article in a scientific journal
Publication year 2020
Keyword(s) CRISPR- mutagenesis - gain-of-function - mutants - ripening - tomato - transcription factors
Abstract

The study of transcriptional regulation of tomato ripening has been led by spontaneous mutations in transcription factor (TF) genes that completely inhibit normal ripening, suggesting that they are ‘master regulators’. Studies using CRISPR/Cas9 mutagenesis to produce knockouts of the underlying genes indicate a different picture, suggesting that the regulation is more robust than previously thought. This requires us to revisit our model of the regulation of ripening and replace it with one involving a network of partially redundant components. At the same time, the fast rise of CRISPR/Cas mutagenesis, resulting in unexpectedly weak phenotypes, compared with knockdown technology, suggests that compensatory mechanisms may obscure protein functions. This emphasises the need for assessment of these mechanisms in plants and for the careful design of mutagenesis experiments.

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