Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Effects of the pure flavonoids epicatechin and quercetin on vascular function and cardiometabolic health: a randomized double-blind, placebo-controlled, crossover trial
Dower, J.I. ; Geleijnse, J.M. ; Gijsbers, L. ; Zock, P.L. ; Kromhout, D. ; Hollman, P.C.H. - \ 2015
American Journal of Clinical Nutrition 101 (2015)5. - ISSN 0002-9165 - p. 914 - 921.
homeostasis model assessment - reduces blood-pressure - flavanol-rich cocoa - insulin-resistance - dark chocolate - cardiovascular-disease - hypertensive subjects - endothelial function - plasma epicatechin - catechin contents
BACKGROUND: Prospective cohort studies showed inverse associations between the intake of flavonoid-rich foods (cocoa and tea) and cardiovascular disease (CVD). Intervention studies showed protective effects on intermediate markers of CVD. This may be due to the protective effects of the flavonoids epicatechin (in cocoa and tea) and quercetin (in tea). OBJECTIVE: We investigated the effects of supplementation of pure epicatechin and quercetin on vascular function and cardiometabolic health. DESIGN: Thirty-seven apparently healthy men and women aged 40-80 y with a systolic blood pressure (BP) between 125 and 160 mm Hg at screening were enrolled in a randomized, double-blind, placebo-controlled, crossover trial. CVD risk factors were measured before and after 4 wk of daily flavonoid supplementation. Participants received (-)-epicatechin (100 mg/d), quercetin-3-glucoside (160 mg/d), or placebo capsules for 4 wk in random order. The primary outcome was the change in flow-mediated dilation from pre- to postintervention. Secondary outcomes included other markers of CVD risk and vascular function. RESULTS: Epicatechin supplementation did not change flow-mediated dilation significantly (1.1% absolute; 95% CI: -0.1%, 2.3%; P = 0.07). Epicatechin supplementation improved fasting plasma insulin (¿ insulin: -1.46 mU/L; 95% CI: -2.74, -0.18 mU/L; P = 0.03) and insulin resistance (¿ homeostasis model assessment of insulin resistance: -0.38; 95% CI: -0.74, -0.01; P = 0.04) and had no effect on fasting plasma glucose. Epicatechin did not change BP (office BP and 24-h ambulatory BP), arterial stiffness, nitric oxide, endothelin 1, or blood lipid profile. Quercetin-3-glucoside supplementation had no effect on flow-mediated dilation, insulin resistance, or other CVD risk factors. CONCLUSIONS: Our results suggest that epicatechin may in part contribute to the cardioprotective effects of cocoa and tea by improving insulin resistance. It is unlikely that quercetin plays an important role in the cardioprotective effects of tea. This study was registered at clinicaltrials.gov as NCT01691404.
Isoflavone supplement composition and equol producer status affect gene expression in adipose tissue: a double-blind, randomized, placebo-controlled crossover trial in postmenopausal women
Velpen, V. van der; Geelen, A. ; Hollman, P.C.H. ; Schouten, E.G. ; Veer, P. van 't; Afman, L.A. - \ 2014
American Journal of Clinical Nutrition 100 (2014)5. - ISSN 0002-9165 - p. 1269 - 1277.
in-vivo - glucose-metabolism - lipid-metabolism - soy isoflavones - phytoestrogens - profiles - protein - macrophage - insights - genotype
Background: Isoflavone supplements, consumed by women experiencing menopausal symptoms, are suggested to have positive effects on menopause-related adiposity and cardiovascular disease risk profile, but discussions about their safety are still ongoing. Objective: The objective was to study the effects of an 8-wk consumption of 2 different isoflavone supplements compared with placebo on whole-genome gene expression in the adipose tissue of postmenopausal women. Design: This double-blind, randomized, placebo-controlled crossover intervention consisted of 2 substudies, one with a low-genistein (LG) supplement (56% daidzein + daidzin, 16% genistein + genistin, and 28% glycitein + glycitin) and the other with a high-genistein (HG) supplement (49% daidzein + daidzin, 41% genistein + genistin, and 10% glycitein + glycitin). Both supplements provided ~100 mg isoflavones/d (aglycone equivalents). After the 8-wk isoflavone and placebo period, whole-genome arrays were performed in subcutaneous adipose tissue of postmenopausal women (n = 26 after LG, n = 31 after HG). Participants were randomized by equol-producing phenotype, and data analysis was performed per substudy for equol producers and nonproducers separately. Results: Gene set enrichment analysis showed downregulation of expression of energy metabolism–related genes after LG supplementation (n = 24) in both equol-producing phenotypes and oppositely regulated expression for equol producers (down) and nonproducers (up) after HG supplementation (n = 31). Expression of inflammation-related genes was upregulated in equol producers but downregulated in nonproducers, independent of supplement type. Only 4.4–7.0% of the genes with significantly changed expression were estrogen responsive. Body weight, adipocyte size, and plasma lipid profile were not affected by isoflavone supplementation. Conclusions: Effects of isoflavones on adipose tissue gene expression were influenced by supplement composition and equol-producing phenotype, whereas estrogen-responsive effects were lacking. LG isoflavone supplementation resulted in a caloric restriction–like gene expression profile for both producer phenotypes and pointed toward a potential beneficial effect, whereas both supplements induced anti-inflammatory gene expression in equol producers.
Consumption of both low and high (-)-epicatechin apple puree attenuates platelet reactivity and increases plasma concentrations of nitric oxide metabolites: A randomized controlled trial
Gasper, A. ; Hollands, W. ; Casgrain, A. ; Saha, S. ; Teucher, B. ; Dainty, J.R. ; Venema, D.P. ; Hollman, P.C.H. - \ 2014
Archives of Biochemistry and Biophysics 559 (2014). - ISSN 0003-9861 - p. 29 - 37.
flavanol-rich cocoa - reduces blood-pressure - green tea catechins - black tea - grape juice - endothelial dysfunction - regular ingestion - food sources - red wine - in-vivo
We hypothesised that consumption of flavanol-containing apple puree would modulate platelet activity and increase nitric oxide metabolite status, and that high flavanol apple puree would exert a greater effect than low flavanol apple puree. 25 subjects consumed 230 g of apple puree containing 25 and 100 mg epicatechin (low and high flavanol apple puree, respectively) and aspirin (75 mg) in random order. Measurements were made at baseline, acutely after treatment (2, 6 and 24 h), and after 14 d of treatment. Low flavanol apple puree significantly attenuated ADP and epinephrine-induced integrin-ß3 expression 2 h and 6 h after consumption and ADP and epinephrine-induced P-selectin expression within 2 h of consumption. High flavanol apple puree attenuated epinephrine and ADP-induced integrin-ß3 expression after 2 and 6 h. ADP and epinephrine-induced integrin-ß3 expression was significantly attenuated 2, 6 and 24 h after consumption of aspirin, whilst 14 d aspirin consumption attenuated collagen-induced P-selectin expression only. The plasma total nitric oxide metabolite conc. was significantly increased 6 h after consumption of both low and high flavanol apple purees. In conclusion, consumption of apple purees containing ¿25 or 100 mg flavanols transiently attenuated ex vivo integrin-ß3 and P-selectin expression and increased plasma nitric oxide metabolite conc. in healthy subjects, but the effect was not enhanced for the high flavanol apple puree.
Large inter-individual variation in isoflavone plasma concentration limits use of isoflavone intake data for risk assessement
Velpen, V. van der; Hollman, P.C.H. ; Nielen, M. van; Schouten, E.G. ; Mensink, M.R. ; Veer, P. van 't; Geelen, A. - \ 2014
European Journal of Clinical Nutrition 68 (2014). - ISSN 0954-3007 - p. 1141 - 1147.
randomized controlled-trials - soy isoflavones - premenopausal women - soybean isoflavones - equol production - gut microflora - healthy-adults - habitual diet - bioavailability - humans
Background/objectives: Isoflavones are present in soy foods and soy-based supplements. Despite low plasma isoflavone concentrations in the general Western population, concentrations in supplement users exceed those suggested to be beneficial for health in Asian populations, raising concerns for adverse effects. To aid risk assessment, quantification of the relation between isoflavone intake and plasma concentrations is essential. Subjects/methods: Plasma samples were collected from postmenopausal women in three placebo-controlled crossover studies with 8-week periods for supplements (two studies, ~100¿mg isoflavones/day, n=88) or 4-week periods for soy foods (one study, ~48¿mg isoflavones/day, n=15). Plasma isoflavone concentrations (daidzein, equol, genistein and glycitein) were quantified using high-performance liquid chromatography and electrochemical detection. The association between plasma concentrations and isoflavone intake, equol producer status, intake–producer interaction and background dietary intake was assessed based on the assumption of a log-linear relation. Results: Median plasma total isoflavone concentrations after the soy food and supplement interventions were respectively 2.16 and 3.47¿µmol/l for equol producers and 1.30 and 2.39¿µmol/l for non-producers. Regression analysis showed that doubling isoflavone intake increased plasma concentrations by 55–62% (±s.e. 1–2%, R2>0.87) for daidzein, genistein, equol (only for producers) and total isoflavones; for glycitein the association was weaker (15±1%, R2=0.48). Adjustments for energy, carbohydrate and fat intake did not affect these estimates. Inter-individual variation, estimated based on repeated measures in one of the studies, was 30–96%. Conclusions: Although the relation between isoflavone intake and plasma concentrations was adequately quantified, the use of isoflavone intake data for risk assessment needs caution due to large inter-individual variation in plasma concentrations.
Unravelling of the health effects of polyphenols is a complex puzzle complicated by metabolism
Hollman, P.C.H. - \ 2014
Archives of Biochemistry and Biophysics 559 (2014)2014. - ISSN 0003-9861 - p. 100 - 105.
cardiovascular-disease mortality - flavonoid intake - blood-pressure - prospective cohort - adhesion molecule - vascular function - heart-disease - cancer-risk - diet - consumption
Plant metabolism creates complex mixtures of polyphenols in plant foods. Epidemiology and human trials reduced this complexity, by studying specific foods; subclasses of polyphenols; individual polyphenols, or total antioxidant capacity (TAC). This implies the following assumptions: (1) a limited number of potent polyphenols exists; (2) well-defined natural potent mixtures of polyphenols exist; (3) polyphenols share a common biological activity (e.g. antioxidant activity). To find potent polyphenols (1st assumption), in vitro screening has been widely applied, but most published results are of limited use because metabolism, changing biological activity profoundly, has frequently not been considered. The abundant anecdotal evidence for natural potent mixtures of polyphenols (2nd assumption) on the internet is very hard to verify. Additionally, cross-cultural studies have revealed the potency of e.g. cocoa. Polyphenols share the antioxidant phenolic group which inspired researchers to measure their antioxidant activity, thus greatly reducing complexity (3rd assumption). Unfortunately, the elegant antioxidant hypothesis has to be rejected, because poor absorption and extensive metabolism annihilate any contribution to the endogenous body antioxidants. In conclusion, the above assumptions are hard to verify, and no quick answers are to be expected. Future research should focus on structure–activity relations at nanomolar levels and explore metabolomics.
Quercetin decreases high-fat diet induced body weight gain and accumulation of hepatic and circulating lipids in mice
Hoek-van den Hil, E.F. ; Schothorst, E.M. van; Stelt, I. van der; Swarts, J.J.M. ; Venema, D.P. ; Sailer, M. ; Vervoort, J.J.M. ; Hollman, P.C.H. ; Rietjens, I. ; Keijer, J. - \ 2014
Genes & Nutrition 9 (2014). - ISSN 1555-8932 - 8 p.
cardiovascular-disease - gene-expression - c57bl/6j mice - acid - risk - hepatocytes - metabolism - flavonoids - obesity - women
Dietary flavonoids may protect against cardiovascular diseases (CVD). Increased circulating lipid levels and hepatic lipid accumulation are known risk factors for CVD. The aim of this study was to investigate the effects and underlying molecular mechanisms of the flavonoid quercetin on hepatic lipid metabolism in mice with high-fat diet induced body weight gain and hepatic lipid accumulation. Adult male mice received a 40 energy% high-fat diet without or with supplementation of 0.33 % (w/w) quercetin for 12 weeks. Body weight gain was 29 % lower in quercetin fed mice (p <0.01), while the energy intake was not significantly different. Quercetin supplementation lowered hepatic lipid accumulation to 29 % of the amount present in the control mice (p <0.01). 1H nuclear magnetic resonance serum lipid profiling revealed that the supplementation significantly lowered serum lipid levels. Global gene expression profiling of liver showed that cytochrome P450 2b (Cyp2b) genes, key target genes of the transcription factor constitutive androstane receptor (Car; official symbol Nr1i3), were downregulated. Quercetin decreased high-fat diet induced body weight gain, hepatic lipid accumulation and serum lipid levels. This was accompanied by regulation of cytochrome P450 2b genes in liver, which are possibly under transcriptional control of CAR. The quercetin effects are likely dependent on the fat content of the diet.
Direct comparison of health effects by dietary polyphenols at equimolar doses in wildtype moderate high-fat fed C57BL/6JOlaHsd mice
Schothorst, E.M. van; Bunschoten, A. ; Hoevenaars, F.P.M. ; Stelt, I. van der; Janovska, P. ; Venema, D.P. ; Kopecky, J. ; Hollman, P.C.H. ; Keijer, J. - \ 2014
Food Research International 65 (2014)Part A. - ISSN 0963-9969 - p. 95 - 102.
adipose-tissue - body-weight - induced obesity - disease risk - quercetin - bioavailability - expression - flavonoids - leptin - (-)-epigallocatechin-3-gallate
Polyphenols generally show beneficial health effects upon supplementation in diet-induced obese rodent models, including reduced body weight gain and reduced levels of markers for cardiovascular diseases (CVD). However, there appear to be large differences between studies, which might be due to differences in models, strains, dietary background, or even concentration of polyphenol that is used. Therefore, we performed a systematic phenotypic evaluation of the effects of selected polyphenols in wildtype C57BL/6JOlaHsd mice. Epigallocatechin-gallate, quercetin, and resveratrol, representing three different phenolic classes, were each added in equimolar amounts (0.50% (w/w), 0.33%, and 0.25%, respectively) to a purified moderate high fat (30energy%) diet for 12 weeks. We studied the polyphenol-induced physiological and molecular effects between them and relative to the nonsupplemented control group during and at the end of the nutritional intervention. Results showed that these polyphenols were present in circulation, but did not induce beneficial health effects as analysed by oral glucose tolerance testing or serum adipokines and CVD-markers such as vascular adhesion molecules. Remarkably, transcriptomics of white adipose tissue showed overlapping sets of significantly differential transcript levels between these polyphenols; AMPK and Notch signalling were affected by these polyphenols. However, mitochondrial processes and mitochondrial density in this tissue did not differ between the polyphenols, which suggested that there was no direct effect on adipose tissue.
Quercetin induces hepatic lipid omega-oxidation and lowers serum lipid levels in mice [Mus Musculus]
Schothorst, E.M. van; Keijer, J. ; Bunschoten, J.E. ; Hil, E.F. van den; Rietjens, I.M.C.M. ; Hollman, P.C.H. - \ 2013
human nutrition and health - GSE39140 - PRJNA170118
Elevated circulating lipid levels are known risk factors for cardiovascular diseases (CVD). In order to examine the effects of quercetin on hepatic lipid metabolism and detailed serum lipid profiles, mice received a mild-high-fat diet without (control) or with supplementation of 0.33% (w/w) quercetin for 12 weeks. Gas chromatography and 1H nuclear magnetic resonance were used to measure quantitatively serum lipid profiles and whole genome microarray analysis was used to identify the responsible mechanisms in liver. There were no significant differences found in mean body weight, energy intake and hepatic lipid accumulation between the quercetin and control group. In serum of quercetin-fed mice, TG levels were decreased with 15%, poly unsaturated fatty acids (PUFA) were increased with 14% and saturated fatty acids were decreased. Palmitic acid, oleic acid, and linoleic acid were all decreased in quercetin-fed mice by 9-15%. Both palmitic acid and oleic acid can be oxidized by omega-oxidation. Indeed, gene expression profiling showed that quercetin increased hepatic lipid metabolism, especially omega-oxidation. At the gene level, this was reflected by the up regulation of cytochrome P450 (Cyp) 4a10, Cyp4a14, Cyp4a31 and Acyl-CoA thioesterase 3 (Acot3). Two relevant regulators, Cytochrome P450 oxidoreductase (Por, rate limiting for cytochrome P450s) and the transcription factor Constitutive androstane receptor (Car; official symbol Nr1i3) were also up regulated in the quercetin-fed mice. We conclude that quercetin intake increased hepatic lipid omega-oxidation and lowered corresponding circulating lipid levels, a process that may involve Por and Car, and results in a potential beneficial CVD preventive effect.
Quercetin induces hepatic lipid omega-oxidation and lowers serum lipid levels in mice
Hoek-van den Hil, E.F. ; Keijer, J. ; Bunschoten, A. ; Vervoort, J. ; Stankova, B. ; Bekkenkamp, M. ; Herreman, L. ; Venema, D.P. ; Hollman, P.C.H. ; Tvrzicka, E. ; Rietjens, I. ; Schothorst, E.M. van - \ 2013
Quercetin induces hepatic lipid omega-oxidation and lowers serum lipid levels in mice
Hoek-van den Hil, E.F. ; Keijer, J. ; Bunschoten, A. ; Vervoort, J.J.M. ; Stankova, B. ; Bekkenkamp, M. ; Herreman, L. ; Venema, D.P. ; Hollman, P.C.H. ; Tvrzicka, E. ; Rietjens, I. ; Schothorst, E.M. van - \ 2013
In: Annals of Nutrition & Metabolism : Abstracts of the 20th International Congress of Nutrition. - Munich : Karger - ISBN 9783318025163 - p. 149 - 149.
A foresight study on emerging technologies: State of the art of omics technologies and potential applications. REPORT 1: Review on the state of art of omics technologies in risk assessment related to food and feed safety
Pielaat, A. ; Barker, A. ; Hendriksen, P.J.M. ; Hollman, P.C.H. ; Peijnenburg, A.A.C.M. ; Kuijle, B.H. ter - \ 2013
Parma (I) : EFSA (supporting publications EN-495) - 126 p.
Polyphenols and Cardiovascular Diseases: Is there a Role for ther Antioxidant Porperties?
Hollman, P.C.H. - \ 2013
Annals of Nutrition & Metabolism 62 (2013)2. - ISSN 0250-6807 - p. 149 - 149.
The potential health effects of antioxidants have attracted much interest and enthusiasm of consumers and food industry. Recently, we performed a Google search with the search term “antioxidant foods” and found more than 12 million hits! Among others, the internet gives lists of the “top 10 of antioxidant foods”, and heavily advertises “super antioxidant foods” etc. In this context, the large family of polyphenols, antioxidants ubiquitous in plant foods, has been studied extensively. Many authors have linked the antioxidant activity of polyphenols to the beneficial effects of vegetables and fruit. A still growing number of epidemiological studies showed that the intake of polyphenol-rich foods (tea, red wine, cacao, onions, and apples) was inversely associated with CVD [1]. These associations have also been demonstrated for individual polyphenols (mainly flavonols and flavan-3-ols) [2–4]. In addition, well conducted clinical trials with polyphenol-rich foods showed beneficial effects on intermediate markers of CVD [5, 6]. These studies might suggest that a relation exists between the antioxidant activity of polyphenols and their health effects. However, what’s the evidence for such a relation? The Total Antioxidant Capacity (TAC) of foods and plasma or serum has been commonly measured. These methods try to measure the total amount of antioxidants in these samples. Furthermore, products of lipid peroxidation, e.g. F2 isoprostanen, hydroperoxides and oxidised LDL have been frequently measured [7]. However, a causal relation between these biomarkers of antioxidant activity and CVD has not been established. Thus the physiological relevance of a potential change in this biomarkers is unclear [7]. So, there seems to be no beneficial direct antioxidant effect of polyphenols. The reasons for this lack of effect will be discussed. References: 1 Arts IC, Hollman PC: Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr 2005;81:317S–325S. 2 Hertog MGL, Feskens EJ M, Hollman PCH, Katan MB, Kromhout D: Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 1993;342:1007–1011. 3 Arts IC, Hollman PC, Feskens EJ, Bueno de Mesquita HB, Kromhout D: Catechin intake might explain the inverse relation between tea consumption and ischemic heart disease: the Zutphen Elderly Study. Am J Clin Nutr 2001;74:227–232. 4 Hollman PC, Geelen A, Kromhout D: Dietary Flavonol Intake May Lower Stroke Risk in Men and Women. J Nutr 2010;140:600–604. 5 Hooper L, Kay C, Abdelhamid A, Kroon PA, Cohn JS, Rimm EB, Cassidy A: Effects of chocolate, cocoa, and flavan-3-ols on cardiovascular health: a systematic review and meta-analysis of randomized trials. Am J Clin Nutr 2012;95:740–751. 6 Ried K, Sullivan TR, Fakler P, Frank OR, Stocks NP: Effect of cocoa on blood pressure. Cochrane DB Syst Rev 2012:1–81.
Estrogen Receptor-Mediated Effects of Isoflavone Supplementation Were Not Observed in Whole-Genome Gene Expression Profiles of Peripheral Blood Mononuclear Cells in Postmenopausal, Equol-Producing Women
Velpen, V. van der; Geelen, A. ; Schouten, E.G. ; Hollman, P.C.H. ; Afman, L.A. ; Veer, P. van 't - \ 2013
The Journal of Nutrition 143 (2013)6. - ISSN 0022-3166 - p. 774 - 780.
oxidative-phosphorylation - inflammatory markers - dietary isoflavones - in-vitro - soy - cancer - health - mechanisms - phytoestrogens - consumption
Isoflavones (genistein, daidzein, and glycitein) are suggested to have benefits as well as risks for human health. Approximately one-third of the Western population is able to metabolize daidzein into the more potent metabolite equol. Having little endogenous estradiol, equol-producing postmenopausal women who use isoflavone supplements to relieve their menopausal symptoms could potentially be at high risk of adverse effects of isoflavone supplementation. The current trial aimed to study the effects of intake of an isoflavone supplement rich in daidzein compared with placebo on whole-genome gene expression profiles of peripheral blood mononuclear cells (PBMCs) in equol-producing, postmenopausal women. Thirty participants received an isoflavone supplement or a placebo for 8 wk each in a double-blind, randomized cross-over design. The isoflavone supplement was rich in daidzein (60%) and provided 94 mg isoflavones (aglycone equivalents) daily. Gene expression in PBMCs was significantly changed (P <0.05) in 357 genes after the isoflavone intervention compared with placebo. Gene set enrichment analysis revealed downregulated clusters of gene sets involved in inflammation, oxidative phosphorylation, and cell cycle. The expression of estrogen receptor (ER) target genes and gene sets related to ER signaling were not significantly altered, which may be explained by the low ERa and ERß expression in PBMCs. The observed downregulated gene sets point toward potential beneficial effects of isoflavone supplementation with respect to prevention of cancer and cardiovascular disease. However, whether ER-related effects of isoflavones are beneficial or harmful should be studied in tissues that express ERs
Microplastics in aquatic food chain : sources, measurement, occurrence and potential health risks
Hollman, P.C.H. ; Bouwmeester, H. ; Peters, R.J.B. - \ 2013
Wageningen : Rikilt - Institute of Food Safety (Report / RIKILT, Institute of Food Safety 2013.003) - 27
microplastics - ecotoxicologie - gezondheid - voedselketens - aquatische ecologie - ecotoxicology - health - food chains - aquatic ecology
Pollution of the environment with plastics is a growing problem, and is expected to persist for hundreds to thousands of years. As a result microplastics, plastic particles with size smaller than 5 mm, are ubiquitously present in the aquatic food chain. The present literature review shows that the information needed for such an evaluation is not available yet
Quercetin induces hepatic lipid omega-oxidation and lowers serum lipid levels in mice
Hoek-van den Hil, E.F. ; Keijer, J. ; Bunschoten, A. ; Vervoort, Jacques ; Stankova, B. ; Bekkenkamp-Grovestein, M. ; Herreman, L. ; Venema, D.P. ; Hollman, P.C.H. ; Tvrzicka, E. ; Rietjens, I. ; Schothorst, E.M. van - \ 2013
PLoS ONE 8 (2013)1. - ISSN 1932-6203 - 10 p.
fatty-acid oxidation - cytochrome-p450 reductase - liver-disease - receptor car - metabolism - dietary - rat - flavonoids - risk - hydroxylases
Elevated circulating lipid levels are known risk factors for cardiovascular diseases (CVD). In order to examine the effects of quercetin on lipid metabolism, mice received a mild-high-fat diet without (control) or with supplementation of 0.33% (w/w) quercetin for 12 weeks. Gas chromatography and 1H nuclear magnetic resonance were used to quantitatively measure serum lipid profiles. Whole genome microarray analysis of liver tissue was used to identify possible mechanisms underlying altered circulating lipid levels. Body weight, energy intake and hepatic lipid accumulation did not differ significantly between the quercetin and the control group. In serum of quercetin-fed mice, triglycerides (TG) were decreased with 14% (p
Proctection by Flavonal-Rich foods against vascular dysfunction and oxidative damage: 27th Hohenheim consensus conference
Sies, H. ; Hollman, P.C.H. ; Grune, T. ; Stahl, W. - \ 2012
Advances in Nutrition 3 (2012). - ISSN 2161-8313 - p. 217 - 221.
antioxidant capacity assays - arterial stiffness - protein oxidation - dietary-intake - health claims - comet assay - polyphenols - disease - flavonoids - consumption
Criteria for assessing the purported protection by flavanol-rich foods against vascular dysfunction and oxidative damage to biomolecules was the subject of the 27th Hohenheim Consensus Conference held on July 11, 2011. State-of-the-art evidence was put into perspective, focusing on several questions that were followed by a consensus answer. Among the topics addressed were the major sources of flavanols in the human diet, the bioavailability of flavanols, biomarkers for “health benefit,” and the biological function of flavanols. Consensus was reached on these topics. No conclusion was reached on the design of randomized, controlled trials for substantiation of health claims for flavanol-rich foods as to the necessity of a study arm with an isolated pharmacologically active compound, e.g., (-)-epicatechin.
Correction for measurement errors in an FFQ by using duplicate portion, 24 hour recall and a biomarker as the reference methods
Trijsburg, L.E. ; Vries, J.H.M. de; Geelen, A. ; Hollman, P.C.H. ; Veer, P. van 't; Hulshof, P.J.M. - \ 2012
Bioavailability of isoflavones in humans
Cassidy, A. ; Penalvo, J. ; Hollman, P.C.H. - \ 2012
In: Flavonoids and related compounds : bioavailability and function / Spencer, J.P.E., Crozier, A., CRC Press (Oxidative Stress and Disease ) - ISBN 9781439848265 - 471 p.
Nutrient analysis explained for non-chemists by using interactive e-learning material
Busstra, M.C. ; Hulshof, P.J.M. ; Houwen, J. ; Elburg, L. ; Hollman, P.C.H. - \ 2012
Journal of Food Composition and Analysis 25 (2012)1. - ISSN 0889-1575 - p. 88 - 95.
instructional-design - quality
The diverse educational and professional background of individuals involved in food composition data work presents challenges in their training. In particular, it is difficult to explain chemical analysis of nutrients to individuals lacking a background in chemistry. Therefore an interactive e-learning module entitled “Nutrient Analysis for Non-chemists” was developed. Interactive e-learning provides a powerful set of tools to stimulate a learning process tailored to the needs and background of course members. In its design, specific aims derived from theories on learning and instruction were first formulated: motivate the student; provide an authentic learning context; visualize important concepts; promote active learning; and avoid unnecessary cognitive load. The e-learning module developed contains a large variety of interactive exercises, animations, and background information. The following four topics or cases were elaborated: Fats and Fatty Acids, Proteins and Amino Acids, Carbohydrates and Fiber, and Elements. In diverse educational settings, the module was evaluated by course members of postgraduate courses who highly appreciated it with an overall score of 4.5 on a 5-point scale. The e-learning module that was developed can be nicely integrated into a blended learning course on food composition data. However, it is also very well suited for individual distance learning
Distribution, Elimination and Toxicity of Silver Nanoparticles and Silver Ions in Rats after 28-day Oral Exposure
Zande, M. van der; Vandebriel, R.J. ; Doren, E. ; Kramer, E.H.M. ; Herrera-Rivera, Z. ; serrano-Rojero, C.S. ; Gremmer, E.R. ; Mast, J. ; Peters, R.J.B. ; Hollman, P.C.H. ; Hendriksen, P.J.M. ; Marvin, H.J.P. ; Peijnenburg, A.A.C.M. ; Bouwmeester, H. - \ 2012
ACS Nano 6 (2012)8. - ISSN 1936-0851 - p. 7427 - 7442.
sprague-dawley rats - brain-barrier permeability - engineered nanoparticles - inhalation toxicity - tissue distribution - release - translocation - localization - deposition - kinetics
We report the results of a 28-day oral exposure study in rats, exposed to
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