Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Transfer of natural auto-antibodies via egg yolk in chickens divergently selected for natural antibodies binding keyhole limpet hemocyanin
Dijk, Kaylee S.E. van; Parmentier, Henk K. - \ 2020
Developmental and Comparative Immunology 102 (2020). - ISSN 0145-305X
Chicken - Maternal transfer - Natural (auto-) antibodies - Selection lines - Western blot

Barcodes of natural auto-antibody (NAAb) profiles based on staining intensity of isotypes binding numbers of self-(tissue) antigen fragments were suggested as parameters for immune diversity, and related to genetic background and health status in man, rodents and poultry. Here, hens, eggs and hatchlings from chicken lines divergently selected and bred for high (H line) or low (L line) total natural antibodies (NAb) levels in plasma binding keyhole limpet hemocyanin (KLH) at 16 weeks of age were tested for their NAAb repertoire binding chicken liver homogenate (CLH) fragments using quantitative Western immunoblotting. The aims of this study were 1. to detect line differences between the H and L line adult hens, eggs and hatchlings for the IgM and IgG isotypes binding CLH fragments, 2. study the presence of NAAb of both isotypes in yolk and albumen, as well as in hatchlings to detect a maternal NAAb transfer route via the egg to the hatchling, and 3. study whether new self-antigen binding isotypes and idiotypes are present in the hatchling. NAAb binding CLH fragments were found in plasma of adult hens (both IgM and IgG), in yolk (IgG only), and hatchlings (mostly IgG, but low levels of IgM). Auto-profiles of IgM showed homogeneity, while IgG profiles were heterogenic between individual hens and individual hatchlings. Significant higher levels as indicated by staining intensity and number of stained CLH fragments were found in plasma of hens genetically selected for high levels of NAb binding KLH. Lines could be clustered based on their auto-profiles indicating that profiles of self-binding IgM and IgG antibodies are genetically based. Visual comparison, clustering and correlation of hens and their hatchlings showed similarities for the IgG, but not the IgM isotype, indicating maternal transfer of IgG NAAb via the yolk. The IgM profile in the hatchlings on the other hand might represent neonatal self-binding antibody formation. As a consequence, hatchlings initially depend for self-binding antibodies on maternal IgG provision during early life.

Relationship of Free Fatty Acid and Natural Autoantibodies 2 Weeks Postpartum
Mayasari, N. ; Chen, J. ; Kemp, B. ; Parmentier, H. ; Knegsel, A. Van - \ 2019
In: The 3rd Sustainable Agriculture And Food Security (3rdICSAFS). - IOP Publishing (IOP Conference Series: Earth and Environmental Science 1)
Natural autoantibodies (NAAb) have been found in plasma and milk of dairy cows. Natural autoantibodies maintain homeostasis and may prevent infections. The objective of this study was to evaluate the relationship between concentration of plasma free fatty acid (FFA) and the levels of NAAb in plasma binding glutamate dehydrogenase (GD) and carbonic anhydrase (CA) in cows 2 weeks after calving. In total, 55 Holstein-Friesian dairy with 60-d dry period lengths and fed 2 early lactation diets (glucogenic or lipogenic). Blood was sampled at week 1 and 2 after calving. Result showed that from week 1 and 2 relative to calving, levels of IgG and IgM binding CA were positively related with concentration of FFA in plasma. Levels of IgM binding GD were negatively related with concentration of FFA in plasma. It is concluded that IgG and IgM binding CA in plasma might be an indicator for energy status and reflect metabolic status in dairy cows 2 weeks after calving.
Improving disease resistance in chickens: divergent selection on natural antibodies
Berghof, T.V.L. ; Poel, J.J. van der; Arts, J.A.J. ; Bovenhuis, H. ; Visker, M.H.P.W. ; Parmentier, H.K. - \ 2019
- p. 507 - 507.
Natural antibodies (NAb) are antibodies recognizing antigens without previous exposure to this antigen. Keyhole limpet hemocyanin (KLH)-binding NAb titers in chickens are heritable, and higher KLH-binding NAb titers have been associated with higher survival. This suggests that breeding for higher NAb titers might improve general disease resistance. A purebred White Leghorn chicken line was divergently selected and bred on total KLH-binding NAb titers at 16 weeks of age for 6 generations, and resulted in a High and Low line. The average estimated breeding value differences in KLH-binding NAb titers increased with 0.36 for total, 0.40 for IgM, and 0.32 for IgG per generation. Generations 4 and 6 of the selection lines were inoculated with an avian pathogenic Escherichia coli (APEC) at 8 days of age. Mortality and morbidity after 1 week were signicantly reduced in the High line compared to the Low line, which suggests a higher APEC resistance in the High line compared to the Low line. To investigate possible correlated responses on the immune system, several traits were measured at different ages in several generations: the High line showed higher different NAb titers at different ages, antibody concentrations, percentage of antibody-producing B cells, and bursa weight at young age compared to the Low line. This suggests that KLH-binding NAb selection has a favourable correlated response on the humoral adaptive immune system. No line differences were observed for T cells, γδ T cells, natural killer (NK) cells, and antigen-presenting cells (APC). This might indicate that the selection had no unfavourable correlated responses on other parts of the immune system. This selection experiment shows that selective breeding on total KLH-binding NAb titers at 16 weeks of age is possible, and that selection for higher NAb has a benecial effect on resistance to APEC infection. In addition, the selection experiment suggests a promising opportunity for improving general disease resistance without unfavourable correlated selection responses.
Natural antibody levels predict genetically-determined livability after intratracheal pathogenic Escherichia coli inoculation in chickens
Matthijs, M.G.R. ; Dwars, R.M. ; Berghof, Tom ; Parmentier, Henk - \ 2019
Powerpoint presentation, pagina OM-6
Genome-wide association study identifies loci influencing natural antibody titers in milk of Dutch Holstein-Friesian cattle
Cordero-Solorzano, Juan ; Parmentier, Henk K. ; Arts, Joop A.J. ; Poel, Jan van der; Koning, Dirk Jan de; Bovenhuis, Henk - \ 2019
Journal of Dairy Science 102 (2019)12. - ISSN 0022-0302 - p. 11092 - 11103.
dairy cattle - genome-wide association study - immunoglobulin - natural antibody

Natural antibodies (NAb) are produced without any antigenic stimulation as a part of the innate immune system and provide a first line of defense against pathogens. Hence, they may be a useful trait when estimating an animal's potential immune competence and in selection for disease resistance. The aim of this study was to identify genomic regions associated with different NAb traits in milk and potentially describe candidate genes. Milk samples from 1,695 first-lactation Holstein Friesian cows with titer measurements for keyhole limpet hemocyanin, lipopolysaccharide, lipoteichoic acid, and peptidoglycan-binding total NAb and isotypes IgG1, IgM, and IgA were used. Genome-wide association study analyses were performed using imputed 777K SNP genotypes, accounting for relationships using pedigree information. Functional enrichment analysis was performed on the significantly associated genomic regions to look for candidate genes. For IgM NAb, significant associations (false discovery rate <0.05) were found on Bos taurus autosome (BTA) 17, 18, and 21 with candidate genes related to immunoglobulin structure and early B cell development. For IgG1, associations were found on BTA3, and we confirmed a quantitative trait loci on BTA21 previously reported for IgG NAb in serum. Our results provide new insights into the regulation of milk NAb that will help unravel the complex relationship between milk immunoglobulins and disease resistance in dairy cattle.

Relationship between inflammatory biomarkers and oxidative stress with uterine health in dairy cows with different dry period lengths
Mayasari, Novi ; Trevisi, Erminio ; Ferrari, Annarita ; Kemp, Bas ; Parmentier, Henk K. ; Knegsel, Ariette T.M. Van - \ 2019
Translational Animal Science 3 (2019)2. - ISSN 2573-2102 - p. 607 - 619.
Cattle - Continuous milking - Inflammation - Oxidative stress - Uterine health

Earlier studies indicated that the inflammatory status of dairy cows in early lactation could not be fully explained by the negative energy balance (NEB) at that moment. The objective of the present study was to determine relationships between inflammatory biomarkers and oxidative stress with uterine health in dairy cows after different dry period lengths. Holstein-Friesian dairy cows were assigned to one of three dry period lengths (0-, 30-, or 60-d) and one of two early lactation rations (gluco-genic or lipogenic ration). Cows were fed either a glucogenic or lipogenic ration from 10-d before the expected calving date. Part of the cows which were planned for a 0-d dry period dried themselves off and were attributed to a new group (0 → 30-d dry period), which resulted in total in four dry period groups. Blood was collected (N = 110 cows) in weeks -3, -2, -1, 1, 2, and 4 relative to calving to determine bio-markers for inflammation, liver function, and oxidative stress. Uterine health status (UHS) was monitored by scoring vaginal discharge (VD) based on a 4-point scoring system (0, 1, 2, or 3) in weeks 2 and 3 after calving. Cows were classified as having a healthy uterine environment (HU, VD score = 0 or 1 in both weeks 2 and 3), nonrecovering uterine environment (NRU, VD score = 2 or 3 in week 3), or a recovering uterine environment (RU, VD score = 2 or 3 in week 2 and VD score= 0 or 1 in week 3). Independent of dry period length, cows with NRU had higher plasma haptoglobin (P = 0.05) and lower paraoxonase levels (P < 0.01) in the first 4 weeks after calving and lower liver functionality index (P < 0.01) compared with cows with HU. Cows with NRU had lower plasma albumin (P = 0.02) and creatinine (P = 0.02) compared with cows with a RU, but not compared with cows with HU. Independent of UHS, cows with a 0 → 30-d dry period had higher bil-irubin levels compared with cows with 0-, 30-, or 60-d dry period (P < 0.01). Cows with RU and fed a lipogenic ration had higher levels of albumin in plasma compared with cows with NRU and fed a lipogenic ration (P < 0.01). In conclusion, uterine health was related to biomarkers for inflammation (haptoglobin and albumin) and paraoxonase in dairy cows in early lactation. Cows which were planned for a 0-d dry period, but dried themselves off (0 → 30-d dry period group) had higher bilirubin levels, which was possibly related to a more severe NEB in these cows. Inflammatory biomarkers in dairy cows in early lactation were related to uterine health in this period.

Maternal Transfer of Natural (Auto-) Antibodies in Chickens
Rifqi Ismiraj, M. ; Arts, J.A.J. ; Parmentier, H.K. - \ 2019
Poultry Science 98 (2019)6. - ISSN 0032-5791 - p. 2380 - 2391.
natural (auto-) antibodies - divergent selection - maternal transfer
The presence and relative levels (titers) of IgM and IgG natural antibodies (NAb) binding keyhole limpet hemocyanin (KLH), and natural (auto-) antibodies (N(A)Ab) binding salmon double-stranded DNA (dsDNA), (oxidated-) phosphatidyl (phosphoryl) choline-conjugated bovine serum albumin (PC-BSA), PC-conjugated ovalbumin (PC-OVA), and OVA, respectively, were studied in adult hen plasma, egg yolk, egg albumen, plasma of their hatchlings, and in 8-day-old chick plasma. Birds and eggs were from 2 lines divergently selected for high or low NAb levels binding KLH. This study aimed to determine 1) correlated phenotypic responses of selection for NAb to KLH, 2) transfer of maternal NAb and N(A)Ab via egg compartments, 3) levels of likely maternal NAb and N(A)Ab in hatchlings and 8-day-old chicks, and 4) whether a composite trait: IgM anti-PC-BSA/IgG anti-dsDNA ratio in the compartments could be used as a parameter for health or immune status.

NAb and N(A)Ab to all tested antigens were found in adult hens, but low or no levels were found for IgM in yolk and IgG in albumen. Depending on the antigen, NAb and N(A)Ab were found in hatchlings and day 8 birds. Divergent selection and breeding based on NAb binding KLH affected antibody titers of almost all antigens in almost all compartments, in a similar way. Maternal transfer of NAb and N(A)Ab from the adult hen to offspring was via specific routes for specific antigens and isotypes, especially for IgG as suggested by cluster analyses and significant correlations. There was little indication of production of new NAb and N(A)Ab to the studied antigens in either the egg compartments or the hatchlings. A composite trait of IgM PC-BSA/IgG dsDNA ratio was as yet not indicative for immune status, as no significant differences were found between the lines for all compartments.

In conclusion, hens provide neonatal chickens with natural (self-) binding IgG antibodies that have been proposed to perform homeostatic functions during the period in which neonates do not produce these antibodies themselves.
Selective breeding for high natural antibody level increases resistance to avian pathogenic Escherichia coli (APEC) in chickens
Berghof, T.V.L. ; Matthijs, M.G.R. ; Arts, J.A.J. ; Bovenhuis, H. ; Dwars, R.M. ; Poel, J.J. van der; Visker, M.H.P.W. ; Parmentier, H.K. - \ 2019
Developmental and Comparative Immunology 93 (2019). - ISSN 0145-305X - p. 45 - 57.
APEC - Breeding - Chicken - Disease resistance - Escherichia coli - Natural antibody

Keyhole limpet hemocyanin (KLH)-binding natural antibody (NAb) titers in chickens are heritable, and higher levels have previously been associated with a higher survival. This suggests that selective breeding for higher NAb levels might increase survival by means of improved general disease resistance. Chickens were divergently selected and bred for total NAb levels binding KLH at 16 weeks of age for six generations, resulting in a High NAb selection line and a Low NAb selection line. To for test differences in disease resistance, chickens were challenged with avian pathogenic Escherichia coli (APEC) in two separate experiments. Chickens at 8 days of age received one of four intratracheal inoculations of 0.2 mL phosphate buffered saline (PBS): 1) mock inoculate, 2) with 0.2 mL PBS containing 108.20 colony-forming units (CFU)/mL APEC, 3) with 0.2 mL PBS containing 106.64 CFU/mL APEC, and 4) with 0.2 mL PBS containing 107.55 CFU/mL APEC. Mortality was recorded during 7 days post inoculation. Overall, 50–60% reduced mortality was observed in the High line compared to the Low line for all APEC doses. In addition, morbidity was determined of the surviving chickens at 15 days of age. The High line had lower morbidity scores compared to the Low line. We conclude that selective breeding for high KLH-binding NAb levels at 16 weeks of age increase APEC resistance in early life. This study and previous studies support the hypothesis that KLH-binding NAb might be used as an indicator trait for to selective breed for general disease resistance in an antigen non-specific fashion.

Selective breeding on natural antibodies in chickens: selection response, and correlated responses
Berghof, T.V.L. ; Poel, J.J. van der; Arts, J.A.J. ; Bovenhuis, H. ; Visker, M.H.P.W. ; Parmentier, H.K. - \ 2018
Effects of (a switch in) enriched vs barren housing on the response to reward loss in pigs in a negative contrast test
Luo, Lu ; Reimert, I. ; Smeets, Sharine ; Haas, E.N. de; Parmentier, H.K. ; Kemp, B. ; Bolhuis, J.E. - \ 2018
In: Proceedings of the 52nd Congress of the International Society for Applied Ethology. - Wageningen, The Netherlands : Wageningen Academic Publishers - ISBN 9789086863228 - p. 233 - 233.
Several studies suggest that animals in a negative emotional state are more sensitive to reward losses as shown by behavioural and neurophysiological responses. In a successive negative contrast (SNC) test, reward losses are induced by decreasing the size of the reward for a task for which animals have been trained. is SNC paradigm has not been widely used in pigs. It is well known that environmental enrichment positively inuences the welfare of pigs, and may induce a more optimistic emotional state, which could reduce their sensitivity to reward losses. We studied pigs in barren (B) or enriched (E) housing, experiencing either a switch in housing conditions at 7 weeks of age or not (4 treatment groups: EE, EB, BE, BB, n=8 pens per group) in an SNC runway task. We hypothesized that B housed pigs, particularly those that changed from E to B housing, would show an enhanced sensitivity to reward losses. One pig per pen was trained to run a 24.6 m U-shaped runway for 6 pieces and one for 1 piece of apple. Each pig received 3 trials per day, with a maximum of 120 sec/trial. Latency to start eating the reward was recorded, and the average was calculated per day. Aer 11 days, all pigs received 1 piece of apple only for another 11 days (reward shi: 6-1 vs 1-1 reward group), i.e. the group originally receiving 6 pieces of apple experienced a reward loss. Eects of pre-housing, post-housing, (original) reward size, day and interactions were analysed using mixed models with a random eect of animal. Fiy-one pigs were successfully trained. Before the reward shi, over the rst 11 days, pre-housing × post-housing × reward size aected the average run-time (P<0.05). All BB pigs ran slower than other pigs (BB: 59.3±2.8; BE: 35.9±1.7; EB: 39.6±2.2; EE: 40.9±2.2, P<0.05), without any other signicant pairwise dierences. Analysis per treatment revealed, however, that EB 6-reward pigs were faster than the 1-reward pigs. Overall latency was higher on the last days (P<0.001). Aer the reward size shied to 1 on day 12, pre-housing × post-housing aected the latency (P<0.001). Post hoc analysis showed that again, BB pigs were slower than other pigs (BB: 88.2±2.7; BE 62.3±2.3; EB: 57.3±2.3; EE: 70.4±2.6, P<0.001), and EB pigs were faster than EE pigs (P<0.05). Pigs ran slower aer than before the reward shi (P<0.001). Nevertheless, pigs in the 6-1 group ran slower than pigs in the 1-1 group aer the reward shi (6-1: 73.9±2.0; 1-1: 66.4±1.8, P<0.05), suggesting that pigs are sensitive to a loss in reward size. is was, however, irrespective of housing given the lack of interactions with reward size. We conclude that housing aected the latency to run down a runway for a reward in pigs, which can indicate a lower motivation in the BB pigs, an eect that was absent in the B pigs that switched to enriched housing (BE pigs). We found, however, no evidence that housing or a switch in housing conditions aected the sensitivity to reward loss.
Detection of candidate regions affecting bovine IgM natural antibodies in milk
Cordero Solorzano, Juan ; Arts, J.A.J. ; Parmentier, H.K. ; Bovenhuis, H. - \ 2018
In: Proceedings of the World Congress on Genetics Applied to Livestock Production. - WCGALP - 8 p.
Two genomic regions were found to be associated with IgM antibody titers in milk binding lipoteichoic acid (LTA); one on chromosome 17 and the other on chromosome 21. Phenotypes were measured by ELISA and genotypes consisted of imputed 777k SNP. Single SNP analyses were run using an animal model to retrieve significant SNP. In BTA21, a gene for the heavy chains of immunoglobulins, IGHV is proposed as a candidate gene as was true for VPREB3 in BTA17 related to B-cell maturation. These findings provide a further step in better understanding the genetic background of natural antibodies (NAbs), that may be relevant for relevant for the estimation of dairy cattle health. Keywords: dairy cattle, natural antibodies, genome-wide association
Candidate gene for natural immune competence in chickens revealed through genome wide association study
Visker, M.H.P.W. ; Berghof, T.V.L. ; Arts, J.A.J. ; Parmentier, H.K. ; Poel, J.J. van der; Vereijken, Addie ; Bovenhuis, H. - \ 2018
In: Proceedings of the World Congress on Genetics Applied to Livestock Production. - WCGALP - 4 p.
Natural antibodies (NAb) are antibodies that are present in individuals without prior exposure to the antigen that they bind. In chickens, NAb have been associated with survival. Furthermore, genetic variation between chickens in NAb levels has been demonstrated. To improve our understanding of the genetic variation for NAb levels in chickens we performed a genome wide association study. A population of 1,628 White Leghorn chickens was investigated. Animals were phenotyped for NAb levels using the antigen keyhole limpet hemocyanin (KLH) as model. Animals were genotyped for 15,579 single nucleotide polymorphisms (SNP). Highly significant association between SNP and NAb levels was observed on chicken chromosome 4. A single SNP was identified as the most likely candidate for this association. Identification of this candidate SNP was based on further analysis with full genome sequence genotypes and on predicted consequences of associated SNP. This candidate SNP causes a phenylalanine to leucine amino acid change in one of the leucine rich repeats in toll-like receptor 1 family member A (TLR1A). Our results suggest an important role for TLR1A in relation to NAb levels in chickens. Further study should confirm the effect of the SNP in TLR1A on NAb levels and on the (natural) immune competence of chickens. Keywords: natural antibodies, toll-like receptor, TLR1A
Early and later life environmental enrichment affect leukocyte subpopulations in pigs
Luo, Lu ; Hoek, A. ; Bolhuis, J.E. ; Parmentier, H.K. ; Kemp, B. ; Jansen, C.A. - \ 2018
Some thoughts on breeding for enhanced immunity in chicken. Consequences for vaccine responses?
Parmentier, Henk - \ 2018
Constraints of chicken immunity and vaccine responses?
Parmentier, Henk - \ 2018
Antigen-dependent effects of divergent selective breeding based on natural antibodies on specific humoral immune responses in chickens
Berghof, T.V.L. ; Arts, J.A.J. ; Bovenhuis, H. ; Lammers, A. ; Poel, J.J. van der; Parmentier, H.K. - \ 2018
Vaccine 36 (2018)11. - ISSN 0264-410X - p. 1444 - 1452.
Breeding - Chicken - General disease resistance - Keyhole limpet hemocyanin (KLH) - Natural antibody - Specific antibody
NAb are defined as antigen binding antibodies present without a known previous exposure to this antigen. NAb are suggested to enhance specific antibody (SpAb) responses, but consequences of different NAb levels on immunization are largely unknown. Layer chickens were divergently selected and bred for keyhole limpet hemocyanin (KLH)-binding NAb titers, resulting in a High line and a Low line. In this study, we investigated: (1) the relation of NAb levels with SpAb titers; and (2) the effect of immunization on NAb titers. The 50 highest females of the High line and the 50 lowest females of the Low line of generation 2 were intramuscularly immunized at 33 weeks of age with 1 mL phosphate buffered saline (PBS) containing one of four treatments: (1) negative control (no antigen), (2) 500 μg KLH, (3) 100 μg avian tuberculin purified protein derivative of Mycobacterium avium (PPD), or (4) 250 μg human serum albumin (HuSA). IgM and IgG titers of NAb and SpAb in plasma were determined prior to immunization and weekly for 5 weeks post immunization by indirect ELISA. In addition, antibody affinity was investigated. No differences in SpAb and NAb response against KLH and PPD were observed as a consequence of different NAb titers, but increased and prolonged SpAb and NAb titer responses against HuSA were observed for the High line compared to the Low line. Different natural antibody titers did not impair SpAb dynamics and SpAb affinity. NAb titers were not, or for only short-term, affected by immunization. We show here that NAb may enhance SpAb responses, but that this effect is antigen-dependent. We hypothesize that NAb play a role in general disease resistance through enhancement of the humoral adaptive immune response.
Genomic Region Containing Toll-Like Receptor Genes Has a Major Impact on Total IgM Antibodies Including KLH-Binding IgM Natural Antibodies in Chickens
Berghof, T.V.L. ; Visker, M.H.P.W. ; Arts, J.A.J. ; Parmentier, H.K. ; Poel, J.J. van der; Vereijken, A.L.J. ; Bovenhuis, H. - \ 2018
Frontiers in Immunology 8 (2018). - ISSN 1664-3224 - 14 p.
Natural antibodies (NAb) are antigen binding antibodies present in individuals without a previous exposure to this antigen. Keyhole limpet hemocyanin (KLH)-binding NAb levels were previously associated with survival in chickens. This suggests that selective breeding for KLH-binding NAb may increase survival by means of improved general disease resistance. Genome-wide association studies (GWAS) were performed to identify genes underlying genetic variation in NAb levels. The studied population consisted of 1,628 adolescent layer chickens with observations for titers of KLH-binding NAb of the isotypes IgM, IgA, IgG, the total KLH-binding (IgT) NAb titers, total antibody concentrations of the isotypes IgM, IgA, IgG, and the total antibodies concentration in plasma. GWAS were performed using 57,636 single-nucleotide polymorphisms (SNP). One chromosomal region on chromosome 4 was associated with KLH-binding IgT NAb, and total IgM concentration, and especially with KLH-binding IgM NAb. The region of interest was fine mapped by imputing the region of the study population to whole genome sequence, and subsequently performing an association study using the imputed sequence variants. 16 candidate genes were identified, of which FAM114A1, Toll-like receptor 1 family member B (TLR1B), TLR1A, Krüppel-like factor 3 (KLF3) showed the strongest associations. SNP located in coding regions of the candidate genes were checked for predicted changes in protein functioning. One SNP (at 69,965,939 base pairs) received the maximum impact score from two independent prediction tools, which makes this SNP the most likely causal variant. This SNP is located in TLR1A, which suggests a fundamental role of TLR1A on regulation of IgM levels (i.e., KLH-binding IgM NAb, and total IgM concentration), or B cells biology, or both. This study contributes to increased understanding of (genetic) regulation of KLH-binding NAb levels, and total antibody concentrations.
Reduced mortality after avian pathogenic Escherichia coli inoculation in chickens bred for high natural antibodies
Berghof, T.V.L. ; Matthijs, M.G.R. ; Arts, J.A.J. ; Bovenhuis, H. ; Dwars, R.M. ; Poel, J.J. van der; Parmentier, H.K. - \ 2017
Effect of enriched housing on levels of natural (auto-)antibodies in pigs co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and Actinobacillus pleuropneumoniae
Luo, Lu ; Dixhoorn, I.D.E. van; Reimert, I. ; Kemp, B. ; Bolhuis, J.E. ; Parmentier, H.K. - \ 2017
Veterinary Research 48 (2017). - ISSN 0928-4249 - 10 p.
Housing of pigs in barren, stimulus-poor housing conditions may influence their immune status, including antibody responses to (auto-)antigens, and thus affect immune protection, which will influence the onset and outcome of infection. In the present study, we investigated the effects of environmental enrichment versus barren housing on the level of natural (auto-)antibodies (NA(A)b) and their isotypes (IgM and IgG) binding keyhole limpet hemocyanin (KLH), myelin basic protein (MBP), and phosphorycholine conjugated to bovine serum albumin (PC-BSA) in pigs co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and Actinobacillus pleuropneumoniae (A. pleuropneumoniae). Pigs (n = 56) were housed in either barren or enriched pens from birth to 54 days of age. They were infected with PRRSV on 44 days of age, and with A. pleuropneumoniae 8 days later. Blood samples were taken on 7 different sampling days. Housing significantly affected the overall serum levels of NA(A)b binding KLH, MBP and PC-BSA, and before infection barren housed pigs had significantly higher levels of NA(A)b than enriched housed pigs, except for KLH-IgM and PC-BSA-IgG. Infection only affected the IgM, but not the IgG isotype. Moreover, changes in MBP-IgM and PC-BSA-IgM following infection were different for enriched and barren housed pigs. These results suggest that the effect of infection on NA(A)b is influenced by housing conditions and that NA(A)b, especially IgM may be affected by infection.
Selectie van hennen voor ziekteresistentie op basis van natuurlijke antilichamen
Parmentier, Henk - \ 2017
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