Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Systemic colonization of potato plants resulting from potato haulm inoculation with Dickeya solani or Pectobacterium parmentieri
    Kastelein, P. ; Forch, M.G. ; Krijger, M.C. ; Zouwen, P.S. van der; Berg, W. van den; Wolf, J.M. van der - \ 2020
    Canadian Journal of Plant Pathology = Revue Canadienne de Phytopathologie (2020). - ISSN 0706-0661 - p. 1 - 15.
    blackleg - Confocal laser scanning microscopy - fluorescence microscopy - GFP-tagged strains - haulm destruction - leaf wounding - slow wilt
    In two glasshouse experiments, colonization of potato (Solanum tuberosum L.) plants by the bacterial pathogens Dickeya solani and Pectobacterium parmentieri was studied after leaf infection. Leaves, whether or not artificially wounded, were spray-inoculated with various densities of green-fluorescent protein tagged strains of the pathogens, avoiding contamination of soil during inoculation. Microscopy analysis indicated that both pathogens were able to penetrate and colonize hydathodes, stomata and wounds of inoculated leaves. Dickeya solani was detected at 42 days after inoculation in leaves, stems, stolons and occasionally in tubers, whereas P. parmentieri was restricted to leaves, stems and stolons, and could not be detected in tubers. The infection percentage was higher for plants with wounded leaves than for plants with untouched leaves, and higher at higher inoculum densities. Nevertheless, infection of leaves could also occur at low densities of D. solani (102 cfu mL−1). We further investigated the risks for translocation of the pathogens from infected haulms through soil into progeny tubers after haulm destruction. In a glasshouse experiment, populations of the pathogens increased in haulms in the first week after chemical or mechanical destruction, but decreased in the second week. For P. parmentieri, transmission occurred from destroyed haulms via soil into progeny tubers in soil, but not for D. solani.
    Development and validation of IPM strategies for the cultivation of cisgenically modified late blight resistant potato
    Kessel, Geert J.T. ; Mullins, Ewen ; Evenhuis, Albartus ; Stellingwerf, Jeroen ; Cortes, Vilma Ortiz ; Phelan, Sinead ; Bosch, Trudy van den; Förch, Marieke G. ; Goedhart, Paul ; Voet, Hilko van der; Lotz, Lambertus A.P. - \ 2018
    European Journal of Agronomy 96 (2018). - ISSN 1161-0301 - p. 146 - 155.
    Cisgenic modification - GM potato - Host resistance - Integrated pest management - Phytophthora infestans
    Potato late blight disease remains the primary stressor of commercial potato production across the EU, typically requiring >10 fungicide applications per growing season to offset crop losses. In response, the goal of this study was to test and validate a novel, more durable, control strategy for potato late blight. This IPM2.0 strategy is based on the principles of Integrated Pest Management (IPM) which sees the deployment of a late blight resistant potato genotype, a cisgenically modified, Desiree based resistant potato line here, in conjunction with pathogen population monitoring for virulence to the resistance genes (R genes) deployed and a “do not spray unless”, low input fungicide spray strategy. Field evaluations were completed in the Netherlands and in Ireland in 2013, 2014 and in Ireland in 2015. Comparators used in this study included the original but susceptible potato variety Desiree and the conventional but highly resistant variety Sarpo Mira. The novel IPM2.0 strategy was compared to local common practice (fungicide applications on a near weekly basis) and an untreated control. Overall, the IPM2.0 control strategy validated here reduced the average fungicide input by 80–90% without compromising control efficacy. Corresponding environmental side-effects were reduced proportionally. The results underline the pragmatic role host resistance can provide to commercial potato production systems and to society at large if employed as part of an integrated late blight control system.
    Fifteenth EuroBlight Workshop
    Forch, Marieke - \ 2015
    phytophthora - EU-monitoring - euroblight
    The eu-monitoring project, a view behind the scenes
    Enhancing disease suppression in substrates for strawberry cultivation
    Postma, J. ; Forch, M.G. - \ 2015
    REFERTIL
    Compost
    The use of mycorrhiza for increasing compost and biochar quality : REFERTIL Newsletter, Issue March 2015, Page 4
    Postma, J. ; Nijhuis, E.H. ; Forch, M.G. - \ 2015
    REFERTIL
    Long term storage of Alternaria
    Bosch, G.B.M. van den; Forch, M.G. ; Evenhuis, A. - \ 2014
    - 1 p.
    First results of an Eu-wide genotype monitoring of Phytophthora infestans using FTA-cards
    Meier-Runge, F. ; Bosch, G.B.M. van den; Forch, M.G. ; Evenhuis, A. ; Kessel, G.J.T. - \ 2014
    In: Proceedings of the fourteenth Euroblight Workshop. - PPO (PPO-Special Report 16)
    Screening for Alternaria (solani or alternata)
    Forch, M.G. ; Bosch, G.B.M. van den - \ 2014
    Mycorrhiza for fighting Phytophthora in strawberries
    Postma, J. ; Förch, M.G. - \ 2014
    Wageningen : Wageningen UR
    Mycorrhiza inzetten tegen Phytophthora in aardbei
    Postma, J. ; Förch, M.G. - \ 2014
    Wageningen : Wageningen UR
    The risks of air-borne inoculum on infections of tubers with Dickeya solani and Pectobacterium wasabiae
    Wolf, J.M. van der; Kastelein, P. ; Förch, M.G. ; Krijger, M.C. - \ 2014
    Deltaplan Erwinia. Deel C - Pootaardappelen. Eindrapport van het onderzoek 2009 - 2012
    Boomsma, D. ; Velvis, H. ; Kristelijn, K. ; Tent Becking, T. van; Kastelein, P. ; Zouwen, P.S. van der; Krijger, M.C. ; Förch, M.G. ; Wolf, J.M. van der; Czajkowski, R.L. ; Jafra, S. ; Bovenkamp, G. van den; Haan, E. de; Nunes Leite, L. - \ 2013
    Den Haag, Zoetermeer : Nederlands Aardappel Organisatie (NAO), Productschap Akkerbouw (PA) - 104
    erwinia - pectobacterium - dickeya - plantenziekteverwekkende bacteriën - plantenziekten - plantenziektebestrijding - aardappelen - akkerbouw - landbouwkundig onderzoek - besmetting - ziektepreventie - gewasbescherming - erwinia - pectobacterium - dickeya - plant pathogenic bacteria - plant diseases - plant disease control - potatoes - arable farming - agricultural research - contamination - disease prevention - plant protection
    Het Deltaplan Erwinia (Deel C – Pootaardappelen) is een vervolg op het project 'Bacterievrije pootgoedteelt - een uitdaging!'. Het doel was om meer inzicht te krijgen in de Erwinia-problemen in de pootgoedteelt en uiteindelijk te komen tot een reductie van de economische schade met 50%. Uit het onderzoek (2009-2012) blijkt dat er bij vrijwel elk onderdeel van de teeltcyclus van pootaardappelen risicovolle handelingen vallen aan te wijzen die tot verergering van het probleem leiden. Na vier jaar onderzoek is de conclusie dat er, om de ten doel gestelde reductie van 50% reductie te realiseren, een samenhangend pakket aan maatregelen nodig is. Daarvoor wordt een aantal aanbevelingen gedaan
    Population dynamics of Phytophthora infestans in the Netherlands reveals expansion and spread of dominant clonal lineages and virulence in sexual offspring
    Li, Y. ; Lee, T.A.J. van der; Evenhuis, A. ; Bosch, G.B.M. van den; Bekkum, P.J. van; Förch, M.G. ; Gent-Pelzer, M.P.E. van; Raaij, H.M.G. van; Jacobsen, E. ; Huang, S.W. ; Govers, F. ; Vleeshouwers, V.G.A.A. ; Kessel, G.J.T. - \ 2012
    G3 : Genes Genomes Genetics 2 (2012). - ISSN 2160-1836 - p. 1529 - 1540.
    broad-spectrum resistance - genetics data-analysis - microsatellite markers - genotypic diversity - mating-type - late blight - potato - reproduction - migration - progeny
    For a comprehensive survey of the structure and dynamics of the Dutch Phytophthora infestans population, 652 P. infestans isolates were collected from commercial potato fields in the Netherlands during the 10-year period 2000–2009. Genotyping was performed using 12 highly informative microsatellite markers and mitochondrial haplotypes. In addition, for each isolate, the mating type was determined. STRUCTURE analysis grouped the 322 identified genotypes in three clusters. Cluster 1 consists of a single clonal lineage NL-001, known as “Blue_13”; all isolates in this cluster have the A2 mating type and the Ia mitochondrial haplotype. Clusters 2 and 3 display a more elaborate substructure containing many unique genotypes. In Cluster 3, several distinct clonal lineages were also identified. This survey witnesses that the Dutch population underwent dramatic changes in the 10 years under study. The most notable change was the emergence and spread of A2 mating type strain NL-001 (or “Blue_13”). The results emphasize the importance of the sexual cycle in generating genetic diversity and the importance of the asexual cycle as the propagation and dispersal mechanism for successful genotypes. Isolates were also screened for absence of the Avrblb1/ipiO class I gene, which is indicative for virulence on Rpi-blb1. This is also the first report of Rpi-blb1 breakers in the Netherlands. Superimposing the virulence screening on the SSR genetic backbone indicates that lack the Avrblb1/ipiO class I gene only occurred in sexual progeny. So far, the asexual spread of the virulent isolates identified has been limited.
    Gevoeligheid van twee cohorten P. infestans-isolaten voor Shirlan, Thema: Phytophthora infestans cohorten P. infestans-isolaten zijn, als vertegenwoordigers
    Kessel, G.J.T. ; Forch, M.G. ; Bosch, T. van den; Bekkum, P.J. van; Zande, J.C. van de; Evenhuis, A. - \ 2011
    S.n.
    phytophthora infestans - gewasbescherming - aardappelen - controle - teeltsystemen - phytophthora infestans - plant protection - potatoes - control - cropping systems
    Informatieposter getiteld "Gevoeligheid van twee cohorten P. infestians-isolaten voor Shirlan", thema Phytophtera infestans. Naar aanleiding van problemen met de beheersing van Phytophthora tijdens de zeer natte maanden augustus en september 2010 werd door de sector een vermoeden geuit dat de huidige P. infestans-populatie, na bijna 2 decennia blootstelling, mogelijk minder gevoelig geworden was voor het fungicide Shirlan (a.i. fluazinam)
    Monitoring the Dutch Phytophthora infestans population for virulence against new R-genes
    Förch, M.G. ; Bosch, G.B.M. van den; Bekkum, P.J. van; Evenhuis, B. ; Vossen, J. ; Kessel, G.J.T. - \ 2010
    In: Proceedings of the Twelfth EuroBlight (A potato late blight network for Europe) workshop, Arras, France, 3-6 May 2010. - Lelystad : Applied Plant Research - p. 45 - 50.
    New possibilities offered by marker assisted breeding and GMO breeding have sparked renewed international efforts to breed for durable potato late blight resistance. Phytophthora infestans is however known for its adaptability, a trait confirmed by recent discoveries on the structure of the P. infestans genome. One of the possibilities to enhance the durability of newly introduced host resistance is to monitor the pathogen population for virulence to new R genes, prior to - and after their introduction. The late blight control strategy should be adapted accordingly. The Dutch P. infestans population was monitored during the growing seasons 2006 – 2008. P. infestans isolates were collected from blighted production fields and from bait fields in which R gene containing potato clones were grown without fungicide protection. A selection of the P. infestans isolates collected were characterized for virulence to a range of new R genes using a detached leaf bio-assay. Virulence for all single R genes tested was found. When we focus on R genes Rpi-blb1 and Rpi-blb2, no virulence was found in 2006. One Rpi-blb1 virulent isolate was found in 2007. Another 2007 isolate was found to be virulent to Rpi-blb2. Depending on the genetic background in which Rpi-blb1 was placed 13 or 21 isolates were virulent in 2008. Depending on the genetic background in which Rpi-blb2 was placed 4 or 11 isolates were virulent in 2008. One isolate was found to infect the stacked Rpi-blb1 and Rpi-blb2 resistance genes in a detached leaf assay. From these findings it is recommended that monitoring systems should be part of future potato late blight control strategies. The resulting information on the dynamics of virulence within the local P. infestans population can then be used to enhance the durability of newly introduced host resistance
    Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes
    Förch, M.G. ; Bosch, G.B.M. van den; Bekkum, P.J. van; Evenhuis, A. ; Vossen, J.H. - \ 2010
    In: Proceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France. - Lelystad : Applied Plant Research - p. 45 - 49.
    New possibilities offered by marker assisted breeding and GMO breeding have sparked renewed international efforts to breed for durable potato late blight resistance. Phytophthora infestans is however known for its adaptability, a trait confirmed by recent discoveries on the structure of the P. infestans genome. One of the possibilities to enhance the durability of newly introduced host resistance is to monitor the pathogen population for virulence to new R genes, prior to - and after their introduction. The late blight control strategy should be adapted accordingly. The Dutch P. infestans population was monitored during the growing seasons 2006 – 2008. P. infestans isolates were collected from blighted production fields and from bait fields in which R gene containing potato clones were grown without fungicide protection. A selection of the P. infestans isolates collected were characterized for virulence to a range of new R genes using a detached leaf bio-assay. Virulence for all single R genes tested was found. When we focus on R genes Rpi-blb1 and Rpi-blb2, no virulence was found in 2006. One Rpi-blb1 virulent isolate was found in 2007. Another 2007 isolate was found to be virulent to Rpi-blb2. Depending on the genetic background in which Rpi-blb1 was placed 13 or 21 isolates were virulent in 2008. Depending on the genetic background in which Rpi-blb2 was placed 4 or 11 isolates were virulent in 2008. One isolate was found to infect the stacked Rpi-blb1 and Rpi-blb2 resistance genes in a detached leaf assay. From these findings it is recommended that monitoring systems should be part of future potato late blight control strategies. The resulting information on the dynamics of virulence within the local P. infestans population can then be used to enhance the durability of newly introduced host resistance
    Loofresistentie tegen Phytophthora infestans in aardappel : effecten van isolaatkeuze en proefontwerp op resistentiecijfers
    Kessel, G.J.T. ; Bosch, G.B.M. van den; Forch, M.G. ; Evenhuis, B. ; Flier, W.G. - \ 2010
    Wageningen : Plant Research International, Business Unit Biointeracties en Plantgezondheid (Rapport / Plant Research International 358) - 34
    solanum tuberosum - aardappelen - phytophthora infestans - ziekteresistentie - rassen (planten) - rassenproeven - entstof - solanum tuberosum - potatoes - phytophthora infestans - disease resistance - varieties - variety trials - inoculum
    Gedurende de periode 2002 tot en met 2004 werden veld- en laboratoriumproven uitgevoerd naar de effecten van de toetsmethode en isolaatkeuze op het resulterende Phytophthoraloofresisentiecijfer voor 30 economisch belangrijke rassen in nederland. Twee methodieken, de CGO methode en een alternatieve methode, werden vergeleken. Daarnaast werden de effecten van drie verschllende inocula die in meer of mindere mate representatief waren voor de Nederlandse P. infestans populatie vergeleken.
    New Phytophthora populations: A shift from indirect to direct sporangial germination?
    Kessel, G.J.T. ; Veloso, S. ; Forch, M.G. ; Latorse, M.P. - \ 2009
    In: Proceedings of the Eleventh EuroBlight Workshop, Hamar, Norway, 28-31 October 2008. - Lelystad, The Netherlands : Applied Plant Research, AGV Research Unit - p. 171 - 176.
    Phytophthora infestans, the causal agent of potato- and tomato late blight, remains a serious threat for (commercial) potato and tomato production. In North Western Europe, frequent fungicide applications, mostly aimed to prevent infection, form the back bone of potato late blight control. Modern protectants such as Shirlan (a.i. fluazinam) are highly effective against (germinating) P. infestans sporangia and zoospores. Zoospores in particular are so sensitive to low concentrations that the many applications over the past two decades may well have exerted sufficient selection to pressure against the formation of zoospores. Thus, over the years the balance between direct and indirect germination may have shifted towards direct germination. This hypothesis was investigated at Bayer Crop Science and Plant Research International
    Phytophthora: kampioen aanpassing
    Kessel, G.J.T. ; Evenhuis, A. ; Bosch, G.B.M. van den; Förch, M.G. ; Topper, C.G. - \ 2009
    phytophthora infestans - genotypen - fenotypen - virulentie - fungiciden - aardappelen - ziekteresistentie - phytophthora infestans - genotypes - phenotypes - virulence - fungicides - potatoes - disease resistance
    Poster met onderzoeksinformatie. Uit onbeschermd opgekweekte proefveldjes zijn Phytophthora infestans isolaten verzameld en gekarakteriseerd.
    Monitoring van Phytophthora infestans als 'early warning'
    Kessel, G.J.T. ; Evenhuis, A. ; Bosch, G.B.M. van den; Förch, M.G. ; Spits, H.G. ; Topper, C.G. - \ 2009
    phytophthora infestans - aardappelen - gewasbescherming - phytophthora infestans - potatoes - plant protection
    Poster met informatie uit het onderzoek naar het vroegtijdig opsporen van aanpassingen binnen de Nederlandse P. infestans-populatie m.b.t. waardplantresistentie, fungicidenresistentie en andere fenotypische en genotypische karakteristieken
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