Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

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Occurence of extended-spectrum Beta-lactamase producing E. coli in wild birds from the Netherlands
Veldman, K.T. ; Tulden, P. ; Kant, A. ; Testerink, J.J. ; Mevius, D.J. - \ 2013
Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands
Veldman, K.T. ; Tulden, P. ; Kant, A. ; Testerink, J.J. ; Mevius, D.J. - \ 2013
Applied and Environmental Microbiology 79 (2013)24. - ISSN 0099-2240 - p. 7556 - 7561.
spectrum-beta-lactamase - extended-spectrum - antibiotic-resistance - natural reserve - ctx-m - salmonella - plasmids - gulls - enterobacteriaceae - environments
Cloacal swabs from carcasses of Dutch wild birds obtained in 2010 and 2011 were selectively cultured on media with cefotaxime to screen for the presence of extended-spectrum beta-lactamase (ESBL)/AmpC-producing Escherichia coli. Subsequently, all cefotaxime-resistant E. coli isolates were tested by broth microdilution and microarray. The presence of ESBL/AmpC and coexisting plasmid-mediated quinolone resistance (PMQR) genes was confirmed by PCR and sequencing. To determine the size of plasmids and the location of ESBL and PMQR genes, S1 pulsed-field gel electrophoresis (PFGE) was performed on transformants, followed by Southern blot hybridization. The study included 414 cloacal swabs originating from 55 different bird species. Cefotaxime-resistant E. coli isolates were identified in 65 birds (15.7%) from 21 different species. In all, 65 cefotaxime-resistant E. coli ESBL/AmpC genes were detected, mainly comprising variants of bla(CTX-M) and bla(CMY-2). Furthermore, PMQR genes [aac(6')-lb-cr, qnrB1, and qnrS1] coincided in seven cefotaxime-resistant E. coli isolates. Overall, replicon typing of the ESBL/AmpC-carrying plasmids demonstrated the predominant presence of IncI1 (n = 31) and variants of IncF (n = 18). Our results indicate a wide dissemination of ESBL and AmpC genes in wild birds from The Netherlands, especially among aquatic-associated species (waterfowl, gulls, and waders). The identified genes and plasmids reflect the genes found predominantly in livestock animals as well as in humans.
Antibiotic resistance in E. coli obtained from wild birds in the Netherlands
Veldman, K.T. ; Kant, A. ; Testerink, J.J. ; Tulden, P.W. van; Mevius, D.J. - \ 2012
Onderzoek naar de microbiologische kwaliteit van drinkwater bij landbouwhuisdieren
Veldman, K.T. ; Wielen, van der, E. ; Putirulan, F.F. ; Testerink, J.J. ; Mevius, D.J. - \ 2012
Lelystad : CVI
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