Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Research news : new enzyme technologies for fibre modification
Valk, H.C.P.M. van der - \ 2008
enzymen - natuurlijke vezels - verwerking - wetenschappelijk onderzoek - biobased economy - enzymes - natural fibres - processing - scientific research
Poster met informatie over het onderzoek dat de Agrotechnology & Food Sciences Group (AFSG) van Wageningen UR uitvoert naar de modificatie van plantenvezels. AFSG richt zich op de ontwikkeling van nieuwe markten en nieuwe toepassingen van (hernieuwbare) biologische grondstoffen, gecombineerd met fundamenteel en en toegepast onderzoek.
Isolation and characterization of a tomato non-specific lipid transfer protein involved in polygalacturonase-mediated pectin degradation
Tomassen, M.M.M. ; Barrett, D.M. ; Valk, H.C.P.M. van der; Woltering, E.J. - \ 2007
Journal of Experimental Botany 58 (2007)5. - ISSN 0022-0957 - p. 1151 - 1160.
beta-subunit - gene-expression - transgenic tomatoes - fruit - isoenzymes - plant - converter - ultrastructure - manipulation - conversion
An important aspect of the ripening process of tomato fruit is softening. Softening is accompanied by hydrolysis of the pectin in the cell wall by pectinases, causing loss of cell adhesion in the middle lamella. One of the most significant pectin-degrading enzymes is polygalacturonase (PG). Previous reports have shown that PG in tomato may exist in different forms (PG1, PG2a, PG2b, and PGx) commonly referred to as PG isoenzymes. The gene product PG2 is differentially glycosylated and is thought to associate with other proteins to form PG1 and PGx. This association is thought to modulate its pectin-degrading activity in planta. An 8 kDa protein that is part of the tomato PG1 multiprotein complex has been isolated, purified, and functionally characterized. This protein, designated `activator¿ (ACT), belongs to the class of non-specific lipid transfer proteins (nsLTPs). ACT is capable of `converting¿ the gene product PG2 into a more active and heat-stable form, which increases PG-mediated pectin degradation in vitro and stimulates PG-mediated tissue breakdown in planta. This finding suggests a new, not previously identified, function for nsLTPs in the modification of hydrolytic enzyme activity. It is proposed that ACT plays a role in the modulation of PG activity during tomato fruit softening.
Antisense down-regulation of strawberry endo-beta (1,4)-glucanase genes does not prevent fruit softening during ripening
Palomer, X. ; Llop-Tous, I. ; Vendrell, M. ; Krens, F.A. ; Schaart, J.G. ; Boone, M.J. ; Valk, H.C.P.M. van der; Salentijn, E.M.J. - \ 2006
Plant Science 171 (2006)5. - ISSN 0168-9452 - p. 640 - 646.
cell-wall polysaccharides - transgenic tomato fruit - fragaria x ananassa - molecular characterization - pectate lyase - higher-plants - expression - endo-1,4-beta-glucanase - endo-beta-1,4-glucanase - suppression
Strawberry (Fragaria × ananassa Duch.) fruit softening during ripening is associated with the overlapping presence of two divergent endo-ß-(1,4)-glucanases (EC 3.2.1.4; EGases), Cel1 and Cel2. Antisense down-regulation of both genes was performed to assess the precise role of these endo-ß-(1,4)-glucanases on strawberry fruit softening. Constant down-regulation of cel1 expression throughout ripening was obtained, which was accompanied by reduced Cel1 protein accumulation. However, diminution of the Cel1 protein level together with a reduction of the total EGase activity to 40% of the control level did not affect fruit firmness, thus suggesting that Cel1 protein is not the major determinant of fruit softening during ripening. On the other hand, no significant reduction of Cel2 protein accumulation was found in any of the Cel2 transgenic or Cel1/Cel2 double-transgenic lines obtained. The difficulties encountered to yield a strawberry line with significant reduction of cel2 expression suggest that this gene might be playing a pivotal role on fruit development prior to ripening, thus accounting for the lack of Cel2 protein down-regulation observed
Biotechnological application of enzymes for making paper pulp from green jute/kenaf
Snijder, M.H.B. ; Lips, S.J.J. ; Maas, R.H.W. ; Kamp, R.G.M. op den; Valk, H.C.P.M. van der; Jong, E. de - \ 2004
Wageningen : Agrotechnology & Food Innovations (Rapport 93)
papier - pulpbereiding - jute - kenaf - productieprocessen - biotechnologie - voorbehandeling - enzymen - biobased economy - paper - pulping - production processes - biotechnology - pretreatment - enzymes
The objectives of the tasks of Agrotechnology & Food Innovation (formerly ATO) in the project are: to determine at laboratory level the best conditions for producing high-yield pulps from whole jute for utilisation in wood containing papers; to test the potential of using recommended enzyme recipes in both extruder and refiner processes in terms of pulp quality, chemicals and energy consumption, brightness and production cost; to evaluate the potential of a micro-biological pre-treatment with selected strains of fungi; to establish process conditions for pilot confirmatory trials.
High-yield pulps from whole jute for making paper
Snijder, M.H.B. ; Lips, S.J.J. ; Maas, R.H.W. ; Kamp, R.G.M. op den; Valk, H.C.P.M. van der; Jong, E. de - \ 2004
In: 2nd Dissemination Workshop on Biotechnological application of enzymes for making paper pulp from green jute / Kenaf, Hyderabad, A.P., India, 18 - 19 November, 2004. - Saharanpur : Central Pulp and Paper Research Institute - p. 16 - 18.
Modulation of cell wall structure and composition using a fungal cellulose-binding domain
Quentin, M.G.E. ; Indemans, M.H.G. ; Derksen, J. ; Jong, E. de; Valk, H.C.P.M. van der - \ 2004
In: Wood fibre cell walls : methods to study their formation, structure and properties / Schmitt, U., Ander, P., Barnett, J.R., Emons, A.M.C., Jeronimidis, G., Saranpää, P., Tschegg, S., Uppsala : Swedish University of Agricultural Sciences - ISBN 9789157668035 - p. 45 - 56.
Cellulose-binding domains: tools for innovation in cellulosic fibre production and modification
Quentin, M.G.E. ; Valk, H.C.P.M. van der; Dam, J.E.G. van; Jong, E. de - \ 2003
ACS symposium series 855 (2003). - ISSN 0097-6156 - p. 132 - 155.
cellulose - cellulosevezels - hemicellulosen - enzymen - biobased economy - cellulosic fibres - hemicelluloses - enzymes - reesei cellobiohydrolase-i - plant-cell walls - nuclear-magnetic-resonance - non-hydrolytic disruption - bleached kraft fibers - trichoderma-reesei - crystalline cellulose - cotton fibers - endo-1,4-beta-glucanase cel1 - arabidopsis-thaliana
Plant cell walls are composed of cellulose, nature's most abundant macromolecule, and therefore represent a renewable resource of special technical importance. Cellulose degrading enzymes involved in plant cell wall loosening (expansins), or produced by plant pathogenic microorganisms (cellulases), share similarities favouring the degradation of this highly crystalline substrate. Most of the cellulases and cell wall loosening expansins share a multi-domain structure, which includes a cellulose-binding domain (CBD). CBDs possess the intrinsic ability to strongly and specifically bind to cellulose. CBDs may be applied to engineer hybrid enzymes able to bind to cellulose on one end, and to display enzymatic or chemical reactivity on the other, providing innovative solutions to modify cellulosic surfaces or to immobilise biocatalysts on it. In transgenic plants, CBDs influence polysaccharide synthesis and their assembly in the cell wall. Therefore, CBDs represent biotechnological tools to modify cellulosic fibres either during their growth or during post harvest processing.
Plant cell walls are composed of cellulose, nature's most abundant macromolecule, and therefore represent a renewable resource of special technical importance. Cellulose degrading enzymes involved in plant cell wall loosening (expansins), or produced by plant pathogenic microorganisms (cellulases), share similarities favouring the degradation of this highly crystalline substrate. Most of the cellulases and cell wall loosening expansins share a multi-domain structure, which includes a cellulose-binding domain (CBD). CBDs possess the intrinsic ability to strongly and specifically bind to cellulose. CBDs may be applied to engineer hybrid enzymes able to bind to cellulose on one end, and to display enzymatic or chemical reactivity on the other, providing innovative solutions to modify cellulosic surfaces or to immobilise biocatalysts on it. In transgenic plants, CBDs influence polysaccharide synthesis and their assembly in the cell wall. Therefore, CBDs represent biotechnological tools to modify cellulosic fibres either during their growth or during post harvest processing.
Smaak en verbetering van afzetmogelijkheden van Nederlandse appelen : verbetering van de smaak van appel middels specifieke naoogstbehandeling ter verbetering van het imago en de afzet : verslag onderzoeksproject januari 1996 - januari 1998
Tomassen, M.M.M. ; Valk, H.C.P.M. ; Kooten, O. van; Leguijt, T. ; Oosterhaven, J. ; Sanders, M.G. ; Termeer, A.G.W. ; Verschoor, J.A. ; Vuurst de Vries, R.G. van de; Wild, J.P.J. de - \ 1998
Wageningen : Agrotechnologisch Onderzoek Instituut (ATO-DLO) - 79
Optimale afgiftetemperaturen van verse groenten en fruit
Boerrigter, Henry ; Oosterhaven, Koos ; Polderdijk, Anneke ; Schaik, Alex van; Schouten, Simon ; Valk, Henry van der; Wild, Hans de - \ 1998
Wageningen : Agrotechnologisch Onderzoek Instituut (ATO-DLO) (Rapport / Instituut voor Agrotechnologisch Onderzoek, Dienst Landbouwkundig Onderzoek (ATO-DLO) B297) - 26
Laser-investigation of ethylene emission by fruits
Vries, H.S.M. de; Harren, F.J.M. ; Woltering, E.J. ; Valk, H.C.P.M. van der; Reuss, J. - \ 1998
In: Proceedings of the sixth international symposium of the European Concerted Action Program (COST94), The post-harvest treatment of fruit and vegetables: Current status and future prospects - p. 259 - 267.
Physiological and biochemical changes in cell walls of apple and tomato during storage and ripening
Valk, H.C.P.M. van der; Tomassen, M.M.M. ; Donkers, J.W. - \ 1998
In: Proceedings of the sixth international symposium of the European Concerted Action Program (COST94), The post-harvest treatment of fruit and vegetables: Current status and future prospects - p. 363 - 368.
Optimale afgiftetemperaturen van verse groenten en fruit
Boerrigter, Henry ; Oosterhaven, Koos ; Polderdijk, Anneke ; Schaik, Alex van; Schouten, Simon ; Valk, Henry van der; Wild, Hans de - \ 1997
Wageningen : ATO-DLO (Rapport / ATO-DLO B297) - 24
Physiological and Biochemical Changes in Cell Walls of Apple and Tomato Fruit during Storage and Ripening
Valk, H.C.P.M. van der; Tomassen, M.M.M. ; Donkers, J.W. - \ 1997
In: The Postharvest Treatment of Fruit and Vegetables - Current Status and Future Prospects. Proceedings of the 6th International Symposium of the European Concerted Action Program COST 94, 19-22 october 1994, Oosterbeek, NL - p. 363 - 367.
Mushrooms: more than just taste (in Dutch)
Leguijt, C. ; Valk, H.C.P.M. van der; Sanders, M.G. ; Termeer, A.G.W. ; Vuurst de Vries, R.G. van der; Yüksel, D. ; Wichers, H.J. - \ 1997
De Champignoncultuur 41 (1997)8. - ISSN 0009-1316 - p. 277 - 285.
agaricus - chemische structuur - relaties - organoleptische kenmerken - markten - marketing - marktconcurrentie - aanbodsevenwicht - tuinbouwgewassen - biologische eigenschappen - agaricus - chemical structure - relationships - organoleptic traits - markets - marketing - market competition - supply balance - horticultural crops - biological properties
Het versterken van de positie van Nederlandse champignonbedrijven is nodig om de afzetmogelijkheden te verruimen. Drie deelprojecten zijn genoemd die in het kader van de afzet worden uitgevoerd. Het eerste dat gaat over de karakterisering van smaak, geur en textuur van verse champignons is in dit artikel uiteengezet
Ethylene and CO2 emission pathways in fruits investigated by infrared absorption techniques
Vries, H.S.M. de; Wasono, M.A.J. ; Harren, F.J.M. ; Woltering, E.J. ; Valk, H.C.P.M. van der; Reuss, J. - \ 1996
Postharvest Biology and Technology 8 (1996). - ISSN 0925-5214 - p. 1 - 10.
The tomatometer, an instrument of firmness of tomatoes
Polderdijk, J.J. ; Tijskens, L.M.M. ; Robbers, J.E. ; Valk, H.C.P.M. van der - \ 1995
In: COST94 proceedings, Systems and operations for post harvest quality - p. 49 - 51.
Investigation of local ethylene emission from intact cherry tomatoes by means of photothermal deflection and photoacoustic detection
Vries, H.S.M. de; Harren, F.J.M. ; Voesenek, L.A.C.J. ; Blom, C.W.P.M. ; Woltering, E.J. ; Valk, H.C.P.M. van der; Reuss, J. - \ 1995
Plant Physiology 107 (1995). - ISSN 0032-0889 - p. 1371 - 1377.
Haalbaarheidsstudie : ontwikkeling en toepassing van een praktijknaoogst-modified atmosphere systeem voor de Nederlandse tomaat voor verbetering van stevigheid, kwaliteit, smaak, transport- en bewaarmogelijkheden : rapportage 2e fase; maart-juni 1994
Valk, Henry van der; Boerrigter, Henry ; Robbers, Jan ; Boogaard, Gérard van den; Evelo, Rob - \ 1994
Wageningen : Agrotechnologisch Onderzoek Instituut (ATO-DLO) (Rapport / Instituut voor Agrotechnologisch Onderzoek, Dienst Landbouwkundig Onderzoek (ATO-DLO) B098) - 43
Houdbaarheids-voorspellend model tomaat : onderzoek naar de relatie tussen de houdbaarheid van partijen tomaten en de stevigheid bij aanvoer op de veiling gedurende de seizoenen 1991 en 1992
Robbers, Jan ; Valk, Henry van der; Tomassen, Monic ; Polderdijk, Anneke ; Wilkinson, C. - \ 1993
Wageningen : Instituut voor Agrotechnologisch Onderzoek ATO-DLO (Rapport / Instituut voor Agrotechnologisch Onderzoek ATO-DLO 346) - 24
Proteolytic enzymes in developing leaves of oats (Avena sativa L.) II. Aminoacyl-2-naphthylamidases.
Valk, H.C.P.M. van der; Bentum, M.I.A. van; Loon, L.C. van - \ 1989
Journal of Plant Physiology 135 (1989). - ISSN 0176-1617 - p. 489 - 494.
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