- A. Pilrczyk (1)
- J.H.W.M. Rombout (1)
- H.F.J. Savelkoul (1)
- J.P. Scharsack (1)
- J.J. Taverne-Thiele (1)
- G.F. Wiegertjes (3)
|Differential contribution of neutrophilic granulocytes and macrophages to nitrosative stress in vivo
Forlenza, M. ; Scharsack, J.P. ; Kachamakova, N.M. ; Taverne-Thiele, J.J. ; Rombout, J.H.W.M. ; Wiegertjes, G.F. - \ 2009
In: 11th International Congress ISDCI, Prague, Czech Republic, 28th June - 4th July, 2009. - - p. 136 - 136.
Genetic differences in natural antibody levels in common carp (Cyprinus carpio L.)
Kachamakova, N.M. ; Irnazarow, I. ; Parmentier, H.K. ; Savelkoul, H.F.J. ; Pilarczyk, A. ; Wiegertjes, G.F. - \ 2006
Fish and Shellfish Immunology 21 (2006)4. - ISSN 1050-4648 - p. 404 - 413.
salmon salmo-salar - disease resistance - immune parameters - igm antibodies - responses - fish - immunoglobulin - protection - innate
In mammals, natural antibodies (Nabs) are mostly of the IgM isotype and can bind to a particular antigen or pathogen even if the host has never been exposed. Despite their early detection and abundance, the exact role and genetic control of Nabs remain unclear. We have used an indirect ELISA with three different antigens (keyhole limpet haemocyanin, chicken ovalbumin and bovine serum albumin) to demonstrate the ubiquitous presence of Nabs in common carp. Serum levels of Nabs increased with age, i.e. 10-month-old fish showed higher levels than 4-month-old fish. Also, fish grown in earth ponds showed higher levels of Nabs than fish grown in a clean environment of UV-treated water. Furthermore, we show that Nabs are present in different levels in the serum of carp lines with a different genetic background, suggestive of a genetic control. These genetic differences were independent of antigen, age and environment. Genetic differences in levels of Nabs could not unequivocally be related to differences in survival under farmed conditions. The possibilities for using levels of Nabs as marker criterion for selection for genetic disease resistance are discussed
|Identification of inducible nitric oxide synthase in eight different carp lines: gene approach for disease resistance
Kachamakova, N.M. ; Wiegertjes, G.F. ; Pilrczyk, A. ; Irnazarow, I. - \ 2003