Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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    Francisella tularensis in Swedish predators and scavengers
    Hestvik, G. ; Uhlhorn, H. ; Koene, M. ; Åkerström, S. ; Malmsten, A. ; Dahl, F. ; Åhlén, P.A. ; Dalin, A.M. ; Gavier-Widén, D. - \ 2019
    Epidemiology and Infection 147 (2019). - ISSN 0950-2688 - p. e293 - e293.
    Agglutination - predator - serology - tularaemia - wildlife

    Tularaemia is a zoonotic disease, in Europe caused by Francisella tularensis subsp. holarctica. Many lagomorphs and a variety of small rodents are wildlife species prone to develop clinical disease, while predators and scavengers are relatively resistant and may serve as sentinels. Blood samples from 656 Swedish wild predators and scavengers were serologically investigated using slide agglutination and microagglutination. In the slide agglutination test, 34 seropositive animals were detected, and they were found among all species investigated: brown bear (Ursus arctos), Eurasian lynx (Lynx lynx), raccoon dog (Nyctereutes procyonoides), red fox (Vulpes vulpes), wild boar (Sus scrofa), wolf (Canis lupus) and wolverine (Gulo gulo). Due to haemolysis the microagglutination test was more difficult to read at low titres, and only 12 animals were classified as seropositive. F. tularensis subsp. holarctica was detected by a polymerase chain reaction in lymphatic tissues of the head in one brown bear, one red fox and one wolf. The significance of this finding regarding possible latency of infection is not clear. In conclusion, the results of this study indicate that all predator and scavenger species included in this study may serve as sentinels for tularaemia in Sweden. Their role as reservoirs is unclear.

    Maternale antilichamen tegen Campylobacter in eieren van verschillende merken kippen
    Koene, M.G.J. ; Goot, J.A. van der; Cornelissen, J.B.W.J. ; Jong, Ingrid de; Kollenstart, Johan ; Hartog, Mark den - \ 2019
    Wageningen Bioveterinary Research
    Phylogeographic distribution of human and hare Francisella tularenses susp. holarctica strains in the Netherlands and its pathology in European brown hares (Lepus europaeus)
    Koene, M.G.J. ; Rijks, Jolianne M. ; Maas, Miriam ; Ruuls, R.C. ; Engelsma, M.Y. ; Tulden, P.W. van; Kik, Marja ; IJzer, Jooske ; Notermans, Daan ; Vries, Maaike de; Fanoy, Ewout ; Pijnacker, Roan ; Spierenburg, Marcel A.H. ; Bavelaar, Herjan ; Berkhout, Hanneke ; Sankatsing, Sanjay ; Diepersloot, Rob ; Myrtennas, Kerstin ; Granberg, Malin ; Forsman, Mats ; Roest, H.I.J. ; Gröne, Andrea - \ 2019
    Frontiers in Cellular and Infection Microbiology 9 (2019). - ISSN 2235-2988 - 11 p.
    Sequence-based typing of Francisella tularensis has led to insights in the evolutionary developments of tularemia. In Europe, two major basal clades of F. tularensis subsp. holarctica exist, with a distinct geographical distribution. Basal clade B.6 is primarily found in Western Europe, while basal clade B.12 occurs predominantly in the central and eastern parts of Europe. There are indications that tularemia is geographically expanding and that strains from the two clades might differ in pathogenicity, with basal clade B.6 strains being potentially more virulent than basal clade B.12. This study provides information on genotypes detected in the Netherlands during 2011–2017. Data are presented for seven autochthonous human cases and for 29 European brown hares (Lepus europaeus) with laboratory confirmed tularemia. Associated disease patterns are described for 25 European brown hares which underwent post-mortem examination. The basal clades B.6 and B.12 are present both in humans and in European brown hares in the Netherlands, with a patchy geographical distribution. For both genotypes the main pathological findings in hares associated with tularemia were severe (sub)acute necrotizing hepatitis and splenitis as well as necrotizing lesions and hemorrhages in several other organs. Pneumonia was significantly more common in the B.6 than in the B.12 cases. In conclusion, the two major basal clades present in different parts in Europe are both present in the Netherlands. In hares found dead, both genotypes were associated with severe acute disease affecting multiple organs. Hepatitis and splenitis were common pathological findings in hares infected with either genotype, but pneumonia occurred significantly more frequently in hares infected with the B.6 genotype compared to hares infected with the B.12 genotype.
    Erratum to “Investigation of Clostridium botulinum group III's mobilome content”
    Woudstra, Cédric ; Maréchal, Caroline Le; Souillard, Rozenn ; Anniballi, Fabrizio ; Auricchio, Bruna ; Bano, Luca ; Bayon-Auboyer, Marie Hélène ; Koene, Miriam ; Mermoud, Isabelle ; Brito, Roseane B. ; Lobato, Francisco C.F. ; Silva, Rodrigo O.S. ; Dorner, Martin B. ; Fach, Patrick - \ 2019
    Anaerobe 57 (2019). - ISSN 1075-9964 - p. 117 - 117.
    Francisella tularensis isolated from human clinical infections
    Koene, M.G.J. ; Rijks, Jolianne M. ; Maas, Miriam ; Ruuls, R.C. ; Engelsma, M.Y. ; Tulden, P.W. van; Kik, Marja ; IJzer, Jooske ; Notermans, Daan ; Vries, Maaike de; Fanoy, Ewout ; Pijnacker, Roan ; Spierenburg, Marcel A.H. ; Bavelaar, Herjan ; Berkhout, Hanneke ; Sankatsing, Sanjay ; Diepersloot, Rob ; Myrtennas, Kerstin ; Granberg, Malin ; Forsman, Mats ; Roest, H.I.J. ; Gröne, Andrea - \ 2018
    Wageningen UR
    PRJEB27514 - PRJEB27514 - ERP109601 - Francisella tularensis
    Genome sequences of Francisella tularensis samples, isolated from human infections in the Netherlands in 2015 and 2016.
    Towards a safe and sustainable poultry production chain
    Berghout, Jacqueline ; Roland, Wibke ; Vollebregt, Martijntje ; Koene, Miriam ; Jong, Ingrid de - \ 2018
    Wageningen : Wageningen Livestock Research (Wageningen Livestock Research report 1126) - 79
    Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015
    Maas, Miriam ; Vries, Ankje De; Reusken, Chantal ; Buijs, Jan ; Goris, Marga ; Hartskeerl, Rudy ; Ahmed, Ahmed ; Tulden, Peter van; Swart, Arno ; Pijnacker, Roan ; Koene, Miriam ; Lundkvist, Åke ; Heyman, Paul ; Rockx, Barry ; Giessen, Joke Van Der - \ 2018
    Infection Ecology and Epidemiology 8 (2018)1. - ISSN 2000-8686
    epidemiology - hantavirus - Leptospirosis - prevalence - Rattus norvegicus - Seoul virus

    Background: Brown rats (Rattus norvegicus) may carry pathogens that can be a risk for public health. Brown rats in the Netherlands were tested for the zoonotic pathogens Leptospira spp. and Seoul hantavirus (SEOV), in order to obtain insight in their prevalence. Methods and results: Cross-sectional studies were performed at four locations from 2011 to 2015. The rats were tested for Leptospira spp. using real-time PCR and/or culture resulting in a prevalence ranging between 33–57%. Testing for SEOV was done through an adapted human Seoul hantavirus ELISA and real-time RT-PCR. Although at several locations the ELISA indicated presence of SEOV antibodies, none could be confirmed by focus reduction neutralization testing. Conclusion: The results indicate a widespread presence of Leptospira spp. in brown rats in the Netherlands, including areas with a low leptospirosis incidence in humans. No evidence for circulation of SEOV was found in this study.

    Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands
    Kroese, Michiel V. ; Beckers, Lisa ; Bisselink, Yvette J.W.M. ; Brasseur, Sophie ; Tulden, Peter W. van; Koene, Miriam G.J. ; Roest, Hendrik I.J. ; Ruuls, Robin C. ; Backer, Jantien A. ; Ijzer, Jooske ; Giessen, Joke W.B. van der; Willemsen, Peter T.J. - \ 2018
    Journal of Wildlife Diseases 54 (2018)3. - ISSN 0090-3558 - p. 439 - 449.
    Brucella pinnipedialis - Halichoerus grypus - MALDI-TOF MS - Marine mammals - MLST - MLVA-16 - Phoca vitulina - The Netherlands

    Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.

    Chicken immune response following in ovo delivery of bacterial flagellin
    Vaezirad, M.M. ; Koene, M.G. ; Wagenaar, J.A. ; Putten, J.P.M. van - \ 2018
    Vaccine 36 (2018)16. - ISSN 0264-410X - p. 2139 - 2146.
    Chicken - Flagellin - Humoral Response - In ovo immunization - Toll-like receptor - Vaccine
    In ovo immunization of chicken embryos with live vaccines is an effective strategy to protect chickens against several viral pathogens. We investigated the immune response of chicken embryos to purified recombinant protein. In ovo delivery of Salmonella flagellin to 18-day old embryonated eggs resulted in elevated pro-inflammatory chIL-6 and chIL-8 (CXCL8-CXCLi2) cytokine transcript levels in the intestine but not in the spleen at 24 h post-injection. Analysis of the chicken Toll-like receptor (TLR) repertoire in 19-day old embryos revealed gene transcripts in intestinal and spleen tissue for most chicken TLRs, including TLR5 which recognizes Salmonella flagellin (FliC). The in ovo administration of FliC did not alter TLR transcript levels, except for an increase in intestinal chTLR15 expression. Measurement of the antibody response in sera collected at day 11 and day 21 post-hatch demonstrated high titers of FliC-specific antibodies for the animals immunized at the late-embryonic stage in contrast to the mock-treated controls. The successful in ovo immunization with purified bacterial antigen indicates that the immune system of the chicken embryo is sufficiently mature to yield a strong humoral immune response after single exposure to purified protein. This finding strengthens the basis for the development of in ovo protein-based subunit vaccines.
    Investigation of Clostridium botulinum group III's mobilome content
    Woudstra, Cédric ; Maréchal, Caroline Le; Souillard, Rozenn ; Anniballi, Fabrizio ; Auricchio, Bruna ; Bano, Luca ; Bayon-Auboyer, Marie Hélène ; Koene, Miriam ; Mermoud, Isabelle ; Brito, Roseane B. ; Lobato, Francisco C.F. ; Silva, Rodrigo O.S. ; Dorner, Martin B. ; Fach, Patrick - \ 2018
    Anaerobe 49 (2018). - ISSN 1075-9964 - p. 71 - 77.
    Animal botulism - Clostridium botulinum group III - Phage - Plasmid
    Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.
    Relation between soiling of live broilers and Campylobacter levels during the slaughtering process
    Koene, M.G.J. ; Jong, I.C. de; Goot, Jeanet van der; Wagenaar, J.A. ; Hartog, M. den - \ 2017
    In: 19th International workshop on Campylobacter Heliobacter and related organisms. - - p. 71 - 71.
    Campylobacter status of different broiler concepts in the Netherlands
    Koene, M.G.J. ; Jong, I.C. de; Goot, Jeanet van der; Wagenaar, J.A. ; Hartog, M. den - \ 2017
    In: 19th International workshop on Campylobacter, Helicobacter and related organisms. - - p. 320 - 320.
    Campylobacter colonization in broiler raised under different management concepts
    Koene, M.G.J. ; Jong, I.C. de; Goot, J.A. van der; Wagenaar, J.A. ; Hartog, M. den - \ 2017
    Botulisme bij een Poolse arbeider in Zeist
    Hintaran, A.D. ; Pruissen, F.G.M. van; Stam, A.J. ; Engelsma, M.Y. ; Janse, L. ; Fanoy, E. ; Koene, M.G.J. - \ 2017
    Tijdschrift voor Infectieziekten 12 (2017)3. - ISSN 1872-0811 - p. 84 - 87.
    Toepassing van Undine in Nederlandse vleeskuikenslachterijen : Onderzoek naar het effect van Undine technologie op Campylobacter niveaus op borstvel
    Koene, M.G.J. ; Goot, J.A. van der; Hartog, M.L. den - \ 2017
    Central Veterinary Institute, onderdeel van Wageningen UR
    First case of severe pneumonic tularemia in an immunocompetent patient in the Netherlands
    Sigaloff, K.C.E. ; Chung, P.K. ; Koopmans, J. ; Notermans, D.W. ; Rijckevorsel, G.G.C. Van; Koene, M. ; Sprengers, R.W. ; Gooskens, J. ; Stalenhoef, J.E. - \ 2017
    The Netherlands Journal of Medicine 75 (2017)7. - ISSN 0300-2977 - p. 301 - 303.
    Francisella tularensis - Pneumonia - Tularemia

    Tularemia is a zoonosis caused by different subspecies of the Gram-negative bacterium Francisella tularensis. We report the first case in the Netherlands of pneumonic tularemia caused by the F. tularensis subspecies holarctica after probable occupational inhalation of contaminated aerosols. Notification of cases of tularemia has been mandatory by law in the Netherlands since 1 November 2016.

    Environmental surveillance during an outbreak of tularaemia in hares, the Netherlands, 2015
    Janse, Ingmar ; Maas, M. ; Rijks, J.M. ; Koene, M. ; Plaats, R.Q. van der; Engelsma, M. ; Tas, P.W.L. ; Braks, M. ; Stroo, A. ; Notermans, D.W. ; Vries, M.C. de; Reubsaet, F.A.G. ; Fanoy, E. ; Swaan, C.M. ; Kik, M.J. ; Ijzer, J. ; Jaarsma, R.I. ; Wieren, S. van; Roda Husman, A.M. de; Passel, M. van; Roest, H. ; Giessen, J. van der - \ 2017
    Eurosurveillance 22 (2017)35. - ISSN 1025-496X
    Tularaemia, a disease caused by the bacterium Francisella tularensis, is a re-emerging zoonosis in the Netherlands. After sporadic human and hare cases occurred in the period 2011 to 2014, a cluster of F. tularensis-infected hares was recognised in a region in the north of the Netherlands from February to May 2015. No human cases were identified, including after active case finding. Presence of F. tularensis was investigated in potential reservoirs and transmission routes, including common voles, arthropod vectors and surface waters. F. tularensis was not detected in common voles, mosquito larvae or adults, tabanids or ticks. However, the bacterium was detected in water and sediment samples collected in a limited geographical area where infected hares had also been found. These results demonstrate that water monitoring could provide valuable information regarding F. tularensis spread and persistence, and should be used in addition to disease surveillance in wildlife.
    Evaluation of a multiplex real-time PCR for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid
    Wisselink, H.J. ; Cornelissen, J.B.W.J. ; Wal, F.J. van der; Kooi, Bart ; Koene, M.G.J. ; Bossers, A. ; Smid, B. ; Bree, F.M. de; Antonis, A.F.G. - \ 2017
    BMC Veterinary Research 13 (2017)1. - ISSN 1746-6148
    Pasteurella multocida, Mannheimia haemolytica, Histophilus somni and Trueperella pyogenes are four bacterial agents commonly associated with bovine respiratory disease (BRD). In this study a bacterial multiplex real-time PCR (the RespoCheck PCR) was evaluated for the detection in bronchoalveolar lavage fluid (BALF) of these four bacterial agents.
    The analytical sensitivity of the multiplex real-time PCR assay determined on purified DNA and on bacterial cells of the four target pathogens was one to ten fg DNA/assay and 4 × 10−1 to 2 × 100 CFU/assay. The analytical specificity of the test was, as evaluated on a collection of 118 bacterial isolates, 98.3% for M. haemolytica and 100% for the other three target bacteria. A set of 160 BALF samples of calves originating from ten different herds with health problems related to BRD was examined with bacteriological methods and with the RespoCheck PCR. Using bacteriological examination as the gold standard, the diagnostic sensitivities and specificities of the four bacterial agents were respectively between 0.72 and 1.00 and between 0.70 and 0.99. Kappa values for agreement between results of bacteriological examination and PCRs were low for H. somni (0.17), moderate for P. multocida (0.52) and M. haemolytica (0.57), and good for T. pyogenes (0.79). The low and moderate kappa values seemed to be related to limitations of the bacteriological examination, this was especially the case for H. somni.
    It was concluded that the RespoCheck PCR assay is a valuable diagnostic tool for the simultaneous detection of the four bacterial agents in BALF of calves.
    Een jongen met tularemie na een modderrace
    Zijlstra, Marieke ; Hulsker, Caroline C.C. ; Fanoy, Ewout B. ; Pijnacker, Roan ; Kraaijeveld, Arie ; Koene, Miriam G.J. ; Wolfs, Tom F.W. - \ 2017
    Nederlands Tijdschrift voor Geneeskunde 161 (2017)23. - ISSN 0028-2162

    Background Tularaemia is a rare disease. In Europe it mostly occurs in Scandinavia. Since 2011 more cases are being reported in the Netherlands. Tularaemia may manifest itself in various ways. It is important to take strict precautions during biopsy, drainage and biopsy processing in order to prevent transmission. Case description A 10yearold boy presented to the paediatrician with a left inguinal lymphadenitis. A week before the onset of symptoms he had participated in a children's mud race. Serology and PCR of pus from the lymph node tested positive for Francisella tularensis. Treatment with ciprofloxacin was insufficiently effective, so surgical drainage of the gland was performed under strict isolation conditions. Water from the mud race location contained genetic material from F. tularensis. Conclusion Given the rising incidence of tularaemia in the Netherlands, it is important to consider 'tularaemia' in the differential diagnosis in patients with lymphadenitis and epidemiological clues in their case history. Since 1 November 2016 it has been mandatory to report tularaemia in the Netherlands.

    Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases
    Cornelissen, Jan B.W.J. ; Bree, Freddy M. de; Wal, Fimme J. van der; Kooi, Engbert A. ; Koene, Miriam G.J. ; Bossers, Alex ; Smid, Bregtje ; Antonis, Adriaan F. ; Wisselink, Henk J. - \ 2017
    BMC Veterinary Research 13 (2017)1. - ISSN 1746-6148
    Bovine Mycoplasma - Bovine respiratory disease - M. bovirhinis - M. bovis - M. dispar - RespoCheck - Triplex PCR

    Background: In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCheck Mycoplasma triplex real-time PCR are based on the V3/V4 region of the 16S rRNA gene of the three Mycoplasma species. Results: The analytical sensitivity of the RespoCheck triplex real-time PCR was, as determined by spiking experiments of the Mycoplasma strains in Phosphate Buffered Saline, 300 colony forming units (cfu)/mL for M. dispar, and 30cfu/mL for M. bovis or M. bovirhinis. The analytical sensitivity of the RespoCheck Mycoplasma triplex real-time PCRwas, as determined on purified DNA, 10fg DNA per assay for M. dispar and 100fg fo rM. bovis and M. bovirhinis. The analytical specificity of the RespoCheck Mycoplasma triplex real-time PCR was, as determined by testing Mycoplasmas strains (n=17) and other bacterial strains (n=107), 100, 98.2 and 99.1% for M. bovis, M. dispar and M. bovirhinis respectively. The RespoCheck Mycoplasma triplex real-time PCR was compared with the PCR/DGGE analysis for M. bovis, M. dispar and M. bovirhinis respectively by testing 44 BALF samples from calves. Conclusion: In conclusion, the RespoCheck PCR assay can be a valuable tool for timely and accurate detection of three Mycoplasma species associated with in bovine respiratory disease.

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