- T. Charnikhova (1)
- P. Christou (1)
- L. Colombo (2)
- L. Dreni (1)
- G. Droc (1)
- L.J.G. Enckevort van (1)
- C. Favalli (1)
- F. Fornara (1)
- C. Gagneur (1)
- V.M. Gonzalez (1)
- R. Greco (4)
- E. Guiderdoni (3)
- J. Heijmans (1)
- J.H.C. Hoge (2)
- A. Hoogenboom (1)
- L. Jiang (1)
- R.J. Kam de (1)
- M.M. Kater (1)
- W. Kohlen (1)
- P. Lan (1)
- C.M. Liu (1)
- A.H. Meijer (4)
- C. Michel (1)
- B. Miro (1)
- J.B. Morel (1)
- P.P.J. Mulder (1)
- P.B.F. Ouwerkerk (6)
- E. Pe' (1)
- A.B. Pereira (2)
- A. Pereira (1)
- P. Piffanelli (1)
- P. Puigdomenech (1)
- M. Rafel (1)
- C. Sallaud (2)
- H. Schluepmann (1)
- A.J.C. Taal (1)
- B. Wang (1)
- C. Weber (1)
- Y. Xiao (1)
- S. Zhang (1)
- Z. Zhu (1)
OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice
Xiao, Y. ; Charnikhova, T. ; Mulder, P.P.J. ; Heijmans, J. ; Hoogenboom, A. ; Agalou, A. ; Michel, C. ; Morel, J.B. ; Dreni, L. ; Kater, M.M. ; Bouwmeester, H.J. ; Wang, B. ; Zhu, Z. ; Ouwerkerk, P.B.F. - \ 2014
Plant Molecular Biology 86 (2014)1-2. - ISSN 0167-4412 - p. 19 - 33.
jasmonic acid biosynthesis - male-sterile mutant - l-isoleucine - methyl jasmonate - gene encodes - arabidopsis - enzyme - proteins - tomato - (+)-7-iso-jasmonoyl-l-isoleucine
Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice
Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice
Zhang, S. ; Kohlen, W. ; Jiang, L. ; Bouwmeester, H.J. ; Meijer, A.H. ; Schluepmann, H. ; Liu, C.M. ; Ouwerkerk, P.B.F. - \ 2012
Plant Molecular Biology 80 (2012)6. - ISSN 0167-4412 - p. 571 - 585.
plant craterostigma-plantagineum - acid signal-transduction - length cdna microarray - high-salinity stresses - abscisic-acid - transcription factor - homeobox gene - water-deficit - arabidopsis-thaliana - dna-binding
Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors, most of which have unknown functions. Here we show that the expression of Oshox22 is strongly induced by salt stress, abscisic acid (ABA), and polyethylene glycol treatment (PEG), and weakly by cold stress. Trans-activation assays in yeast and transient expression analyses in rice protoplasts demonstrated that Oshox22 is able to bind the CAAT(G/C)ATTG element and acts as a transcriptional activator that requires both the HD and Zip domains. Rice plants homozygous for a T-DNA insertion in the promoter region of Oshox22 showed reduced Oshox22 expression and ABA content, decreased sensitivity to ABA, and enhanced tolerance to drought and salt stresses at the seedling stage. In contrast, transgenic rice over-expressing Oshox22 showed increased sensitivity to ABA, increased ABA content, and decreased drought and salt tolerances. Based on these results, we conclude that Oshox22 affects ABA biosynthesis and regulates drought and salt responses through ABA-mediated signal transduction pathways.
Suppression of an atypically spliced rice CACTA transposon transcript in transgenic plants
Greco, R. ; Ouwerkerk, P.B.F. ; Pereira, A.B. - \ 2005
Genetics 169 (2005)4. - ISSN 0016-6731 - p. 2383 - 2387.
zea-mays - element system - gene - insertion - genome - retrotransposons - arabidopsis - protein - intron - tnpa
OsES1, a rice homolog of the maize En/Spm transposon, is transcribed to produce TnpA-like and TnpD-like transcripts. However, an alternatively spliced form of the TnpA-like transcript., which was found to be suppressed in transgenic plants, was revealed to be clue to atypical splicing of a Hipa-like CACTA transposon.
EU-OSTID: A collection of transposon insertional mutants for functional genomics in rice
Enckevort, L.J.G. van; Droc, G. ; Piffanelli, P. ; Greco, R. ; Gagneur, C. ; Weber, C. ; Gonzalez, V.M. ; Cabot, P. ; Fornara, F. ; Berri, S. ; Miro, B. ; Lan, P. ; Rafel, M. ; Capell, T. ; Puigdomenech, P. ; Ouwerkerk, P.B.F. ; Meijer, A.H. ; Pe', E. ; Colombo, L. ; Christou, P. ; Guiderdoni, E. - \ 2005
Plant Molecular Biology 59 (2005)1. - ISSN 0167-4412 - p. 99 - 110.
reverse genetics - homeobox genes - mutagenesis - expression - system - family - annotation - generation - resources - activator
A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.
Transcription and somatic transposition of the maize En/Spm transposon system in rice
Greco, R. ; Ouwerkerk, P.B.F. ; Taal, A.J.C. ; Sallaud, C. ; Guiderdoni, E. ; Meijer, A.H. ; Hoge, J.H.C. ; Pereira, A.B. - \ 2004
Molecular Genetics and Genomics 270 (2004)6. - ISSN 1617-4615 - p. 514 - 523.
dna insertional mutagenesis - suppressor-mutator element - zea-mays - functional genomics - molecular analysis - transgenic tobacco - arabidopsis-thaliana - spm element - en spm - trapping system
Transposition of the maize En/Spm system in rice was investigated using a two-component construct consisting of an immobilised transposase source driven by the CaMV 35S-promoter, and a modified I/dSpm transposon. Mobilization of I/dSpm in somatic sectors was demonstrated by sequencing of excision products and isolation of flanking genomic sequences in T0 and T1 progeny plants. Since the transposition efficiency appeared to be considerably lower than that observed in maize or in other heterologous systems like Arabidopsis, we examined En/Spm transcription and splicing in the transgenic rice plants. Northern analysis revealed the presence of transcripts encoding the active TnpA and TnpD transposases, with the latter predominating; this is the reverse of what is seen in maize and Arabidopsis. RT-PCR analysis confirmed the occurrence of correct splicing and the formation of the two other alternatively spliced transcripts (TnpB and TnpC), as previously described for maize. Two alternative splice donor sites at the end of exon 1 were identified in maize at positions 578 and 704. We observe that rice is similar to maize in that TnpA is preferentially spliced at position 578. We also show that in Arabidopsis splicing occurs preferentially at position 704, as in other dicots like tobacco. These observations indicate differences in the splicing of transcripts of the maize En/Spm element between dicot and monocot hosts. Nevertheless, the ratio in which the transcripts for the active transposases are produced seems to determine the efficiency of transposition, irrespective of the host considered. A limiting amount of TnpA might therefore be responsible for the lower transposition activity of En/Spm in rice. Alternatively, reduced mobility of the modified I/dSpm element used may have resulted from the absence of critical sequences necessary for transposition. The influence of endogenous, autonomous, En/Spm -related elements present in the rice genome on the transposition behaviour of the exogenous maize element is also considered
Transpositional behaviour of an Ac/Ds system for reverse genetics in rice
Greco, R. ; Ouwerkerk, P.B.F. ; Kam, R.J. de; Sallaud, C. ; Favalli, C. ; Colombo, L. ; Guiderdoni, E. ; Meijer, A.H. ; Hoge, J.H.C. ; Pereira, A. - \ 2003
Theoretical and Applied Genetics 108 (2003)1. - ISSN 0040-5752 - p. 10 - 24.
site-specific recombination - dna insertional mutagenesis - transposase messenger-rna - functional genomics - t-dna - tissue-culture - cre-lox - dissociation excision - methylation pattern - controlling element
A collection of transposon Ac/Ds enhancer trap lines is being developed in rice that will contribute to the development of a rice mutation machine for the functional analysis of rice genes. Molecular analyses revealed high transpositional activity in early generations, with 62% of the T0 primary transformants and more than 90% of their T1 progeny lines showing ongoing active transposition. About 10% of the lines displayed amplification of the Ds copy number. However, inactivation of Ds seemed to occur in about 70% of the T2 families and in the T3 generation. Southern blot analyses revealed a high frequency of germinal insertions inherited in the T1 progeny plants, and transmitted preferentially over the many other somatic inserts to later generations. The sequencing of Ds flanking sites in subsets of T1 plants indicated the independence of insertions in different T1 families originating from the same T0 line. Almost 80% of the insertion sites isolated showing homology to the sequenced genome, resided in genes or within a range at which neighbouring genes could be revealed by enhancer trapping. A strategy involving the propagation of a large number of T0 and T1 independent lines is being pursued to ensure the recovery of a maximum number of independent insertions in later generations. The inactive T2 and T3 lines produced will then provide a collection of stable insertions to be used in reverse genetics experiments. The preferential insertion of Ds in gene-rich regions and the use of lines containing multiple Ds transposons will enable the production of a large population of inserts in a smaller number of plants. Additional features provided by the presence of lox sites for site-specific recombination, or the use of different transposase sources and selectable markers, are discussed