Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Selective deoxygenation of stearic acid via an anhydride pathway
Hollak, S.A.W. ; Bitter, W. ; Haveren, J. van; Es, D.S. van - \ 2012
RSC Advances : An international journal to further the chemical sciences 2 (2012)25. - ISSN 2046-2069 - p. 9387 - 9391.
diesel-like fuel - catalytic deoxygenation - vegetable-oils - carboxylic-acids - fatty-acids - mesoporous carbon - renewable diesel - pd/c catalyst - pyrolysis - decarboxylation
Stearic anhydride is proposed as reactive intermediate in the hydrogen free decarbonylation and ketonization of stearic acid over Pd/Al2O3 at 523 K. This information is crucial towards developing of a selective low temperature decarbonylation process of fatty acids towards olefins.
ESX-1-mediated translocation to the cytosol controls virulence of mycobacteria
Houben, D. ; Demangel, C. ; Ingen, J. van; Perez, J. ; Baldeon, L. ; Abdallah, A.M. ; Caleechurn, L. ; Bottal, D. ; Zon, M. van; Punder, K. de; Laan, T. van der; Kant, A. ; Willemsen, P. ; Bitter, W. ; Soolingen, D. ; Brosch, R. ; Wel, N. van der - \ 2012
Cellular Microbiology 14 (2012)8. - ISSN 1462-5814 - p. 1287 - 1298.
t-cell antigen - listeria-monocytogenes - alveolar macrophages - phagosomal membranes - clinical-relevance - phospholipases-c - vii secretion - bovis bcg - tuberculosis - leprae
Mycobacterium species, including Mycobacterium tuberculosis and Mycobacterium leprae, are among the most potent human bacterial pathogens. The discovery of cytosolic mycobacteria challenged the paradigm that these pathogens exclusively localize within the phagosome of host cells. As yet the biological relevance of mycobacterial translocation to the cytosol remained unclear. In this current study we used electron microscopy techniques to establish a clear link between translocation and mycobacterial virulence. Pathogenic, patient-derived mycobacteria species were found to translocate to the cytosol, while non-pathogenic species did not. We were further able to link cytosolic translocation with pathogenicity by introducing the ESX-1 (type VII) secretion system into the non-virulent, exclusively phagolysosomal Mycobacterium bovis BCG. Furthermore, we show that translocation is dependent on the C-terminus of the early-secreted antigen ESAT-6. The C-terminal truncation of ESAT-6 was shown to result in attenuation in mice, again linking translocation to virulence. Together, these data demonstrate the molecular mechanism facilitating translocation of mycobacteria. The ability to translocate from the phagolysosome to the cytosol is with this study proven to be biologically significant as it determines mycobacterial virulence.
The mannose cap of mycobacterial lipoarabinomannan does not dominate the Mycobacterium–host interaction
Appelmelk, B.J. ; Dunnen, J. ; Driessen, N.N. ; Ummels, R. ; Pak, M. ; Nigou, J. ; Larrouy-Maumus, G. ; Gurcha, S.S. ; Movahedzadeh, F. ; Geurtsen, J. ; Brown, E.J. ; Smeets, M.M.E. ; Besra, G.S. ; Willemsen, P.T.J. ; Lowary, T.L. ; Kooyk, Y. van; Maaskant, J.J. ; Stoker, N.G. ; Ley, P. de; Puzo, G. ; Vandenbroucke-Grauls, C.M.J.E. ; Wieland, C.W. ; Poll, T. van der; Geijtenbeek, T.B.H. ; Sar, A.M. van der; Bitter, W. - \ 2008
Cellular Microbiology 10 (2008)4. - ISSN 1462-5814 - p. 930 - 944.
human dendritic cells - dc-sign - arabinan biosynthesis - intracellular survival - transferase-activity - tuberculosis - receptor - binding - galactofuranosyltransferase - mannosyltransferases
Pathogenic mycobacteria have the ability to persist in phagocytic cells and to suppress the immune system. The glycolipid lipoarabinomannan (LAM), in particular its mannose cap, has been shown to inhibit phagolysosome fusion and to induce immunosuppressive IL-10 production via interaction with the mannose receptor or DC-SIGN. Hence, the current paradigm is that the mannose cap of LAM is a crucial factor in mycobacterial virulence. However, the above studies were performed with purified LAM, never with live bacteria. Here we evaluate the biological properties of capless mutants of Mycobacterium marinum and M. bovis BCG, made by inactivating homologues of Rv1635c. We show that its gene product is an undecaprenyl phosphomannose-dependent mannosyltransferase. Compared with parent strain, capless M. marinum induced slightly less uptake by and slightly more phagolysosome fusion in infected macrophages but this did not lead to decreased survival of the bacteria in vitro, nor in vivo in zebra fish. Loss of caps in M. bovis BCG resulted in a sometimes decreased binding to human dendritic cells or DC-SIGN-transfected Raji cells, but no differences in IL-10 induction were observed. In mice, capless M. bovis BCG did not survive less well in lung, spleen or liver and induced a similar cytokine profile. Our data contradict the current paradigm and demonstrate that mannose-capped LAM does not dominate the Mycobacterium-host interaction.
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