Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle
Kaba, S.A. ; Musoke, A.J. ; Schaap, D. ; Schetters, T. ; Rowlands, J. ; Vermeulen, A.J. ; Nene, V. ; Vlak, J.M. ; Oers, M.M. van - \ 2005
Vaccine 23 (2005)21. - ISSN 0264-410X - p. 2791 - 2800.
theileria-parva sporozoites - neutralizing epitopes - monoclonal-antibody - surface-antigen - protection - protein - stocks - immunogenicity - immunization - leukocytes
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67¿SS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67¿SS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 ¿g per dose, which is a major improvement over previous recombinant p67 products
Intervet Symposium: bovine neosporosis
Schetters, T. ; Dubey, J.P. ; Adrianarivo, A. ; Frankena, K. ; Romero, J.J. ; Pérez, E. ; Heuer, C. ; Nicholson, C. ; Russell, D. ; Weston, J. - \ 2004
Veterinary Parasitology 125 (2004)1-2. - ISSN 0304-4017 - p. 137 - 146.
caninum-associated abortion - dairy-cattle - vertical transmission - immune-responses - infection - herds - dogs - cows - protection - california
This article summarises the most relevant data of presentations delivered at the 19th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP) held in New Orleans, LA, USA, from 10 to 14 August 2003) in a symposium session on bovine neosporosis. The symposium was organised by Juan Munoz-Bielsa, Wicher Holland, Enzo Foccoli and Theo Schetters (chairman). The focus was on the present state of knowledge of the biology, epidemiology (presented by J.P. Dubey) and immunology of Neospora infection (presented by A. Adrianarivo), with special emphasis on the prospects of vaccination of cattle against Neospora-induced abortion (presentations of K. Frankena (Costa Rican trial) and C. Heuer (New Zealand trial)).