Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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The Effects of Repeated Exposure to Graphic Fear Appeals on Cigarette Packages: A Field Experiment
Dijkstra, A. ; Bos, C. - \ 2015
Psychology of Addictive Behaviors 29 (2015)1. - ISSN 0893-164X - p. 82 - 90.
public-health campaigns - warning labels - smoking-cessation - disengagement beliefs - behavior-change - smokers - impact - messages - metaanalysis - adolescents
Experimental studies on the effects of graphic fear appeals on cigarette packages typically expose smokers in a single session to a fear appeal, although in practice the exposure is always repeated. The present study applied an improved study design with repeated exposure to fear appeals on cigarette packages. In this field-experiment, 118 smokers were assigned to 1 of 2 conditions with either graphic fear appeals or textual warnings on their cigarette packages. During 3 weeks, fear and disgust were assessed 6 times. The intention to quit smoking after 3 weeks and quitting activity during the 3 weeks were the dependent measures. The effects of 3 pretest individual difference moderators were tested: disengagement beliefs, number of cigarettes smoked a day, and readiness to quit. Three weeks of exposure to the graphic fear appeals led to a stronger intention to quit, but only when smokers scored low on disengagement beliefs, or were heavier smokers. In addition, smokers low in disengagement more often reported to have cut down on smoking in the graphic condition. There were no indications of habituation of fear and disgust over the 3 weeks. The effects of graphic fear appeals depended on smokers' characteristics: The moderators may explain the mixed findings in the literature. The lack of habituation may be caused by the renewal of the graphics every few days. The used field-experimental design with natural repeated exposure to graphics is promising.
Vibrio vulnificus outbreaks in Dutch eel farms since 1996: strain diversity and impact.
Haenen, O.L.M. ; Zanten, E. van; Jansen, R. ; Roozenburg, I. ; Engelsma, M.Y. ; Dijkstra, A. ; Boers, S.A. ; Voorbergen-Laarman, M. ; Möller, A.V.M. - \ 2014
Diseases of Aquatic Organisms 108 (2014)3. - ISSN 0177-5103 - p. 201 - 209.
diseased european eel - anguilla-anguilla - biotype 2 - clinical-manifestations - fresh-water - serovar-e - infection - pathogen - tilapia - fish
Vibrio vulnificus is a potentially zoonotic bacterial pathogen of fish, which can infect humans (causing necrotic fasciitis). We analysed 24 V. vulnificus isolates (from 23 severe eel disease outbreaks in 8 Dutch eel farms during 1996 to 2009, and 1 clinical strain from an eel farmer) for genetic correlation and zoonotic potential. Strains were typed using biotyping and molecular typing by high-throughput multilocus sequence typing (hiMLST) and REP-PCR (Diversilab®). We identified 19 strains of biotype 1 and 5 of biotype 2 (4 from eels, 1 from the eel farmer), that were subdivided into 8 MLST types (ST) according to the international standard method. This is the first report of V. vulnificus biotype 1 outbreaks in Dutch eel farms. Seven of the 8 STs, of unknown zoonotic potential, were newly identified and were deposited in the MLST database. The REP-PCR and the MLST were highly concordant, indicating that the REP-PCR is a useful alternative for MLST. The strains isolated from the farmer and his eels were ST 112, a known potential zoonotic strain. Antimicrobial resistance to cefoxitin was found in most of the V. vulnificus strains, and an increasing resistance to quinolones, trimethoprim + sulphonamide and tetracycline was found over time in strain ST 140. Virulence testing of isolates from diseased eels is recommended, and medical practitioners should be informed about the potential risk of zoonotic infections by V. vulnificus from eels for the prevention of infection especially among high-risk individuals. Additional use of molecular typing methods such as hiMLST and Diversilab® is recommended for epidemiological purposes during V. vulnificus outbreaks.
Factors influencing assessment quality in higher vocational education
Baartman, L. ; Gulikers, J.T.M. ; Dijkstra, A. - \ 2013
Assessment & Evaluation in Higher Education 38 (2013)8. - ISSN 0260-2938 - p. 978 - 997.
assessing professional competence - argument-based approach - validity - validation - performance
The development of assessments that are fit to assess professional competence in higher vocational education requires a reconsideration of assessment methods, quality criteria and (self)evaluation. This article examines the self-evaluations of nine courses of a large higher vocational education institute. Per course, 4–11 teachers and 3–10 students participated. The purpose of this article is to critically examine the quality of assessment in higher vocational education, to identify critical factors influencing assessment quality and to study whether self-evaluation leads to concrete points for improvement. Results show that strong points are fitness for purpose, comparability and fairness. Weak points are reproducibility of decisions and development of self-regulated learning. Critical factors are the translation of competences into assessment criteria to be used in daily lessons and the involvement of the work field. The self-evaluations generated many points for improvement, but not all were translated into actions. Altogether, this article provides a rich picture of assessment quality in higher education and identifies quality aspects that need improvement, (partly) confirming other research on current assessment methods.
Vibrio vulnificus outbreaks in Dutch eel culture: molecular genotyping, antibiotic resistance and zoonotic impact
Haenen, O.L.M. ; Zanten, E. van; Jansen, R. ; Roozenburg-Hengst, R.E.M. ; Dijkstra, A. ; Boers, S.A. ; Voorbergen-Laarman, H.A. ; Möller, A.V.M. - \ 2013
Vibrio vulnificus outbreaks in Dutch eel culture: molecular genotyping, antibiotic resistance and zoonotic impact
Haenen, O.L.M. ; Zanten, E. van; Jansen, R. ; Roozenburg-Hengst, R.E.M. ; Engelsma, M.Y. ; Dijkstra, A. ; Boers, S.A. ; Voorbergen-Laarman, H.A. ; Möller, A.V.M. - \ 2013
Vibrio vulnificus outbreaks in Dutch eel culture: molecular genotyping, antibiotic resistance and zoonotic impact
Haenen, O.L.M. ; Zanten, E. van; Jansen, R. ; Roozenburg-Hengst, R.E.M. ; Engelsma, M.Y. ; Dijkstra, A. ; Boers, S.A. ; Voorbergen-Laarman, H.A. ; Möller, A.V.M. - \ 2013
Aantallen en verspreiding van Eiders en Toppers in de Waddenzee in december 2012
Smit, C.J. ; Jong, M.L. de; Dijkstra, A. - \ 2013
Den Burg : IMARES (Rapport / IMARES Wageningen UR C152/13) - 24
watervogels - eenden - monitoring - winter - waddenzee - waterfowl - ducks - wadden sea
Tellingen van duikeenden in de Waddenzee worden in principe één maal per jaar in het winterseizoen uitgevoerd. Tevens zijn door Alterra/IMARES in de jaren 2008 t/m 2010 aanvullende tellingen uitgevoerd in november, december en februari. Het blijkt niet altijd mogelijk om tellingen in januari te realiseren. Vanwege de onzekerheid om in alle jaren in januari een telling van duikeenden in de Waddenzee te kunnen uitvoeren, is in verschillende rapportages geadviseerd om in de winter een tweede telling uit te voeren, naast de reguliere januari-telling. Deze zouden bij voorkeur moeten worden uitgevoerd in december omdat in deze maand de in de Waddenzee aanwezige aantallen het meest overeenkomen met die in januari.
Effects of bite size and duration of oral processing on retro-nasal aroma release - features contributing to meal termination
Ruijschop, R.M.A.J. ; Zijlstra, N. ; Boelrijk, A.E.M. ; Dijkstra, A. ; Burgering, M.J.M. ; Graaf, C. de; Westerterp-Plantenga, M.S. - \ 2011
The British journal of nutrition 105 (2011)2. - ISSN 0007-1145 - p. 307 - 315.
flavor release - swallowing process - food-intake - perception - time - satiation - desserts - texture - mouth - taste
The brain response to a retro-nasally sensed food odour signals the perception of food and it is suggested to be related to satiation. It is hypothesised that consuming food either in multiple small bite sizes or with a longer durations of oral processing may evoke substantial oral processing per gram consumed and an increase in transit time in the oral cavity. This is expected to result in a higher cumulative retro-nasal aroma stimulation, which in turn may lead to increased feelings of satiation and decreased food intake. Using real-time atmospheric pressure chemical ionisation-MS, in vivo retro-nasal aroma release was assessed for twenty-one young, healthy and normal-weight subjects consuming dark chocolate-flavoured custard. Subjects were exposed to both free or fixed bite size (5 and 15 g) and durations of oral processing before swallowing (3 and 9 s) in a cross-over design. For a fixed amount of dark chocolate-flavoured custard, consumption in multiple small bite sizes resulted in a significantly higher cumulative extent of retro-nasal aroma release per gram consumed compared with a smaller amount of large bite sizes. In addition, a longer duration of oral processing tended to result in a higher cumulative extent of retro-nasal aroma release per gram consumed compared with a short duration of oral processing. An interaction effect of bite size and duration of oral processing was not observed. In conclusion, decreasing bite size or increasing duration of oral processing led to a higher cumulative retro-nasal aroma stimulation per gram consumed. Hence, adapting bite size or duration of oral processing indicates that meal termination can be accelerated by increasing the extent of retro-nasal aroma release and, subsequently, the satiation.
Sobere herinrichting van waterschapswegen werkt; aantal ongevallen daalt met 24 procent (internetartikel)
Jaarsma, C.F. ; Dijkstra, A. ; Louwerse, W.J.R. ; Vries, J.R. de - \ 2011
Verkeerskunde 2011 (2011)6. - ISSN 0377-8495 - 7 p.
Sobere herinrichting van waterschapswegen werkt; aantal ongevallen daalt met 24%
Jaarsma, C.F. ; Dijkstra, A. ; Louwerse, W.J.R. ; Vries, J.R. de - \ 2011
Verkeerskunde 2011 (2011)62. - ISSN 0377-8495 - p. 53 - 53.
Making minor rural road networks safer: The effects of 60 km/h-zones
Jaarsma, C.F. ; Louwerse, W.J.R. ; Dijkstra, A. ; Vries, J.R. de; Spaas, J.P. - \ 2011
Accident Analysis and Prevention 43 (2011)4. - ISSN 0001-4575 - p. 1508 - 1515.
speed limits - accidents
For safety reasons a maximum speed limit of 60km/h has been applied to minor rural roads in the Netherlands since 1998. To support this structurally, a part of these roads have also received additional physical measures in a so-called "low cost design" that is expected to reduce the number of traffic casualties by 10-20%. This measure has been implemented as much as possible in an area oriented way. To measure the design's effectivity, road safety in 20 specific rural areas was studied for 5 years before changes were implemented and, on average, 3.5 years thereafter. The study examined 851km of roads, and a control study was done on 2105km of comparable roads with a speed limit of 80km/h. Both the study and the control roads are managed by water boards. Results show that the measures implemented on the roads in the 60km/h-zones had statistically significant effects (p
Zoonose Vibrio vulnificus: meldingsplicht raadzaam
Dijkstra, A. ; Haenen, O.L.M. ; Moller, L. - \ 2010
Nederlands Tijdschrift voor Geneeskunde 2010 (2010)154. - ISSN 0028-2162 - p. A2320 - A2320.
vibrio vulnificus - viskwekerijen - zoönosen - gezondheid - infectieziekten - vibrio vulnificus - fish farms - zoonoses - health - infectious diseases
Op de lijst van meldingsplichtige infectieziekten komen een aantal zoönosen voor, zoals pest, rabiës en leptospirose. De relatief onbekende zoönotische Vibrio vulnificus valt opmerkelijk genoeg niet onder de meldingsplichtige infectieziekten. Juist vanwege het zeer agressieve beloop van een invasieve V. vulnificus-infectie is een snelle diagnose van belang om adequaat medisch te kunnen handelen. Tevens lijkt V. vulnificus wereldwijd steeds vaker de kop op te steken, zelfs in landen waar men deze bacterie niet zou verwachten, zoals Spanje, Denemarken, Japan en ook Nederland.
Regulatory phenotyping reveals important diversity within the species Lactococcus lactis
Bachmann, H. ; Starrenburg, M. ; Dijkstra, A. ; Molenaar, D. ; Kleerebezem, M. ; Rademaker, J.L.W. ; Hylckama Vlieg, J.E.T. van - \ 2009
Applied and Environmental Microbiology 75 (2009)17. - ISSN 0099-2240 - p. 5687 - 5694.
escherichia-coli - flavor formation - streptococcus-cremoris - natural diversity - gene inactivation - parallel changes - acid bacteria - cheese - evolution - subsp
The diversity in regulatory phenotypes among a collection of 84 Lactococcus lactis strains isolated from dairy and nondairy origin was explored. The specific activities of five enzymes were assessed in cell extracts of all strains grown in two different media, a nutritionally rich broth and a relatively poor chemically defined medium. The five investigated enzymes, branched chain aminotransferase (BcaT), aminopeptidase N (PepN), X-prolyl dipeptidyl peptidase (PepX), alpha-hydroxyisocaproic acid dehydrogenase (HicDH), and esterase, are involved in nitrogen and fatty acid metabolism and catalyze key steps in the production of important dairy flavor compounds. The investigated cultures comprise 75 L. lactis subsp. lactis isolates (including 7 L. lactis subsp. lactis biovar diacetylactis isolates) and 9 L. lactis subsp. cremoris isolates. All L. lactis subsp. cremoris and 22 L. lactis subsp. lactis (including 6 L. lactis subsp. lactis biovar diacetylactis) cultures originated from a dairy environment. All other cultures originated from (fermented) plant materials and were isolated at different geographic locations. Correlation analysis of specific enzyme activities revealed significantly different regulatory phenotypes for dairy and nondairy isolates. The enzyme activities in the two investigated media were in general poorly correlated and revealed a high degree of regulatory diversity within this collection of closely related strains. To the best of our knowledge, these results represent the most extensive diversity analysis of regulatory phenotypes within a single bacterial species to date. The presented findings underline the importance of the availability of screening procedures for, e.g., industrially relevant enzyme activities in models closely mimicking application conditions. Moreover, they corroborate the notion that regulatory changes are important drivers of evolution
Fasciitis necroticans ten gevolge van een infectie met in een palingkwekerij
Dijkstra, A. ; Ingen, J. van; Lubbert, P.H.W. ; Haenen, O.L.M. ; Möller, A.V.M. - \ 2009
Nederlands Tijdschrift voor Geneeskunde 153 (2009)9. - ISSN 0028-2162 - p. 408 - 411.
vibrio vulnificus - palingteelt - palingen - viskwekerijen - visziekten - infecties - vibrio vulnificus - eel culture - eels - fish farms - fish diseases - infections
Een 41-jarige man presenteerde zich met een pijnlijke infectie van zijn rechter hand. Hij werkte in een palingkwekerij, waar op dat moment een infectie met Vibrio vulnificus onder de palingen heerste. Vibrio vulnificus als veroorzaker van een wondinfectie, een sepsis of een gastro-enteritis is een zeldzame ziekte, meestal een importziekte in ons land. In dit artikel bespreken de auteurs aan de hand van een casus de ernst van de aandoening, de wijze van besmetting en de zeldzaamheid van fasciitis necroticans veroorzaakt door V. vulnificus
Exploiting natural microbial biodiversity for development of flavour starters
Hylckama Vlieg, J.E.T. van; Dijkstra, A. ; Smit, B.A. ; Engels, W.J.M. ; Rijnen, L. ; Starrenburg, M.J.C. ; Smit, G. ; Wouters, J.A. - \ 2006
In: Flavour Science: recent advances and trends / Bredie, W.L.P., Petersen, M.A., Elsevier - ISBN 9780444527424 - p. 61 - 64.
Exploiting natural microbial diversity for development of flavour starters
Hylckama Vlieg, J.E.T. van; Dijkstra, A. ; Smit, B.A. ; Engels, W.J.M. ; Rijnen, L. ; Starrenburg, M.J.C. ; Smit, G. ; Wouters, J.A. - \ 2005
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