Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Isolation and characterization of H7N9 avian influenza A virus from humans with respiratory diseases in Zhejiang, China.
Zhang, Y. ; Mao, H. ; Yan, J. ; Zhang, L. ; Sun, Y. ; Wang, X. ; Chen, Y. ; Lu, Y. ; Chen, E. ; Lv, H. ; Gong, L. ; Li, Z. ; Gao, J. ; Xu, C. ; Feng, Y. ; Ge, Q. ; Xu, B. ; Xu, F. ; Yang, Z. ; Zhao, C. ; Han, J. ; Koch, G. ; Li, H. ; Shu, Y.L. ; Chen, Z. - \ 2014
Virus Research 189 (2014). - ISSN 0168-1702 - p. 158 - 164.
human infection - amino-acid - poultry - host - transmission - substitution - pathogenesis - determinant - adaptation - polymerase
In 2013, the novel reassortant avian-origin influenza A (H7N9) virus was reported in China. Through enhanced surveillance, infection by the H7N9 virus in humans was first identified in Zhejiang Province. Real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) was used to confirm the infection. Embryonated chicken eggs were used for virus isolation from pharyngeal swabs taken from infected human patients. The H7N9 isolates were first identified by the hemagglutination test and electron microscopy, then used for whole genome sequencing. Bioinformatics software was used to construct the phylogenetic tree and for computing the mean rate of evolution of the HA gene in H7Nx and NA in HxN9. Two novel H7N9 avian influenza A viruses (A/Zhejiang/1/2013 and A/Zhejiang/2/2013) were isolated from the positive infection cases. Substitutions were found in both Zhejiang isolates and were identified as human-type viruses. All phylogenetic results indicated that the novel reassortant in H7N9 originated in viruses that infected birds. The sequencing and phylogenetic analysis of the whole genome revealed the mean rate of evolution of the HA gene in H7NX to be 5.74E-3 (95% Highest posterior density: 3.8218E-3 to 7.7873E-3) while the NA gene showed 2.243E-3 (4.378E-4 to 3.79E-3) substitutions per nucleotide site per year. The novel reassortant H7N9 virus was confirmed by molecular methods to have originated in poultry, with the mutations occurring during the spread of the H7N9 virus infection. Live poultry markets played an important role in whole H7N9 circulation.
Complete genome sequence of Dehalobacter restrictus PER-K23T
Kruse, T.K. ; Maillard, J. ; Goodwin, L.A. ; Woyke, T. ; Teshima, H. ; Bruce, D.C. ; Detter, J.C. ; Tapia, R. ; Han, C. ; Huntemann, M. ; Wei, C.L. ; Han, J. ; Chen, A. ; Kyrpides, N. ; Szeto, E. ; Markowitz, V. ; Ivanova, N. ; Pagani, I. ; Pati, A. ; Pitluck, S. ; Nolan, M. ; Holliger, C. ; Smidt, H. - \ 2013
Standards in Genomic Sciences 8 (2013)3. - ISSN 1944-3277 - p. 375 - 388.
tetrachloroethene reductive dehalogenase - hafniense strain tce1 - desulfitobacterium-hafniense - dehalococcoides-ethenogenes - rna genes - bacteria - respiration - geobacter - protein - halorespiration
Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or trichloroethene. Growth has not been observed with any other electron donor or acceptor, nor has fermentative growth been shown. Here we introduce the first full genome of a pure culture within the genus Dehalobacter. The 2,943,336 bp long genome contains 2,826 protein coding and 82 RNA genes, including 5 16S rRNA genes. Interestingly, the genome contains 25 predicted reductive dehalogenase genes, the majority of which appear to be full length. The reductive dehalogenase genes are mainly located in two clusters, suggesting a much larger potential for organohalide respiration than previously anticipated
An overview of trade opportunities in China's pork chain
Enting, J. ; Han, J. ; Verstappen-Boerekamp, J.A.M. - \ 2009
Wageningen : Wageningen UR, Animal Sciences Group - 34
varkens - varkenshouderij - varkensvlees - vlees - pigs - pig farming - pigmeat - meat
Onderzoek naar de toekomstige mogelijkheden voor varkensvlees op de Chinese markt
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