DNA marking of some quantitative trait loci in the cultivated edible mushroom Pleurotus ostreatus (Fr.) Kumm
Sivolapova, A.B. ; Shnyreva, A.V. ; Sonnenberg, A.S.M. ; Baars, J.J.P. - \ 2012
Russian Journal of Genetics 48 (2012)4. - ISSN 1022-7954 - p. 383 - 389.
wheat-straw - effluent
Fungi of the genus Pleurotus, in particular, species Pleurotus ostreatus (common oyster mushroom) are among most cultivated fungi in the world. Due to intense rates of development of studies in this field, efficient breeding programs are highly required in the search for new P. ostreatus strains. The principal traits used worldwide for selecting strains are intensity of fruitbearing, fruit body cap color (for some consumptive markets), and mycelium growth rate. In this connection, the objective of this work was to study these quantitative traits and to find molecular markers, which could be employed to accomplish breeding programs. In general, we found 12 genomic loci (quantitative trait loci, QTLs) controlling mycelium growth rate of oyster and six QTLs responsible for the fruit body cap color. The genetic map of P. ostreatus was constructed, and all markers of quantitative traits found by us were located on this genetic map. The obtained linkage map can be a useful tool for the accomplishment of breeding programs to improve economically important traits of oyster mushroom.
Extracts of Medicinal Mushrooms Agaricus bisporus and Phellinus linteus Induce Proapoptotic Effects in the Human Leukemia Cell Line K562
Shnyreva, A.V. ; Song, W. ; Griensven, L.J.L.D. van - \ 2010
International Journal of Medicinal Mushrooms 12 (2010)2. - ISSN 1521-9437 - p. 167 - 175.
nf-kappa-b - interferon-gamma - apoptosis - mechanisms - expression - death - pcr
We have studied the effects of Agaricus bisporus and Phellinus linteus ethanol extracts on transcriptional regulation of genes involved in cytokine release and apoptosis in the human leukemia cell line K562. In particular, we applied quantitative real-time PCR (Q-PCR) assays to monitor alterations of gene expression for the following genes: Bcl-2, Casp-9, NF-¿B, TNF-a, IFN-¿, and IL-10. We found stronger proapoptotic activity for the Ph. linteus alcohol extract on K562 cells than for the A. bisporus extract: 4.4- and 2.2-fold increase of Bcl-2 and Casp-9 transcripts. Mushroom alcohol extracts are suggested to exert their effects on tumor cells via the induction of apoptosis. K562 leukemia cells were shown to be most responsive to the transcriptional induction of tumor necrosis factor TNF-a when stimulated with IFN-¿ and then treated with Ph. linteus alcohol extract: up to a 4.5-fold increase. Treatment of K562 cells with A. bisporus extract promoted transcription of the cytokine gene IL-10