Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Reply to Kascsak: Definition of the PrP 3F4 Epitope Revisited
Zou, W.Q. ; Langeveld, J.P.M. ; Xiao, X.Z. ; Chen, S.G. ; McGeer, P.L. ; Yuan, J. ; Payne, M.C. ; Kang, H.E. ; McGeehan, J. ; Sy, M.S. ; Greenspan, N.S. ; Kaplan, D. ; Wang, G.X. ; Parchi, P. ; Hoover, E. ; Kneale, G. ; Telling, G. ; Surewicz, W.K. ; Kong, Q.Z. ; Guo, J.P. - \ 2010
Journal of Biological Chemistry 285 (2010)20. - ISSN 0021-9258 - p. le6 - le6.
protein - antibody
PrP Conformational Transitions Alter Species Preference of a PrP-specific antibody
Zou, W.Q. ; Langeveld, J.P.M. ; Xiao, X. ; Chen, S. ; McGeer, P.L. ; Yuan, J. ; Payne, M.C. ; Kang, H.E. ; McGeehan, J.M. ; Sy, M.S. ; Greenspan, N.S. ; Kaplan, D. ; Wang, G.X. ; Parchi, P. ; Hoover, E.A. ; Kneale, G. ; Telling, G. ; Surewicz, W. ; Kong, Q. ; Guo, J. - \ 2010
Journal of Biological Chemistry 285 (2010). - ISSN 0021-9258 - p. 13874 - 13884.
creutzfeldt-jakob-disease - pathological prion protein - monoclonal-antibody - n-terminus - scrapie - epitope - binding - brain - recognition - strain
The epitope of the 3F4 antibody most commonly used in human prion disease diagnosis is believed to consist of residues Met-Lys-His-Met (MKHM) corresponding to human PrP-(109–112). This assumption is based mainly on the observation that 3F4 reacts with human and hamster PrP but not with PrP from mouse, sheep, and cervids, in which Met at residue 112 is replaced by Val. Here we report that, by brain histoblotting, 3F4 did not react with PrP of uninfected transgenic mice expressing elk PrP; however, it did show distinct immunoreactivity in transgenic mice infected with chronic wasting disease. Compared with human PrP, the 3F4 reactivity with the recombinant elk PrP was 2 orders of magnitude weaker, as indicated by both Western blotting and surface plasmon resonance. To investigate the molecular basis of these species- and conformer-dependent preferences of 3F4, the epitope was probed by peptide membrane array and antigen competition experiments. Remarkably, the 3F4 antibody did not react with MKHM but reacted strongly with KTNMK (corresponding to human PrP-(106–110)), a sequence that is also present in cervids, sheep, and cattle. 3F4 also reacted with elk PrP peptides containing KTNMKHV. We concluded that the minimal sequence for the 3F4 epitope consists of residues KTNMK, and the species- and conformer-dependent preferences of 3F4 arise largely from the interactions between Met112 (human PrP) or Val115 (cervid PrP) and adjacent residues.
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