- K. Bourtzis (1)
- I.A. Ince (2)
- H.M. Kariithi (1)
- H.M. Karrithi (1)
- N.K. Maniania (1)
- M.M. Oers van (2)
- A.G. Parker (2)
- J.M. Vlak (3)
Improving Sterile Insect Technique (SIT) for tsetse flies through research on their symbiont and pathogens
Abd-Alla, A.M.M. ; Bergoin, M. ; Parker, A.G. ; Maniania, N.K. ; Vlak, J.M. ; Bourtzis, K. ; Boucias, D.G. ; Aksoy, S. - \ 2013
Journal of Invertebrate Pathology 112 (2013)suppl. 1. - ISSN 0022-2011 - p. S2 - S10.
salivary-gland hypertrophy - glossina-palpalis-gambiensis - rickettsia-like-organisms - sleeping sickness foci - wigglesworthia-glossinidia - sodalis-glossinidius - musca-domestica - wolbachia infections - genetic diversity - house-flies
Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of the trypanosomes, which cause human African trypanosomosis (HAT) or sleeping sickness in humans and African animal trypanosomosis (AAT) or nagana in animals. Due to the lack of effective vaccines and inexpensive drugs for HAT, and the development of resistance of the trypanosomes against the available trypanocidal drugs, vector control remains the most efficient strategy for sustainable management of these diseases. Among the control methods used for tsetse flies, Sterile Insect Technique (SIT), in the frame of area-wide integrated pest management (AW-IPM), represents an effective tactic to suppress and/or eradicate tsetse flies. One constraint in implementing SIT is the mass production of target species. Tsetse flies harbor obligate bacterial symbionts and salivary gland hypertrophy virus which modulate the fecundity of the infected flies. In support of the future expansion of the SIT for tsetse fly control, the Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture implemented a six year Coordinated Research Project (CRP) entitled “Improving SIT for Tsetse Flies through Research on their Symbionts and Pathogens”. The consortium focused on the prevalence and the interaction between the bacterial symbionts and the virus, the development of strategies to manage virus infections in tsetse colonies, the use of entomopathogenic fungi to control tsetse flies in combination with SIT, and the development of symbiont-based strategies to control tsetse flies and trypanosomosis. The results of the CRP and the solutions envisaged to alleviate the constraints of the mass rearing of tsetse flies for SIT are presented in this special issue.
The salivary secretome of the tsetse fly Glossina pallipides (Diptera: Glossinidae) infected by salivary gland hypertrophy virus
Kariithi, H.M. ; Ince, I.A. ; Boeren, S. ; Abd-Alla, A.M.M. ; Parker, A.G. ; Aksoy, S. ; Vlak, J.M. ; Oers, M.M. van - \ 2011
PLoS Neglected Tropical Diseases 5 (2011)11. - ISSN 1935-2727 - 14 p.
occlusion-derived virus - californica multiple nucleopolyhedrovirus - envelope protein p74 - transcription factor tfiib - synthase gene-expression - os infectivity factor - actin-based motility - human hsp70 promoter - heat-shock proteins - c-type lectin
Background The competence of the tsetse fly Glossina pallidipes (Diptera; Glossinidae) to acquire salivary gland hypertrophy virus (SGHV), to support virus replication and successfully transmit the virus depends on complex interactions between Glossina and SGHV macromolecules. Critical requisites to SGHV transmission are its replication and secretion of mature virions into the fly's salivary gland (SG) lumen. However, secretion of host proteins is of equal importance for successful transmission and requires cataloging of G. pallidipes secretome proteins from hypertrophied and non-hypertrophied SGs. Methodology/Principal Findings After electrophoretic profiling and in-gel trypsin digestion, saliva proteins were analyzed by nano-LC-MS/MS. MaxQuant/Andromeda search of the MS data against the non-redundant (nr) GenBank database and a G. morsitans morsitans SG EST database, yielded a total of 521 hits, 31 of which were SGHV-encoded. On a false discovery rate limit of 1% and detection threshold of least 2 unique peptides per protein, the analysis resulted in 292 Glossina and 25 SGHV MS-supported proteins. When annotated by the Blast2GO suite, at least one gene ontology (GO) term could be assigned to 89.9% (285/317) of the detected proteins. Five (~1.8%) Glossina and three (~12%) SGHV proteins remained without a predicted function after blast searches against the nr database. Sixty-five of the 292 detected Glossina proteins contained an N-terminal signal/secretion peptide sequence. Eight of the SGHV proteins were predicted to be non-structural (NS), and fourteen are known structural (VP) proteins. Conclusions/Significance SGHV alters the protein expression pattern in Glossina. The G. pallidipes SG secretome encompasses a spectrum of proteins that may be required during the SGHV infection cycle. These detected proteins have putative interactions with at least 21 of the 25 SGHV-encoded proteins. Our findings opens venues for developing novel SGHV mitigation strategies to block SGHV infections in tsetse production facilities such as using SGHV-specific antibodies and phage display-selected gut epithelia-binding peptides
The salivary secretome of salivary gland hypertrophy virus-infected tsetse fly Glossina pallipides (Diptera: Glossinidae)
Karrithi, H.M. ; Ince, I.A. ; Boeren, S. ; Abd-Alla, A.M.M. ; Oers, M.M. van; Aksoy, S. ; Vlak, J.M. - \ 2011
In: Abstract Book of the 44th Annual Meeting of the Society for Invertebrate Pathology, Halifax, Nova Scotia, Canada, 7 - 11 August, 2011. - Halifax : - p. 45 - 45.