New records and updated checklist of mosquitoes (Diptera: Culicidae) from Lao People's Democratic Republic, with special emphasis on adult and larval surveillance in Khammuane Province
Motoki, Maysa T. ; Vongphayloth, Khamsing ; Rueda, Leopoldo M. ; Miot, Elliott F. ; Hiscox, Alexandra ; Hertz, Jeffrey C. ; Brey, Paul T. - \ 2019
Journal of Vector Ecology 44 (2019)1. - ISSN 1081-1710 - p. 76 - 88.
Culicidae - DNA barcode - Khammuane Province - Lao PDR - mosquito species list - mosquito surveillance
A list of mosquitoes from the Nakai Nam Theun National Protected Area along the Nam Theun, Nam Mon, Nam Noy, and Nam On rivers, Nakai District, Khammuane Province, Lao People's Democratic Republic (Lao PDR) is presented. Fifty-four mosquito taxa were identified, including 15 new records in the Lao PDR. A fragment of the mtDNA cytochrome c oxidase subunit I (COI) gene, barcode region, was generated for 34 specimens, and together with four specimens already published, it represented 23 species in eight genera. In addition, an updated checklist of 170 mosquito taxa from Lao PDR is provided based on field collections from Khammuane Province, the literature, and specimens deposited in the Smithsonian Institution, National Museum of Natural History (SI-NMNH), Washington, DC, U.S.A. This paper provides additional information about the biodiversity of mosquito fauna in Lao PDR.
Armigeres subalbatus colonization of damaged pit latrines : A nuisance and potential health risk to residents of resettlement villages in Laos
Hiscox, A. ; Hirooka, R. ; Vongphayloth, K. ; Hill, N. ; Lindsay, S.W. ; Grandadam, M. ; Brey, P.T. - \ 2016
Medical and Veterinary Entomology 30 (2016)1. - ISSN 0269-283X - p. 95 - 100.
Armigeres - Flavivirus - Laos - Latrine - Nuisance biting - 016-3917
During the resettlement of 6500 persons living around the Nam Theun 2 hydroelectric project in Laos, more than 1200 pour-flush latrines were constructed. To assess the role of these latrines as productive larval habitats for mosquitoes, entomological investigations using Centers for Disease Control (CDC) light traps, visual inspection and emergence trapping were carried out in over 300 latrines during the rainy seasons of 2008-2010. Armigeres subalbatus (Diptera: Culicidae) were nine times more likely to be found in latrines (mean catch: 3.09) than in adjacent bedrooms (mean catch: 0.37) [odds ratio (OR) 9.08, 95% confidence interval (CI) 6.74-15.11] and mosquitoes were active in and around 59% of latrines at dusk. Armigeres subalbatus was strongly associated with latrines with damaged or improperly sealed septic tank covers (OR 5.44, 95% CI 2.02-14.67; P <0.001). Armigeres subalbatus is a nuisance biter and a putative vector of Japanese encephalitis and dengue viruses. Dengue virus serotype 3 was identified from a single pool of non-blood-fed female A. subalbatus using reverse transcription polymerase chain reaction. Maintaining a good seal around septic tanks by covering them with a layer of soil is a simple intervention to block mosquito exit/entry and contribute to vector control in resettlement villages. The scale-up of this simple, cheap intervention would have global impact in preventing the colonization of septic tanks by nuisance biting and disease-transmitting mosquitoes.
Risk factors for the presence of Aedes aegypti and Aedes albopictus in domestic water-holding containers in areas impacted by the Nam Theun 2 hydroelectric project, Laos
Hiscox, A.F. ; Kaye, A. ; Vongphayloth, K. ; Banks, I. ; Piffer, M. ; Khammanithong, P. ; Sananikhom, P. ; Kaul, S. ; Hill, N. ; Lindsay, S.W. ; Brey, P.T. - \ 2013
American Journal of Tropical Medicine and Hygiene 88 (2013)6. - ISSN 0002-9637 - p. 1070 - 1078.
dengue vector control - diptera-culicidae - singapore-city - mosquitos - l. - insecticide - thailand - vietnam - skuse - surveillance
We assessed risk factors for vectors of dengue and chikungunya viruses near a new hydroelectric project, Nam Theun 2, in Laos. Immature stages of Aedes aegypti were found only in sites within 40 km of the urban provincial capital, but Aedes albopictus was found throughout. Aedes aegypti pupae were most common in water storage jars (odds ratio [OR] = 4.72) and tires (OR = 2.99), and Ae. albopictus pupae were associated with tires in 2009 (OR = 10.87) and drums, tires, and jars in 2010 (drums OR = 3.05; tires OR = 3.45, jars OR = 6.59). Compared with water storage vessels, containers used for hygiene, cooking, and drinking were 80% less likely to harbor Ae. albopictus pupae in 2010 (OR = 0.20), and discarded waste was associated with a 3.64 increased odds of infestation. Vector control efforts should focus on source reduction of water storage containers, particularly concrete jars and tires.