Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

    • help
    • print

      Print search results

    • export

      Export search results

    • alert
      We will mail you new results for this query: metisnummer==1152960
    Check title to add to marked list
    Crystal structure of the CRISPR RNA–guided surveillance complex from Escherichia coli
    Jackson, R.N. ; Golden, S.M. ; Erp, P.B. ; Carter, J. ; Westra, E.R. ; Brouns, S.J.J. ; Oost, J. van der; Terwilliger, T.C. ; Read, R.J. ; Wiedenheft, B. - \ 2014
    Science 345 (2014)6203. - ISSN 0036-8075 - p. 1473 - 1479.
    bacterial immune-system - processes pre-crrna - thermus-thermophilus - cas systems - interference complex - target recognition - antiviral defense - seed sequence - dna - cascade
    Clustered regularly interspaced short palindromic repeats (CRISPRs) are essential components of RNA-guided adaptive immune systems that protect bacteria and archaea from viruses and plasmids. In Escherichia coli, short CRISPR-derived RNAs (crRNAs) assemble into a 405-kilodalton multisubunit surveillance complex called Cascade (CRISPR-associated complex for antiviral defense). Here we present the 3.24 angstrom resolution x-ray crystal structure of Cascade. Eleven proteins and a 61-nucleotide crRNA assemble into a seahorse-shaped architecture that binds double-stranded DNA targets complementary to the crRNA-guide sequence. Conserved sequences on the 3' and 5' ends of the crRNA are anchored by proteins at opposite ends of the complex, whereas the guide sequence is displayed along a helical assembly of six interwoven subunits that present five-nucleotide segments of the crRNA in pseudo–A-form configuration. The structure of Cascade suggests a mechanism for assembly and provides insights into the mechanisms of target recognition.
    Check title to add to marked list

    Show 20 50 100 records per page

    Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.