- D.C. António (1)
- Simone Belluco (1)
- Greet Bemmel van (1)
- Giancarlo Biancotto (1)
- Olga Borovinskaya (1)
- Felice C. Simeone (1)
- Peter C. Tromp (1)
- Davide Calestani (1)
- L. Calzolai (1)
- Claudia Cascio (3)
- C. Cascio (1)
- Salvatore Catania (1)
- Veronica Cibin (1)
- Federica Gallocchio (1)
- Federica Gobbo (1)
- Ruud J.B. Peters (1)
- Hans J.P. Marvin (1)
- Z. Jakšić (1)
- D. Jurašin (1)
- Anna K. Undas (1)
- Carmen Losasso (1)
- D.M. Lyons (1)
- Monica Mattarozzi (1)
- Steffi Naasz (1)
- A.J.A. Nogueira (1)
- Ruud Peters (2)
- Antonia Ricci (1)
- F. Rossi (1)
- Andrius Serva (1)
- Michele Suman (1)
- Anna Undas (1)
- Stefan Weigel (3)
Multi-element analysis of single nanoparticles by ICP-MS using quadrupole and time-of-flight technologies
Naasz, Steffi ; Weigel, Stefan ; Borovinskaya, Olga ; Serva, Andrius ; Cascio, Claudia ; Undas, Anna K. ; Simeone, Felice C. ; Marvin, Hans J.P. ; Peters, Ruud J.B. - \ 2018
Journal of Analytical Atomic Spectrometry 33 (2018)5. - ISSN 0267-9477 - p. 835 - 845.
Determining composition, shape, and size of nanoparticles dispersed in a complex matrix is necessary in the assessment of toxicity, for regulatory actions, and environmental monitoring. Many types of nanoparticles that are currently used in consumer products contain more than one metal which are often not uniformly distributed (e.g., core-shell nanoparticles). This compositional and structural complexity makes their characterization difficult. In this study, we investigate the capability of single particle inductively coupled plasma mass spectrometry (spICP-MS) using time-of-flight (TOF) and quadrupole (Q) mass analyzers to determine the composition, size distribution, and concentration of a series of nanoparticles that are used in a variety of industrial applications: BiVO4, (Bi0.5Na0.5)TiO3 and steel (which contains Fe, Cr, Ni, Mo) nanoparticles. In addition, we tested both types of mass analyzers with Au-core/Ag-shell nanoparticles, which are well-characterized and have already been used for assessment of multi-element capabilities of spICP-MS. The results confirm that both types of mass analyzers produce accurate estimations of the size of Au-core/Ag-shell particles. For other multi-element nanoparticles, spICP-MS provided the size of aggregates and/or agglomerates in the prepared suspensions. In general, particle size detection limits (dLOD) of spICP-TOFMS instruments with values of 29 nm for Ti, 14 nm for Mo, and 7 nm for Au, are smaller than those obtained for the quadrupole instruments. This study finds that only spICP-TOFMS can accurately assess the elemental composition of nano-steel particles. By contrast, spICP-QMS is limited to the detection of 2 elements in an individual particle and the elemental composition of nano-steel particles is less accurate. In general, spICP-TOFMS was able to quantify multiple elements with high precision and that currently makes it the first choice for multi-element detection of unknown nanoparticles.
Transfer Study of Silver Nanoparticles in Poultry Production
Gallocchio, Federica ; Biancotto, Giancarlo ; Cibin, Veronica ; Losasso, Carmen ; Belluco, Simone ; Peters, Ruud ; Bemmel, Greet van; Cascio, Claudia ; Weigel, Stefan ; Tromp, Peter C. ; Gobbo, Federica ; Catania, Salvatore ; Ricci, Antonia - \ 2017
Journal of Agricultural and Food Chemistry 65 (2017)18. - ISSN 0021-8561 - p. 3767 - 3774.
chicken - nanomaterials - silver - silver nanoparticles - spICP-MS - transfer
Silver nanoparticles (AgNPs) are of interest due to their antimicrobial activity and are seen as potential candidates to replace antibiotics in animal husbandry. A few studies have focused on this new application, but they lack any considerations about residual accumulation of AgNPs in edible animal tissues and animal products. In this research, a 22 day in vivo study was carried out by oral administration of 20 nm spherical PVP coated AgNPs to hens. Six doses of approximately 1 mg kg-1 of AgNPs-PVP each were administered to animals throughout the experimentation. Atomic absorption spectroscopy (AAS) was used for quantitative determination of residual total Ag in different organs and matrices. The analyses showed that Ag accumulates in livers (concentration ranging from 141 μg kg-1 to 269 μg kg-1) and yolks (concentration ranging from 20 μg kg-1 to 49 μg kg-1) but not in muscles, kidneys, and albumen belonging to hens of the treated group (tG2). Ag was not detected in animals of the control group (uG1) (i.e., total Ag < LOD = 10 μg kg-1). Single particle inductively coupled plasma mass spectrometry (spICP-MS) and scanning electron microscopy with energy dispersive X-ray detection (SEM-EDX) were employed to elucidate the presence of AgNPs in livers and yolks belonging to tG2 animals. spICP-MS highlighted that part of residual Ag found in livers (about 5-20%) is in NP form with an average dimension of approximately 20 nm. SEM-EDX technique confirmed the presence of AgNPs only in livers of treated animals. The results show that feeding AgNPs to hens may become a source of consumer exposure to AgNPs. As far as we know this is the first study showing transfer of AgNPs or reaction products thereof from animal feed to animal products.
Analytical approaches for the characterization and quantification of nanoparticles in food and beverages
Mattarozzi, Monica ; Suman, Michele ; Cascio, Claudia ; Calestani, Davide ; Weigel, Stefan ; Undas, Anna ; Peters, Ruud - \ 2017
Analytical and Bioanalytical Chemistry 409 (2017). - ISSN 1618-2642 - p. 63 - 80.
Analytical methods - Emerging contaminants - Food - Nanomaterials - Nanoparticles - Risk assessment
Estimating consumer exposure to nanomaterials (NMs) in food products and predicting their toxicological properties are necessary steps in the assessment of the risks of this technology. To this end, analytical methods have to be available to detect, characterize and quantify NMs in food and materials related to food, e.g. food packaging and biological samples following metabolization of food. The challenge for the analytical sciences is that the characterization of NMs requires chemical as well as physical information. This article offers a comprehensive analysis of methods available for the detection and characterization of NMs in food and related products. Special attention was paid to the crucial role of sample preparation methods since these have been partially neglected in the scientific literature so far. The currently available instrumental methods are grouped as fractionation, counting and ensemble methods, and their advantages and limitations are discussed. We conclude that much progress has been made over the last 5 years but that many challenges still exist. Future perspectives and priority research needs are pointed out. [Figure not available: see fulltext.]
Assessing silver nanoparticles behaviour in artificial seawater by mean of AF4 and spICP-MS
António, D.C. ; Cascio, C. ; Jakšić, Z. ; Jurašin, D. ; Lyons, D.M. ; Nogueira, A.J.A. ; Rossi, F. ; Calzolai, L. - \ 2015
Marine Environmental Research 111 (2015). - ISSN 0141-1136 - p. 162 - 169.
Alginate - Asymmetric flow field-flow fractionation - Humic acid - Metal-organic interactions - Salinity - Silver nanoparticles - Single particle ICP-MS
The use of nanotechnology-based products is constantly increasing and there are concerns about the fate and effect on the aquatic environment of antimicrobial products such as silver nanoparticles. By combining different characterization techniques (asymmetric flow field-flow fractionation, single particle ICP-MS, UV-Vis) we show that it is possible to assess in detail the agglomeration process of silver nanoparticles in artificial seawater. In particular we show that the presence of alginate or humic acid differentially affects the kinetic of the agglomeration process. This study provides an experimental methodology for the in-depth analysis of the fate and behaviour of silver nanoparticles in the aquatic environment.