Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Training of primary chicken monocytes results in enhanced pro-inflammatory responses
    Verwoolde, Michel B. ; Biggelaar, Robin H.G.A. van den; Baal, Jürgen van; Jansen, Christine A. ; Lammers, Aart - \ 2020
    Veterinary Sciences 7 (2020)3. - ISSN 2306-7381
    Flow cytometry - Inflammatory response - Innate immune memory - Macrophages - Primary chicken monocytes - Β-glucan

    Beta-glucan-stimulated mammalian myeloid cells, such as macrophages, show an increased responsiveness to secondary stimulation in a nonspecific manner. This phenomenon is known as trained innate immunity and is important to prevent reinfections. Trained innate immunity seems to be an evolutionary conserved phenomenon among plants, invertebrates and mammalian species. Our study aimed to explore the training of primary chicken monocytes. We hypothesized that primary chicken monocytes, similar to their mammalian counterparts, can be trained with β-glucan resulting in increased responses of these cells to a secondary stimulus. Primary blood monocytes of white leghorn chickens were primary stimulated with β-glucan microparticulates (M-βG), lipopolysaccharide (LPS), recombinant chicken interleukin-4 (IL-4) or combinations of these components for 48 h. On day 6, the primary stimulated cells were secondary stimulated with LPS. Nitric oxide (NO) production levels were measured as an indicator of pro-inflammatory activity. In addition, the cells were analyzed by flow cytometry to characterize the population of trained cells and to investigate the expression of surface markers associated with activation. After the secondary LPS stimulation, surface expression of colony stimulating factor 1 receptor (CSF1R) and the activation markers CD40 and major histocompatibility complex class II (MHC-II) was higher on macrophages that were trained with a combination of M-βG and IL-4 compared to unstimulated cells. This increased expression was paralleled by enhanced NO production. In conclusion, this study showed that trained innate immunity can be induced in primary chicken monocytes with β-glucan, which is in line with previous experiments in mammalian species. Innate immune training may have the potential to improve health and vaccination strategies within the poultry sector.

    Innate immune training and metabolic reprogramming in primary monocytes of broiler and laying hens
    Verwoolde, Michel B. ; Biggelaar, Robin H.G.A. van den; Vries Reilingh, Ger de; Arts, Joop A.J. ; Baal, Jürgen van; Lammers, Aart ; Jansen, Christine A. - \ 2020
    Developmental and Comparative Immunology 114 (2020). - ISSN 0145-305X
    Chickens - Immune training - Lipopolysaccharide - Metabolism - Monocytes - β-glucan

    Recently, we have reported trained innate immunity in laying chicken monocytes. In the present study, we further investigated trained innate immunity of monocytes in layers and broilers. Monocytes of both breeds isolated from blood were trained in vitro with β-glucan, rec-chicken IL-4 or a combination of both, and restimulated with lipopolysaccharide (LPS), after which inflammation and metabolism-related responses were measured. Training of laying and broiler hen monocytes resulted in increased mRNA levels of IL-1β, iNOS and HIF-1α, but enhanced surface expression of CD40 and NO production was only observed in layers. Our in vitro study demonstrates that monocytes from different genetic backgrounds can be trained. However, the observed differences suggest a differential effect on immune functionality associated with innate training. Whether these differences in immune functions between layers and broilers have effect on disease resistance remains to be elucidated.

    The influence of genetic background on trained innate immunity in chicken macrophages
    Verwoolde, M.B. ; Biggelaar, Robin H.G.A. van den; Baal, J. van; Jansen, Christine A. ; Lammers, A. - \ 2019
    Chicken lines divergently selected on feather pecking differ in immune characteristics
    Eijk, Jerine A.J. van der; Verwoolde, Michel B. ; Vries Reilingh, Ger de; Jansen, Christine A. ; Rodenburg, Bas ; Lammers, Aart - \ 2019
    Physiology and Behavior 212 (2019). - ISSN 0031-9384
    Feather pecking - Immune system - Natural (auto)antibodies - Nitric oxide production - Specific antibodies

    It is crucial to identify whether relations between immune characteristics and damaging behaviors in production animals exist, as these behaviors reduce animal welfare and productivity. Feather pecking (FP) is a damaging behavior in chickens, which involves hens pecking and pulling at feathers of conspecifics. To further identify relationships between the immune system and FP we characterized high FP (HFP) and low FP (LFP) selection lines with regard to nitric oxide (NO) production by monocytes, specific antibody (SpAb) titers, natural (auto)antibody (N(A)Ab) titers and immune cell subsets. NO production by monocytes was measured as indicator for innate pro-inflammatory immune functioning, SpAb titers were measured as part of the adaptive immune system and N(A)Ab titers were measured as they play an essential role in both innate and adaptive immunity. Immune cell subsets were measured to identify whether differences in immune characteristics were reflected by differences in the relative abundance of immune cell subsets. Divergent selection on FP affected NO production by monocytes, SpAb and N(A)Ab titers, but did not affect immune cell subsets. The HFP line showed higher NO production by monocytes and higher IgG N(A)Ab titers compared to the LFP line. Furthermore the HFP line tended to have lower IgM NAAb titers, but higher IgM and IgG SpAb titers compared to the LFP line. Thus, divergent selection on FP affects the innate and adaptive immune system, where the HFP line seems to have a more responsive immune system compared to the LFP line. Although causation cannot be established in the present study, it is clear that relationships between the immune system and FP exist. Therefore, it is important to take these relationships into account when selecting on behavioral or immunological traits.

    Trained innate immunity in chicken macrophages
    Verwoolde, Michel - \ 2019
    Trained innate immunity in chicken macrophages
    Verwoolde, M.B. ; Biggelaar, Robin H.G.A. van den; Baal, J. van; Jansen, Christine A. ; Lammers, A. - \ 2019
    In: Trade-offs in science – keeping the Balance. - Wageningen University & Research - p. 27 - 27.
    In vitro model to study trained innate immunity in chicken primary monocytes
    Verwoolde, M.B. ; Biggelaar, Robin H.G.A. van den; Baal, J. van; Jansen, Christine A. ; Lammers, A. - \ 2018
    Long-term effects of early life microbiota disturbance on adaptive immunity in laying hens
    Simon, K. ; Verwoolde, M.B. ; Zhang, J. ; Smidt, H. ; Vries Reilingh, G. De; Kemp, B. ; Lammers, A. - \ 2016
    Poultry Science 95 (2016)7. - ISSN 0032-5791 - p. 1543 - 1554.
    Antibiotics - Chicken - Immune response - Microbiota

    Due to an interplay between intestinal microbiota and immune system, disruption of intestinal microbiota composition during immune development may have consequences for immune responses later in life. The present study investigated the effects of antibiotic treatment in the first weeks of life on the specific antibody response later in life in chickens. Layer chicks received an antibiotic cocktail consisting of vancomycin, neomycin, metronidazole, and amphotericin-B by oral gavage every 12 h, and ampicillin and colistin in drinking water for the first week of life. After the first week of life, chicks received ampicillin and colistin in drinking water for two more weeks. Control birds received no antibiotic cocktail and plain drinking water. Fecal microbiota composition was determined during antibiotic treatment (d 8 and 22), two weeks after cessation of antibiotic treatment (d 36), and at the end of the experimental period at d 175 using a 16S ribosomal RNA gene targeted microarray, the Chicken Intestinal Tract Chip (ChickChip). During antibiotic treatment fecal microbiota composition differed strongly between treatment groups. Fecal microbiota of antibiotic treated birds consisted mainly of Proteobacteria, and in particular E.coli, whereas fecal microbiota of control birds consisted mainly of Firmicutes, such as lactobacilli and clostridia. Two weeks after cessation of antibiotic treatment fecal microbiota composition of antibiotic treated birds had recovered and was similar to that of control birds. On d 105, 12 weeks after cessation of antibiotic treatment, chicks of both treatment groups received an intra-tracheal lipopolysaccharide (LPS)/human serum albumin (HuSA) challenge. Antibody titers against LPS and HuSA were measured 10 days after administration of the challenge. While T cell independent antibody titers (LPS) were not affected by antibiotic treatment, antibiotic treated birds showed lower T cell dependent antibody titers (HuSA) compared with control birds. In conclusion, intestinal microbial dysbiosis early in life may still have effects on the specific antibody response months after cessation of antibiotic treatment and despite an apparent recovery in microbiota composition.

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