Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Evaluation of an in vitro fibre fermentation method using feline faecal inocula : inter-individual variation
Bosch, Guido ; Heesen, Lisa ; Melo Santos, Karine de; Cone, John W. ; Pellikaan, Wilbert F. ; Hendriks, Wouter H. - \ 2017
Journal of Nutritional Science 6 (2017). - ISSN 2048-6790 - 4 p.
Cats - Fibre fermentability - Gas production - In vitro methodology - SCFA

The present study aimed to evaluate the inter-individual variability in fermentation of standard fibrous substrates by faecal inocula from ten healthy adult female cats. Substrates were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from each cat. Gas production was measured continuously during the 48 h incubation and SCFA and organic matter disappearance (only SBP and WM) after incubation. Out of ten cats, nine produced faeces on the days of inoculum preparation. The substrates contrasted in terms of fermentation parameters measured. The inter-individual variability was in general lower for the more simple and pure substrates (CP, FOS, GG) than for the more complex substrates containing mixtures of fibres (SBP, WM). Furthermore, for total SCFA and gas produced, inter-individual variability was lower than for proportions of butyrate and of branched-chain fatty acids and for the parameters of gas production kinetics. It is concluded that the variability in in vitro fermentation parameters is associated with the complexity of fibrous substrates. The presented data are instrumental for the calculation of number of faecal donors required for precise in vitro characterisation of the fermentability of dietary fibres. In addition, the number of faecal donors should be adjusted to the specific fermentation parameter(s) of interest.

Evaluation of an in vitro fibre fermentation method using feline faecal inocula : repeatability and reproducibility
Bosch, Guido ; Heesen, Lisa ; Melo Santos, Karine de; Pellikaan, Wilbert F. ; Cone, John W. ; Hendriks, Wouter H. - \ 2017
Journal of Nutritional Science 6 (2017). - ISSN 2048-6790 - 4 p.
Cats - Fibre fermentability - Gas production - In vitro methodology - SCFA

To gain knowledge on the precision of an in vitro method for characterisation of the fermentability of dietary fibres, this study aimed to evaluate the repeatability and reproducibility of such a method. Substrates used were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from five cats with three replicates for each substrate–cat combination. Gas production was measured continuously during the 48 h incubation and SCFA and organic matter disappearance (only SBP and WM) were determined after incubation. Four consecutive runs were performed. The within-run variability (repeatability) was generally lower for the more simple and pure substrates (CP, FOS, GG) than for the more complex substrates containing mixtures of fibres (SBP, WM). Replicates showed high variability, in particular for SCFA profiles and parameters of gas production kinetics. The between-run CV (reproducibility) for the measured parameters were, in general, below 10 % for CP, FOS and GG and higher values were obtained for SBP and WM. It is concluded that for precise dietary fibre characterisation, the number of replicates should be multiple and adjusted according to the variability of the parameters of interest and the complexity of fibres. The method yielded reproducible results with some variation in absolute values obtained, which may have an impact on the significance level of the differences among substrates.

Evaluation of an in vitro fibre fermentation method using feline faecal inocula: inter-individual variation
Bosch, G. ; Heesen, L. ; Melo Santos, Karine de; Cone, J.W. ; Pellikaan, W.F. ; Hendriks, W.H. - \ 2016
In: Growing science in pet nutrition. - Waltham Centre for Pet Nutrition - p. 135 - 136.
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