Characterisation of fish leucocytes : an immunocytochemical and functional study in carp (Cyprinus carpio L.)
Koumans - van Diepen, J.C.E. - \ 1993
Agricultural University. Promotor(en): W.B. van Muiswinkel, co-promotor(en): J.H.W.M. Rombout. - S.l. : Koumans-van Diepen - ISBN 9789054851110 - 167
cyprinidae - karper - bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - reticulo-endotheliaal systeem - antilichamen - immunoglobulinen - immunocytochemie - cyprinidae - carp - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma - reticuloendothelial system - antibodies - immunoglobulins - immunocytochemistry
A panel of monoclonal antibodies (MAbs) against carp serum immunoglobulin (Ig), WCIs or carp thymocytes (T), WCTs were used for the characterisation of carp leucocytes. Unfortunately, all WCTs and some WCIs react with common carbohydrate determinants present on all leucocytes and Ig. Most WCIs react specific with protein determinants at the heavy chain of Ig. Consequently, B lymphocyte (sub) populations, plasma cells and Ig-binding cells could be studied. Ig molecules are found in clusters at the cell membrane of B cells and plasma cells, and in contrast to mammalian plasma cells, most carp plasma cells still have Ig at their surface membrane. Mainly the dull surface Ig-positive (sIg +) cells were stimulated by the mammalian B cell mitogen LPS and not by PHA (T cell mitogen) in vitro , whereas the sIg-negative (sIg -) cells were stimulated by PHA and not by LPS. The percentages of B cells and plasma cells showed an increase during ontogeny and reached a plateau at about 3 months and 8 months of age respectively. It is suggested that full development of the carp (humoral) immune system needs at least 8 months (at 21-22 °C). Three different subpopulations of B cells and plasma cells and at least two Ig isotypes can be distinguished based upon their reactivity with WCI 4 arid WCI 12. The distribution of the three B cell subpopulations appeared to be organ and age dependent which indicates functional differences between the Ig isotypes. Fc-like receptors were mainly demonstrated on gut macrophages while pronephros macrophages and neutrophilic granulocytes did not show Ig binding. Consequently, other forms of antigen opsonisation (e.g. complement) may play a role in phagocytosis by these non Ig-binding cells. Several procedures were tested for obtaining MAbs specific for Ig -lymphoid cells. It is concluded that the presence of immunodominant carbohydrate determinants is the major problem for obtaining specific MAbs. Tolerisation of mice against these determinants or the use of isolated membrane lysates from (sIg -) PBL appeared promising but till now only specific thrombocyte markers have been obtained. The use of more purified antigen is recommended in further attempts. The data presented in this thesis can be used for fundamental studies on cell interactions in the immune response, but also for more applied investigations on fish health control.
Genetical and some environmental influences affecting the level of leucocyte counts in the milk of cows
Afifi, Y.A. - \ 1967
Wageningen University. Promotor(en): T. Stegenga. - Wageningen : Veenman - 81
rundvee - rauwe melk - diergeneeskunde - melkklieren - melksecretie - lactatie - dierlijke producten - vervalsing - besmetting - verouderen - gebreken - achteruitgang (deterioration) - bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - mastitis - cattle - raw milk - veterinary science - mammary glands - milk secretion - lactation - animal products - adulteration - contamination - aging - defects - deterioration - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma - mastitis
The progeny groups of different sires varied widely in white-cell count in milk, even after exclusion of all cows which had suffered from mastitis. The sire had a demonstrable effect on white-cell count in milk, especially during the second half of lactation. Heritability estimates of white-cell count in milk showed that values for the fourth lactation were higher than those for heifers. But at the end of lactation heritability values for 4th lactation cows and heifers were nearly equal (about 0.40). The daughter groups with high average white-cell counts mostly showed frequent mastitis. There was a high phenotypic and genetic correlation between clinical mastitis and white-cell count. Within seasons for cows which, so far known, had never mastitis, very high and very low producers had higher white-cell counts than other cows. White-cell counts increased remarkably with advancing lactation. A relation between white-cell count and ease of milking could not be demonstrated.Increasing milking vacuum over 40 cm mercury pressure, especially at the end of lactation, or increasing pulsation to over 50 per min. tended to increase white-cells. Milking routine (man/machine ratio) affected white-cell count in the end of lactation. More cows per milker increased the number of white-cells.
Verspreiding van bloedgroepen in het Nederlandse zwartbonte rundvee : een onderzoek naar de frequenties van bloedgroepen en naar enige factoren, die de frequenties beinvloeden
Kraaij, G.J. - \ 1967
Wageningen University. Promotor(en): T. Stegenga. - Wageningen : Veenman - 127
bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - rundvee - melkveerassen - nederland - genetica - heritability - genetische variatie - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma - cattle - dairy breeds - netherlands - genetics - heritability - genetic variation
Blood groups are genetically determined components of the red blood cells. In cattle there were 13 loci known to determine blood groups and some of these loci had large series of alleles. There were also 14 other loci known to determine proteins and enzymes in blood and milk of cattle.
The author examined how the distribution of blood groups in the Dutch Friesian population had been influenced by the restrictions of breeders. He found that parent cattle were paired independently of blood group. In offspring there was no selection for a certain phenotype until the end of the first year.
There were clear differences in the frequency of some genes between adult bulls and cows. The gene for blood group A was less frequent in bulls and that for blood group F was less frequent in cows. This occurred in some foreign breeds of cattle.
The distribution of blood groups over the population was not even. There were differences in gene frequency between breeding areas and in the breeding areas there were large differences between artificial insemination stations. Differences within farms and within breeding pedigrees were even greater. These differences could be ascribed largely to the use of one or only a few sires.
Het kopergehalte van lever en bloedserum bij het Fries-Hollandse rund
Grift, J. van der - \ 1955
's-Gravenhage : Staatsdrukkerij (Verslagen van landbouwkundige onderzoekingen 61.10) - 61
melkveerassen - rundvee - bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - dairy breeds - cattle - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma