Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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    We will mail you new results for this query: keywords==Botryococcus braunii
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Effect of removal of bacteria on the biomass and extracellular carbohydrate productivity of Botryococcus braunii
Gouveia, Joao D. ; Moers, Antoine ; Griekspoor, Yvonne ; Broek, Lambertus A.M. van den; Springer, Jan ; Sijtsma, Lolke ; Sipkema, Detmer ; Wijffels, Rene H. ; Barbosa, Maria J. - \ 2019
Journal of Applied Phycology (2019). - ISSN 0921-8971 - 11 p.
16S analysis - Bacteria - Botryococcus braunii - Chlorophyceae - Exopolysaccharides - Microalgae - UV light

Botryococcus braunii CCALA778 is a green microalga that can produce large amounts of extracellular carbohydrates and therefore is a potential host for industrial applications such as materials, food and pharmaceutical products. The downside of B. braunii is its slow growth and therefore, improvements on the biomass productivity or carbohydrate production will make this microalga more attractive for industrial exploitation. Microalgae grow naturally in the presence of bacteria and these can be beneficial or antagonistic. In outdoor cultivation systems, contamination by bacteria is common. The role or effects of bacteria present in B. braunii CCALA778 are not yet fully elucidated. We used UV-C treatment to reduce bacterial abundance in B. braunii CCALA778 cultures and 16S rRNA gene amplicon MiSeq sequencing for bacterial community analysis. The effect of the reduced amount of bacteria on biomass growth and production of extracellular carbohydrates was analysed. It is shown that UV-C treatment can reduce the bacterial population substantially without harming B. braunii. Bacteria removed by UV-C were antagonistic to B. braunii CCALA778 as in their absence production of biomass and extracellular carbohydrates was enhanced significantly. CCALA778 treated with UV-C accumulated 826 ± 61 mg L−1 of extracellular carbohydrates by day 15 compared with 422 ± 135 mg L−1 accumulated extracellular carbohydrates in the untreated culture.

Associated bacteria of Botryococcus braunii (Chlorophyta)
Gouveia, Joao D. ; Lian, Jie ; Steinert, Georg ; Smidt, Hauke ; Sipkema, Detmer ; Wijffels, Rene H. ; Barbosa, Maria J. - \ 2019
PeerJ 2019 (2019)3. - ISSN 2167-8359
16S rRNA sequencing - Algal-bacterial interactions - Associated bacteria - Botryococcus braunii

Botryococcus braunii (Chlorophyta) is a green microalga known for producing hydrocarbons and exopolysaccharides (EPS). Improving the biomass productivity of B. braunii and hence, the productivity of the hydrocarbons and of the EPS, will make B. braunii more attractive for industries. Microalgae usually cohabit with bacteria which leads to the formation of species-specific communities with environmental and biological advantages. Bacteria have been found and identified with a few B. braunii strains, but little is known about the bacterial community across the different strains. A better knowledge of the bacterial community of B. braunii will help to optimize the biomass productivity, hydrocarbons, and EPS accumulation. To better understand the bacterial community diversity of B. braunii, we screened 12 strains from culture collections. Using 16S rRNA gene analysis by MiSeq we described the bacterial diversity across 12 B. braunii strains and identified possible shared communities. We found three bacterial families common to all strains: Rhizobiaceae, Bradyrhizobiaceae, and Comamonadaceae. Additionally, the results also suggest that each strain has its own specific bacteria that may be the result of long-term isolated culture.

Effects of hydrostatic pressure and supercritical carbon dioxide on the viability of Botryococcus braunii algae cells
Yildiz-Ozturk, Ece ; Ilhan-Ayisigi, Esra ; Togtema, Arnoud ; Gouveia, Joao ; Yesil-Celiktas, Ozlem - \ 2018
Bioresource Technology 256 (2018). - ISSN 0960-8524 - p. 328 - 332.
Botryococcus braunii - Cell viability - Hydrostatic pressure - Recultivation - Supercritical CO
In bio-based industries, Botryococcus braunii is identified as a potential resource for production of hydrocarbons having a wide range of applications in chemical and biopolymer industries. For a sustainable production platform, the algae cultivation should be integrated with downstream processes. Ideally the algae are not harvested, but the product is isolated while cultivation and growth is continued especially if the doubling time is slow. Consequently, hydrocarbons can be extracted while keeping the algae viable. In this study, the effects of pressure on the viability of B. braunii cells were tested hydrostatically and under supercritical CO2 conditions. Viability was determined by light microscopy, methylene blue uptake and by re-cultivation of the algae after treatments to follow the growth. It was concluded that supercritical CO2 was lethal to the algae, whereas hydrostatic pressure treatments up to 150 bar have not affected cell viability and recultivation was successful.
Identification of methylated GnTI-dependent N-glycans in Botryococcus brauni
Schulze, Stefan ; Urzica, Eugen ; Reijnders, Maarten J.M.F. ; Geest, Henri van de; Warris, Sven ; Bakker, Linda V. ; Fufezan, Christian ; Martins dos Santos, Vitor A.P. ; Schaap, Peter J. ; Peters, Sander A. ; Hippler, Michael - \ 2017
New Phytologist 215 (2017)4. - ISSN 0028-646X - p. 1361 - 1369.
Botryococcus braunii - gene ontology annotation - mass spectrometry - N-glycosylation - post-translational modification
In contrast to mammals and vascular plants, microalgae show a high diversity in the N-glycan structures of complex N-glycoproteins. Although homologues for β1,2-N-acetylglucosaminyltransferase I (GnTI), a key enzyme in the formation of complex N-glycans, have been identified in several algal species, GnTI-dependent N-glycans have not been detected so far. We have performed an N-glycoproteomic analysis of the hydrocarbon oils accumulating green microalgae Botryococcus braunii. Thereby, the analysis of intact N-glycopeptides allowed the determination of N-glycan compositions. Furthermore, insights into the role of N-glycosylation in B. braunii were gained from functional annotation of the identified N-glycoproteins. In total, 517 unique N-glycosylated peptides have been identified, including intact N-glycopeptides that harbored N-acetylhexosamine (HexNAc) at the nonreducing end. Surprisingly, these GnTI-dependent N-glycans were also found to be modified with (di)methylated hexose. The identification of GnTI-dependent N-glycans in combination with N-glycan methylation in B. braunii revealed an uncommon type of N-glycan processing in this microalgae.
Metagenomic analysis of the complex microbial consortium associated with cultures of the oil-rich alga Botryococcus braunii
Sambles, Christine ; Moore, Karen ; Lux, Thomas M. ; Jones, Katy ; Littlejohn, George R. ; Gouveia, João D. ; Aves, Stephen J. ; Studholme, David J. ; Lee, Rob ; Love, John - \ 2017
MicrobiologyOpen 6 (2017)4. - ISSN 2045-8827
Botryococcus braunii - Biofuel - Consortium - Metagenomics - Microcosm
Microalgae are widely viewed as a promising and sustainable source of renewable chemicals and biofuels. Botryococcus braunii synthesizes and secretes significant amounts of long-chain (C30-C40) hydrocarbons that can be subsequently converted into gasoline, diesel, and aviation fuel. B. braunii cultures are not axenic and the effects of co-cultured microorganisms on B. braunii growth and hydrocarbon yield are important, but sometimes contradictory. To understand the composition of the B. braunii microbial consortium, we used high throughput Illumina sequencing of metagenomic DNA to profile the microbiota within a well established, stable B. braunii culture and characterized the demographic changes in the microcosm following modification to the culture conditions. DNA sequences attributed to B. braunii were present in equal quantities in all treatments, whereas sequences assigned to the associated microbial community were dramatically altered. Bacterial species least affected by treatments, and more robustly associated with the algal cells, included members of Rhizobiales, comprising Bradyrhizobium and Methylobacterium, and representatives of Dyadobacter, Achromobacter and Asticcacaulis. The presence of bacterial species identified by metagenomics was confirmed by additional 16S rDNA analysis of bacterial isolates. Our study demonstrates the advantages of high throughput sequencing and robust metagenomic analyses to define microcosms and further our understanding of microbial ecology.
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