Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Dataset for the annotations of the transcriptome of the hemp cultivar C. sativa ‘Finola’ (finola1) and the marihuana cultivar C. sativa ‘Purple Kush’
    Petit Pedro, Jordi ; Salentijn, Elma ; Loo, Robert van; Caldas Paulo, Joao ; Denneboom, Christel ; Trindade, Luisa - \ 2020
    Wageningen University & Research
    GWAS - Cannabis sativa - hemp - fibre quality - cell wall composition - RAD-sequencing - QTL - sex determination - flowering time - plant breeding
    This dataset contains the annotations of the two transcriptomes of the hemp cultivar C. sativa ‘Finola’ (finola1) and the marihuana cultivar C. sativa ‘Purple Kush’, aligned to the C. sativa ‘Purple Kush’ (canSat3 version GAC 000230575.1) assembly from the C. sativa Genome Browser Gateway http://genome.ccbr.utoronto.ca/cgi-bin/hgGateway (van Bakel et al., 2011). The annotations were performed using Local Blast and the data is provided in gff files. Gff means General Feature Format and it's a simple tab-delimited text file for describing genomic features. The files are ready to be uploaded in a genomic viewer.
    Dataset of the RADseq data and genotyping from a panel of 123 hemp accessions
    Petit Pedro, Jordi ; Salentijn, Elma ; Loo, Robert van; Caldas Paulo, Joao ; Denneboom, Christel ; Trindade, Luisa - \ 2020
    Wageningen University and Research
    GWAS - Cannabis sativa - hemp - fibre quality - cell wall composition - RAD-sequencing - QTL - sex determination - flowering time - plant breeding
    This dataset contains the raw RADseq data and genotyping data from a panel of 123 hemp (Cannabis sativa L.) accessions. For extra information about the RAD sequencing data analysis and the SNP marker selection, read the materials and methods of the paper 'Elucidating the genetic architecture of fibre quality in hemp (Cannabis sativa L.) using the Genome-Wide Association Study'.
    Genetic Variability of Morphological, Flowering, and Biomass Quality Traits in Hemp (Cannabis sativa L.)
    Petit, Jordi ; Salentijn, Elma M.J. ; Paulo, Maria João ; Thouminot, Claire ; Dinter, Bert Jan van; Magagnini, Gianmaria ; Gusovius, Hans Jörg ; Tang, Kailei ; Amaducci, Stefano ; Wang, Shaoliang ; Uhrlaub, Birgit ; Müssig, Jörg ; Trindade, Luisa M. - \ 2020
    Frontiers in Plant Science 11 (2020). - ISSN 1664-462X
    Cannabis sativa - cell wall composition - fiber quality - flowering time - genetic variability - genotype-by-environment (G×E) interactions - hemp - sex determination

    Hemp (Cannabis sativa L.) is a bast-fiber crop well-known for the great potential to produce sustainable fibers. Nevertheless, hemp fiber quality is a complex trait, and little is known about the phenotypic variability and heritability of fiber quality traits in hemp. The aim of this study is to gain insights into the variability in fiber quality within the hemp germplasm and to estimate the genetic components, environmental components, and genotype-by-environment (G×E) interactions on fiber quality traits in hemp. To investigate these parameters, a panel of 123 hemp accessions was phenotyped for 28 traits relevant to fiber quality at three locations in Europe, corresponding to climates of northern, central, and southern Europe. In general, hemp cultivated in northern latitudes showed a larger plant vigor while earlier flowering was characteristic of plants cultivated in southern latitudes. Extensive variability between accessions was observed for all traits. Most cell wall components (contents of monosaccharides derived from cellulose and hemicellulose; and lignin content), bast fiber content, and flowering traits revealed large genetic components with low G×E interactions and high broad-sense heritability values, making these traits suitable to maximize the genetic gains of fiber quality. In contrast, contents of pectin-related monosaccharides, most agronomic traits, and several fiber traits (fineness and decortication efficiency) showed low genetic components with large G×E interactions affecting the rankings across locations. These results suggest that pectin, agronomic traits, and fiber traits are unsuitable targets in breeding programs of hemp, as their large G×E interactions might lead to unexpected phenotypes in untested locations. Furthermore, all environmental effects on the 28 traits were statistically significant, suggesting a strong adaptive behavior of fiber quality in hemp to specific environments. The high variability in fiber quality observed in the hemp panel, the broad range in heritability, and adaptability among all traits prescribe positive prospects for the development of new hemp cultivars of excellent fiber quality.

    Phenotypic Variation of Cell Wall Composition and Stem Morphology in Hemp (Cannabis sativa L.): Optimization of Methods
    Petit, Jordi ; Gulisano, Agata ; Dechesne, Annemarie ; Trindade, Luisa M. - \ 2019
    Frontiers in Plant Science 10 (2019). - ISSN 1664-462X
    Cannabis sativa - cell wall - fiber quality - genetic diversity - hemp - phenotyping methods - stem morphology

    The growing demands for sustainable fibers have stimulated the study of genetic diversity in the quality of hemp fiber (Cannabis sativa L.). Nevertheless, the lack of high-throughput phenotyping methods that are suited for the analysis of hemp fiber, hampers the analysis of many accessions, and consequently the breeding for this complex trait. In the present report, we developed and optimized the throughput of five methods to study the diversity in hemp fiber quality including cell wall extraction, biochemical composition of cell wall polysaccharides, quantification of lignin, quantification of crystalline polysaccharides and morphology of the stems. Six hemp accessions contrasting for cell wall properties were used to assess the throughput and suitability of these methods for genetic studies. The methods presented revealed to be highly repeatable, with low coefficients of variation between technical replicates. With these methods we were able to detect significant phenotypic variation in cell wall composition and stem morphology between the six accessions. In addition, the throughput of the methods has been upgraded to a level that enables their use for phenotyping cell wall traits in breeding programs. The cell wall extraction was optimized to extract enough material for the complete characterization of the cell wall of hemp while reducing the time for the entire analysis. The throughput of the stem morphological analysis was improved by decreasing the timing of fixation, infiltration, and embedding of mature and dry hemp stems. Notwithstanding, our methods already have the potential to phenotype large number of accessions in a relatively short period of time. Our methods will enable exploration of genetic diversity of fiber quality and will contribute to the development of new hemp varieties with advanced quality of fibers.

    The complex interactions between flowering behavior and fiber quality in hemp
    Salentijn, Elma M.J. ; Petit, Jordi ; Trindade, Luisa M. - \ 2019
    Frontiers in Plant Science 10 (2019). - ISSN 1664-462X
    Cannabis sativa - Fiber development - Flowering-time - Hemp - Phenology - Sex determination - Short-day plant

    Hemp, Cannabis sativa L., is a sustainable multipurpose fiber crop with high nutrient and water use efficiency and with biomass of excellent quality for textile fibers and construction materials. The yield and quality of hemp biomass are largely determined by the genetic background of the hemp cultivar but are also strongly affected by environmental factors, such as temperature and photoperiod. Hemp is a facultative short-day plant, characterized by a strong adaptation to photoperiod and a great influence of environmental factors on important agronomic traits such as “flowering-time” and “sex determination.” This sensitivity of hemp can cause a considerable degree of heterogeneity, leading to unforeseen yield reductions. Fiber quality for instance is influenced by the developmental stage of hemp at harvest. Also, male and female plants differ in stature and produce fibers with different properties and quality. Next to these causes, there is evidence for specific genotypic variation in fiber quality among hemp accessions. Before improved hemp cultivars can be developed, with specific flowering-times and fiber qualities, and adapted to different geographical regions, a better understanding of the molecular mechanisms controlling important phenological traits such as “flowering-time” and “sex determination” in relation to fiber quality in hemp is required. It is well known that genetic factors play a major role in the outcome of both phenological traits, but the major molecular factors involved in this mechanism are not characterized in hemp. Genome sequences and transcriptome data are available but their analysis mainly focused on the cannabinoid pathway for medical purposes. Herein, we review the current knowledge of phenotypic and genetic data available for “flowering-time,” “sex determination,” and “fiber quality” in short-day and dioecious crops, respectively, and compare them with the situation in hemp. A picture emerges for several controlling key genes, for which natural genetic variation may lead to desired flowering behavior, including examples of pleiotropic effects on yield quality and on carbon partitioning. Finally, we discuss the prospects for using this knowledge for the molecular breeding of this sustainable crop via a candidate gene approach.

    Vezelhennep als papiergrondstof : teeltonderzoek 1990 - 1993 = Fibre hemp as a raw material for paper : agronomic research 1990 - 1993
    Werf, H.M.G. van der; Geel, W.C.A. van - \ 1994
    Lelystad : PAGV (Verslag / Proefstation voor de Akkerbouw en de Groenteteelt in de Vollegrond nr. 177) - 62
    Cannabis sativa - bordpapier - hennep - industrie - papier - karton - pulp- en papierwarenindustrie - textielvezels - Cannabis sativa - cardboard - hemp - industry - paper - paperboard - pulp and paper industry - textile fibres
    Afzetperspectieven voor Nederlandse hennep en henneppulp in de papiersector
    Onna, M.J.G. van; Ent, E.J. van den - \ 1994
    Den Haag : Landbouw-Economisch Instituut (LEI-DLO) (Onderzoekverslag / Landbouw-Economisch Instituut (LEI-DLO), Afdeling Landbouw 118) - ISBN 9789052422367 - 103
    Cannabis sativa - hennep - ruwe grondstoffen - papier - plantenvezels - bosbouw - pulp- en papierwarenindustrie - cellulose - cellulosevezels - handel - markten - marketing - marktconcurrentie - aanbodsevenwicht - Nederland - Cannabis sativa - hemp - raw materials - paper - plant fibres - forestry - pulp and paper industry - cellulose - cellulosic fibres - trade - markets - marketing - market competition - supply balance - Netherlands
    De geschiktheid van de bodem van Nederland voor de teelt van Cannabis sativa (hennep) en Miscanthus sinensis 'Giganteus'.
    Soesbergen, G.A. van; Lanen, H.A.J. van - \ 1993
    Wageningen : DLO-Staring Centrum - 59
    Cannabis sativa - vezelgewassen - hennep - landevaluatie - Nederland - bodemgeschiktheid - Cannabis sativa - fibre plants - hemp - land evaluation - Netherlands - soil suitability
    Teelt en verwerking van vlas en hennep in de Sovjet-Unie en Polen : verslag van een studiereis in september 1964 en mei 1965
    Friederich, J.C. ; Sparenberg, H. - \ 1967
    Wageningen : [s.n.] (Mededeling / Proefstation voor de akker- en weidebouw no. 129) - 47
    Cannabis sativa - teelt - cultuurmethoden - vlas - hennep - linnen - Linum usitatissimum - Polen - textielvezels - textielindustrie - USSR in Europa - Cannabis sativa - cultivation - cultural methods - flax - hemp - linen - Linum usitatissimum - Poland - textile fibres - textile industry - USSR in Europe
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