Optical trapping in plant cells
Ketelaar, Tijs ; Ruijter, Norbert de; Niehren, Stefan - \ 2019
In: Methods in Molecular Biology / Cvrcková, F., Zárský, V., Hertfordshire : Springer (Methods in Molecular Biology ) - ISBN 9781493994687 - p. 231 - 238.
Confocal microscope - Cytoskeleton - Endomembrane system - Noninvasive manipulation - Optical trap - Optical tweezers - Plant cell
Optical tweezers allow for noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope. Next, we describe experimental approaches we have taken to trap intracellular structures in plant cells.