- Faline D.M. Plantenga (1)
- Leo F.M. Marcelis (1)
- Martijn Fiers (1)
- Richard G.F. Visser (1)
- Richard G.H. Immink (1)
- E. Heuvelink (1)
- Ep Heuvelink (1)
- Jorin Hoogenboom (1)
- L.F.M. Marcelis (1)
- F.D.M. Plantenga (1)
- M. Siakou (1)
- R.G.F. Visser (1)
- Christian W.B. Bachem (1)
- Tom Wennekes (1)
High light accelerates potato flowering independently of the FT-like flowering signal StSP3D
Plantenga, Faline D.M. ; Bergonzi, Sara ; Bachem, Christian W.B. ; Visser, Richard G.F. ; Heuvelink, Ep ; Marcelis, Leo F.M. - \ 2019
Environmental and Experimental Botany 160 (2019). - ISSN 0098-8472 - p. 35 - 44.
Daily light integral (DLI) - Flowering - Potato - StSP3D - StTPS1 - Sucrose
Little is known on the environmental control of potato flowering. With recent developments in potato breeding, and the growing interest in true potato seed production, more knowledge on potato flowering is required. This research aims to elucidate the effect of the daily light integral (DLI: the accumulated light throughout a day) on potato flower initiation time and investigate which mechanisms underlie this control. We grew potato plants in climate chambers to compare flower initiation under different DLIs in short and long days. We measured the time until the first appearance of the flower buds and the number of leaves formed before the inflorescence. Furthermore, gene expression changes of the potato flowering time gene StSP3D were measured, and potato plants silenced in StSP3D were used to determine whether DLI mediated flowering time was regulated through StSP3D. Additionally, we determined sucrose and starch concentrations and measured the transcription levels of StTPS1, a gene involved in sugar mediated flowering control. Increasing DLI clearly accelerated flowering in potato. The role of carbohydrates (sucrose and starch) and StTPS1 in DLI-accelerated flowering was inconclusive. Although StSP3D was upregulated under high DLI, transgenic lines silenced in StSP3D also showed accelerated flowering under higher DLIs. We therefore conclude that high DLI accelerates potato flowering and this acceleration happens independently of StSP3D upregulation.
A plant-based chemical genomics screen for the identification of flowering inducers
Fiers, Martijn ; Hoogenboom, Jorin ; Brunazzi, Alice ; Wennekes, Tom ; Angenent, Gerco C. ; Immink, Richard G.H. - \ 2017
Plant Methods 13 (2017). - ISSN 1746-4811 - 9 p.
APETALA1 - Arabidopsis - Chemical genomics - Flowering - Luciferase - Salicylic acid
Background: Floral timing is a carefully regulated process, in which the plant determines the optimal moment to switch from the vegetative to reproductive phase. While there are numerous genes known that control flowering time, little information is available on chemical compounds that are able to influence this process. We aimed to discover novel compounds that are able to induce flowering in the model plant Arabidopsis. For this purpose we developed a plant-based screening platform that can be used in a chemical genomics study. Results: Here we describe the set-up of the screening platform and various issues and pitfalls that need to be addressed in order to perform a chemical genomics screening on Arabidopsis plantlets. We describe the choice for a molecular marker, in combination with a sensitive reporter that's active in plants and is sufficiently sensitive for detection. In this particular screen, the firefly Luciferase marker was used, fused to the regulatory sequences of the floral meristem identity gene APETALA1 (AP1), which is an early marker for flowering. Using this screening platform almost 9000 compounds were screened, in triplicate, in 96-well plates at a concentration of 25μM. One of the identified potential flowering inducing compounds was studied in more detail and named Flowering1 (F1). F1 turned out to be an analogue of the plant hormone Salicylic acid (SA) and appeared to be more potent than SA in the induction of flowering. The effect could be confirmed by watering Arabidopsis plants with SA or F1, in which F1 gave a significant reduction in time to flowering in comparison to SA treatment or the control. Conclusions: In this study a chemical genomics screening platform was developed to discover compounds that can induce flowering in Arabidopsis. This platform was used successfully, to identify a compound that can speed-up flowering in Arabidopsis.
Regulating flower and tuber formation in potato with light spectrum and day length
Plantenga, F.D.M. ; Siakou, M. ; Bergonzi, S. ; Heuvelink, E. ; Bachem, C.W.B. ; Visser, R.G.F. ; Marcelis, L.F.M. - \ 2016
Acta Horticulturae 1134 (2016). - ISSN 0567-7572 - p. 267 - 275.
Blue light - Far-red light - Flowering - LED - Photoperiod - Tuberization
Solanum tuberosum (potato) can reproduce through tubers and through seeds. Recent developments have enabled hybrid breeding and propagation from seeds in this crop. This makes potato flowering a new focus of research interest. Tuberization and presumably flowering, followed by seed set, are strongly regulated by environmental cues. A well-studied environmental regulator of tuber formation is day length. Photoreceptors are involved in this photoperiodic control of tuberization, suggesting light spectrum may be an important factor for tuberization. However, it is not known how photoreceptors control potato flowering. Here, we aim to elucidate the influence of light spectrum and photoperiod on tuber and flower formation, by growing three potato genotypes in climate chambers with light-emitting diode (LED) lighting and additional far-red and blue LEDs under long and short days. Far-red light accelerated tuber formation up to eleven days and blue light slightly delayed it up to four days. An effect of light spectrum on flowering was not found. Long photoperiods delayed tuber formation compared to short-day conditions in two of the three tested genotypes. Aside from one genotype which only flowered in long-days, no effect of photoperiod on flowering was found.