- M. Bink(older publications) (1)
- M.C.A.M. Bink (1)
- Gerhard Buck-Sorlin (1)
- N. Busatto (1)
- F. Costa (2)
- W.E. De Weg Van (1)
- M. Delledonne (1)
- Anja Dieleman (1)
- T. Dueck (1)
- Julienne Fanwoua (1)
- B. Farneti (1)
- M. Guardo Di (1)
- M. Guardo Di (1)
- W. Guerra (2)
- Michel Génard (1)
- C. Labrie (1)
- T. Letschka (1)
- L. Lozano (2)
- A. Tadiello (1)
- R. Velasco(older publications) (1)
- R. Velasco (1)
- Gilles Vercambre (1)
- W. Verkerke (1)
- Pieter Visser de (1)
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Genome wide association studies and whole transcriptomic survey decipher the fruit texture regulation in apple towards the selection of novel superior accessions
Guardo, M. Di; Tadiello, A. ; Farneti, B. ; Busatto, N. ; Delledonne, M. ; Guerra, W. ; Letschka, T. ; Lozano, L. ; Velasco, R. ; Weg, E. Van de; Bink, M. ; Costa, F. - \ 2019
Acta Horticulturae 1242 (2019). - ISSN 0567-7572 - p. 441 - 446.
Ethylene - Fruit quality - GWAS - Marker assisted selection - Pedigree based analysis - Texture
Fruit quality is represented by a series of genetically controlled features that change throughout the entire ontogenic life. Among the several quality traits, texture plays a crucial role, impacting both consumers’ appreciation and postharvest performance. In order to decipher its regulation a multidisciplinary approach was employed. Initially, the texture performance was measured with a high resolution phenotyping device, represented by a texture analyzer equipped with an acoustic device. In the first attempt to dissect the fruit texture genetic control, two QTL mapping strategies were used. The first approach employed six bi-parental families linked by a common pedigree scheme, known as pedigree based analysis. The joint analysis of the phenotypic and genotypic data set through a Bayesian statistics identified a series of genomic regions related to both mechanical and acoustic signatures. These regions were further validated with a genome wide association study approach, which considered a much larger phenotypic and genotypic variation. To complement the genetic information, a whole transcriptome analysis was also carried out. To this end, two microarray platforms were designed and used to unravel the functional machinery ongoing during the fruit development and ripening phases, especially with regards to the plant hormone ethylene. In this study, the role of this hormone was dissected applying 1-MCP, a molecule competing with ethylene at receptor level. The combination of these resources provides a valuable source of information, essential to step forward in the comprehension of the genetic and physiological regulation of the fruit texture in apple. This knowledge would enable, in a close future, a more accurate and precise selection of the most favourable and valuable new apple accessions distinguished by a superior fruit quality.
Supplemental LED lighting affects the dynamics of tomato fruit growth and composition
Fanwoua, Julienne ; Vercambre, Gilles ; Buck-Sorlin, Gerhard ; Dieleman, Anja ; Visser, Pieter de; Génard, Michel - \ 2019
Scientia Horticulturae 256 (2019). - ISSN 0304-4238
Acid - Dilution - Fruit quality - Light-emitting diodes - Metabolism - Sugar - Tomato fruit
Understanding how greenhouse crops respond to supplemental lighting with light-emitting diodes (LEDs) compared with traditional lighting systems is essential to utilize the full potential of LEDs and their further adoption in energy efficient greenhouses. This study quantified the effects of supplemental lighting with high-pressure sodium (HPS) lamps and LED light on the dynamics of fruit growth and composition in tomato (Solanum lycopersicum L.). Two tomato genotypes (‘Foundation’ and ‘Progression’) were grown under daylight supplemented either with HPS (125 μmol m−2 s−1) combined with red/blue LED lighting (106 μmol m−2 s−1, HPS + LED light treatment) or red/blue LED light only (106 + 110 μmol m−2 s−1, LED + LED light treatment); and two genotypes (‘Foundation’ and ‘NUN09204’) under daylight supplemented either with red/blue LED light (200 μmol m−2 s−1, red/blue LED light treatment) or red/blue LED + far-red LED light (200 μmol m−2 s−1 + 40 μmol m−2 s−1, red/blue + far-red LED light treatment). Fresh weight and composition in glucose, fructose, sucrose, starch, citric acid and malic acid of tomato fruits at different stages of development were measured and analyzed in terms of three main underlying components: water dilution, dilution by soluble and storage compounds and metabolism. Growing fruits under the LED + LED compared to the HPS + LED light treatments increased average fruit fresh weight in all genotypes. The red/blue + far-red LED light treatment increased the production of soluble sugar, increased the dilution by soluble and storage compounds, and reduced water dilution leading to a strong increase in glucose, fructose and sucrose concentration in the pericarp. The LED + LED light treatment did not affect the metabolism of fruit biochemical compounds compared to the HPS + LED light treatments, but caused small changes in water dilution, which were reflected in the concentration of biochemical compounds. Dilution and metabolism were involved in genotypic differences in fruit composition. Our results show that altering the spectral composition of the supplemental light in energy efficient greenhouses can be done without an effect on fruit quality or even with an improvement of tomato fruit quality. Possible physiological processes underlying these light-induced changes in fruit biochemical compounds during fruit development in different genotypes were discussed.
A joint Laimburg - FEM molecular markers project for apple fruit quality traits using the Pedigree Based Analysis strategy
Lozano, L. ; Guardo, M. Di; De Weg, W.E. Van; Bink, M.C.A.M. ; Velasco, R. ; Costa, F. ; Guerra, W. - \ 2015
In: III International Symposium on Molecular Markers in Horticulture / Velasco, R., International Society for Horticultural Science (Acta Horticulturae ) - ISBN 9789462610989 - p. 91 - 94.
Fruit quality - Malus ×domestica - Markerassisted selection - Pedigree Based Analysis - QTLs
In apple breeding fruit quality is a crucial aspect to guarantee commercial success of new varieties. Among the several quality attributes, individual sugars and acid components, as well as texture, are considered important features due to their significant contribution to sensory quality and consumer's acceptance. In traditional breeding, however, selection for these particular traits is a time consuming process, due to the long unproductive juvenile phase of apple seedlings. This step can be assisted to date by the use of molecular markers, an important diagnostic tool useful to anticipate and assist the identification of novel apple varieties characterized by superior fruit quality behaviour. However, the routinely implementation of molecular markers in breeding programs is still limited. In this study a new approach named Pedigree Based Analysis will be used on six full-sib progenies and their pedigree (composed by 15 varieties, represented by founders and ancestors), to target the main set of quantitative trait loci (QTLs). This strategy will allow the identification of the most valuable alleles present in the breeding activities of both Italian institutes. The ultimate goal of the Laimburg - FEM joint project is the identification and validation of molecular markers as a suitable tool for Marker Assisted Breeding in the two on-going apple breeding programs.
The effect of light intensity and duration on Vitamin C concentration in tomato fruits
Verkerke, W. ; Labrie, C. ; Dueck, T. - \ 2015
Acta Horticulturae 1106 (2015). - ISSN 0567-7572 - p. 49 - 53.
Artificial lighting - Ascorbic acid - Fruit quality - Health related phytochemicals secondary metabolites - Led - Solanum lycopersicum
Increasing health related phytochemicals (HRP) in fruits may create an added value for consumers. We are currently developing greenhouse cultivation protocols for growers to fine tune plant growth and create products with higher amounts of HRP. Tomato (Solanum lycopersicum) greenhouse experiments were carried out to investigate the effect of light intensity and light quality on HRP during the growing period. Vitamin C concentration of different tomato cultivars was increased in a model growing system with red and blue light-emitting diodes (LEDs), increasing logarithmically with increasing radiation. At moderate light intensities of 140, 200 and 285 μmol m-2 s-1 and a light sum of 10-30 mol day-1, the Vitamin C concentration increased by 65% in comparison to the controls. The light sum is best applied at low intensity for a longer period (20 h) than at higher intensities for a shorter period (14 h). The total amount of antioxidants, estimated as oxygen radical absorbance capacity (ORAC) values, also increased. With these results, practical cultivation systems can be developed to increase the Vitamin C concentration, enabling growers to give their products an added value.