- Paweł Bednarek (1)
- Olivier Bouchez (1)
- Stéphanie Boutet-Mercey (1)
- Ted C.J. Turlings (1)
- Hervé Canut (1)
- Gang Chen (1)
- Chiara Consonni (1)
- Marcel Dicke (2)
- Rocío Escobar-Bravo (1)
- Ya Fen Zhang (1)
- Maite Fernández Bobadilla de (1)
- Ivo Feussner (1)
- Julia Friman (1)
- Peter G.L. Klinkhamer (1)
- Anne Gouget (1)
- Francine Govers (1)
- Nathalie Haget (1)
- Joop J.A. Loon van (1)
- Peng Jun Zhang (1)
- Hannah Kuhn (1)
- Mark Kwaaitaal (1)
- Hye Kyong Kim (1)
- Justine Lorek (1)
- Tom M. Raaymakers (1)
- Cristina Micali (1)
- Jia Ning Wei (1)
- Nurmi Pangesti (1)
- Ana Pineda Gomez (1)
- Xiao Ping Yu (1)
- Dominique Roby (1)
- Shu Sheng Liu (1)
- Camille Souriac (1)
- Emiel Ver Loren Themaat Van (1)
- Chuan You Li (1)
- Chan Zhao (1)
Airborne host–plant manipulation by whiteflies via an inducible blend of plant volatiles
Zhang, Peng Jun ; Wei, Jia Ning ; Zhao, Chan ; Zhang, Ya Fen ; Li, Chuan You ; Liu, Shu Sheng ; Dicke, Marcel ; Yu, Xiao Ping ; Turlings, Ted C.J. - \ 2019
Proceedings of the National Academy of Sciences of the United States of America 116 (2019)15. - ISSN 0027-8424 - p. 7387 - 7396.
Herbivore-induced plant volatiles - Jasmonic acid - Salicylic acid - Tomato - Whiteflies
The whitefly Bemisia tabaci is one of the world’s most important invasive crop pests, possibly because it manipulates plant defense signaling. Upon infestation by whiteflies, plants mobilize salicylic acid (SA)-dependent defenses, which mainly target pathogens. In contrast, jasmonic acid (JA)-dependent defenses are gradually suppressed in whitefly-infested plants. The down-regulation of JA defenses make plants more susceptible to insects, including whiteflies. Here, we report that this host–plant manipulation extends to neighboring plants via airborne signals. Plants respond to insect attack with the release of a blend of inducible volatiles. Perception of these volatiles by neighboring plants usually primes them to prepare for an imminent attack. Here, however, we show that whitefly-induced tomato plant volatiles prime SA-dependent defenses and suppress JA-dependent defenses, thus rendering neighboring tomato plants more susceptible to whiteflies. Experiments with volatiles from caterpillar-damaged and pathogen-infected plants, as well as with synthetic volatiles, confirm that whiteflies modify the quality of neighboring plants for their offspring via whitefly-inducible plant volatiles.
Induced resistance against western flower thrips by the pseudomonas syringae-derived defense elicitors in tomato
Chen, Gang ; Escobar-Bravo, Rocío ; Kim, Hye Kyong ; Leiss, Kirsten A. ; Klinkhamer, Peter G.L. - \ 2018
Frontiers in Plant Science 9 (2018). - ISSN 1664-462X
Coronatine - Frankliniella occidentalis - Induced plant defenses - Jasmonic acid - Pseudomonas syringae - Salicylic acid - Solanum lycopersicum - Type VI glandular trichomes
Western flower thrips (WFT) Frankliniella occidentalis (Pergande) is a key agricultural pest of cultivated tomatoes. Induced host plant resistance by activating jasmonic acid (JA) signaling pathway constitutes a promising method for WFT control. The phytotoxin coronatine (COR), produced by Pseudomonas syringae pv. tomato DC3000 (Pst), mimics the plant hormone JA-Isoleucine and can promote resistance against herbivorous arthropods. Here we determined the effect of Pst and COR on tomato resistance against WFT, induction of JA and salicylic acid (SA) associated defenses, and plant chemistry. Additionally, we investigated the presence of other components in Pst-derived and filtered culture medium, and their interactive effect with COR on tomato resistance to WFT. Our results showed that infiltration of COR or Pst reduced WFT feeding damage in tomato plants. COR and Pst induced the expression of JA-associated gene and protein marker. COR also induced expression of a SA-related responsive gene, although at much less magnitude. Activation of JA defenses in COR and Pst infiltrated plants did not affect density of type VI leaf trichomes, which are defenses reported to be induced by JA. An untargeted metabolomic analysis showed that both treatments induced strong changes in infiltrated leaves, but leaf responses to COR or Pst slightly differed. Application of the Pst-derived and filtered culture medium, containing COR but not viable Pst, also increased tomato resistance against WFT confirming that the induction of tomato defenses does not require a living Pst population to be present in the plant. Infiltration of tomato plants with low concentrations of COR in diluted Pst-derived and filtered culture medium reduced WFT feeding damage in a greater magnitude than infiltration with an equivalent amount of pure COR indicating that other elicitors are present in the medium. This was confirmed by the fact that the medium from a COR-mutant of Pst also strongly reduced silver damage. In conclusion, our results indicate that induction of JA defenses by COR, Pst infection, the medium of Pst and the medium of a Pst COR- mutant increased resistance against WFT. This was not mediated by the reinforcement of leaf trichome densities, but rather the induction of chemical defenses.
Does drought stress modify the effects of plant-growth promoting rhizobacteria on an aboveground chewing herbivore?
Bobadilla, Maite Fernández de; Friman, Julia ; Pangesti, Nurmi ; Dicke, Marcel ; Loon, Joop J.A. van; Pineda Gomez, Ana - \ 2017
Insect Science 24 (2017)6. - ISSN 1672-9609 - p. 1034 - 1044.
Abiotic stress - Abscisic acid - Crosstalk - Induced systemic resistance - Jasmonic acid - Pseudomonas simiae
Soil microbes have important effects on the interactions of plants with their environment, by promoting plant growth, inducing resistance to pests or by conferring tolerance to abiotic stress. However, their effects are variable and the factors responsible for this variation are mainly unknown. Our aim was to assess how drought stress modifies the effect of the nonpathogenic rhizobacterium Pseudomonas simiae WCS417r on plant growth and resistance against the generalist leaf-chewing caterpillar Mamestra brassicae. We studied Arabidopsis thaliana Col-0 plants, as well as mutants altered in the biosynthesis of the phytohormones jasmonic acid (JA) and abscisic acid (ABA). Caterpillars did not prefer rhizobacteria-treated plants, independently of drought stress. Rhizobacteria colonization had a variable effect on caterpillar performance, which ranged from positive in one experiment to neutral in a second one. Drought had a consistent negative effect on herbivore performance; however, it did not modify the effect of rhizobacteria on herbivore performance. The effect of drought on herbivore performance was JA-mediated (confirmed with the use of the dde2-2 mutant), but it was still present in the ABA-deficient mutant aba2-1. Plant biomass was reduced by both drought and herbivory but it was enhanced by rhizobacterial colonization. Pseudomonas simiae WCS417r is able to promote plant growth even when plants are suffering herbivory. Nevertheless, the microbial effect on the herbivore is variable, independently of drought stress. To get the best possible outcome from the rhizobacteria-plant mutualism it is important to understand which other factors may be responsible for its context-dependency.
Key components of different plant defense pathways are dispensable for powdery mildew resistance of the arabidopsis mlo2 mlo6 mlo12 triple mutant
Kuhn, Hannah ; Lorek, Justine ; Kwaaitaal, Mark ; Consonni, Chiara ; Becker, Katia ; Micali, Cristina ; Themaat, Emiel Ver Loren Van; Bednarek, Paweł ; Raaymakers, Tom M. ; Appiano, Michela ; Bai, Yuling ; Feussner, Ivo - \ 2017
Frontiers in Plant Science 8 (2017). - ISSN 1664-462X
Camalexin - Indole glucosinolates - Jasmonic acid - Microarray analysis - MLO - Plant defense - Powdery mildew - Tryptophan
Loss of function mutations of particular plant MILDEW RESISTANCE LOCUS O (MLO) genes confer durable and broad-spectrum penetration resistance against powdery mildew fungi. Here, we combined genetic, transcriptomic and metabolomic analyses to explore the defense mechanisms in the fully resistant Arabidopsis thaliana mlo2 mlo6 mlo12 triple mutant. We found that this genotype unexpectedly overcomes the requirement for indolic antimicrobials and defense-related secretion, which are critical for incomplete resistance of mlo2 single mutants. Comparative microarray-based transcriptome analysis of mlo2 mlo6 mlo12 mutants and wild type plants upon Golovinomyces orontii inoculation revealed an increased and accelerated accumulation of many defense-related transcripts. Despite the biotrophic nature of the interaction, this included the non-canonical activation of a jasmonic acid/ethylene-dependent transcriptional program. In contrast to a non-adapted powdery mildew pathogen, the adapted powdery mildew fungus is able to defeat the accumulation of defense-relevant indolic metabolites in a MLO protein-dependent manner. We suggest that a broad and fast activation of immune responses in mlo2 mlo6 mlo12 plants can compensate for the lack of single or few defense pathways. In addition, our results point to a role of Arabidopsis MLO2, MLO6, and MLO12 in enabling defense suppression during invasion by adapted powdery mildew fungi.
The Arabidopsis thaliana lectin receptor kinase LecRK-I.9 is required for full resistance to Pseudomonas syringae and affects jasmonate signalling
Balagué, Claudine ; Gouget, Anne ; Bouchez, Olivier ; Souriac, Camille ; Haget, Nathalie ; Boutet-Mercey, Stéphanie ; Govers, Francine ; Roby, Dominique ; Canut, Hervé - \ 2016
Molecular Plant Pathology 18 (2016)7. - ISSN 1464-6722 - p. 937 - 948.
Arabidopsis - Jasmonic acid - Lectin - Plant-pathogen interactions - Pseudomonas - Receptor-like kinases
On microbial attack, plants can detect invaders and activate plant innate immunity. For the detection of pathogen molecules or cell wall damage, plants employ receptors that trigger the activation of defence responses. Cell surface proteins that belong to large families of lectin receptor kinases are candidates to function as immune receptors. Here, the function of LecRK-I.9 (At5g60300), a legume-type lectin receptor kinase involved in cell wall-plasma membrane contacts and in extracellular ATP (eATP) perception, was studied through biochemical, gene expression and reverse genetics approaches. In Arabidopsis thaliana, LecRK-I.9 expression is rapidly, highly and locally induced on inoculation with avirulent strains of Pseudomonas syringae pv. tomato (Pst). Two allelic lecrk-I.9 knock-out mutants showed decreased resistance to Pst. Conversely, over-expression of LecRK-I.9 led to increased resistance to Pst. The analysis of defence gene expression suggests an alteration of both the salicylic acid (SA) and jasmonic acid (JA) signalling pathways. In particular, LecRK-I.9 expression during plant-pathogen interaction was dependent on COI1 (CORONATINE INSENSITIVE 1) and JAR1 (JASMONATE RESISTANT 1) components, and JA-responsive transcription factors (TFs) showed altered levels of expression in plants over-expressing LecRK-I.9. A similar misregulation of these TFs was obtained by JA treatment. This study identified LecRK-I.9 as necessary for full resistance to Pst and demonstrated its involvement in the control of defence against pathogens through a regulation of JA signalling components. The role of LecRK-I.9 is discussed with regard to the potential molecular mechanisms linking JA signalling to cell wall damage and/or eATP perception.