The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis
Wijnker, Erik ; Harashima, Hirofumi ; Müller, Katja ; Parra-Nuñez, Pablo ; Bastiaan de Snoo, C. ; Belt, Jose van de; Dissmeyer, Nico ; Bayer, Martin ; Pradillo, Monica ; Schnittger, Arp - \ 2019
Proceedings of the National Academy of Sciences of the United States of America 116 (2019)25. - ISSN 0027-8424 - p. 12534 - 12539.
Class - Cross-over interference - Crossovers - Cyclin-dependent kinase - Meiosis - Meiotic recombination
Little is known how patterns of cross-over (CO) numbers and distribution during meiosis are established. Here, we reveal that cyclin-dependent kinase A;1 (CDKA;1), the homolog of human Cdk1 and Cdk2, is a major regulator of meiotic recombination in Arabidopsis. Arabidopsis plants with reduced CDKA;1 activity experienced a decrease of class I COs, especially lowering recombination rates in centromere-proximal regions. Interestingly, this reduction of type I CO did not affect CO assurance, a mechanism by which each chromosome receives at least one CO, resulting in all chromosomes exhibiting similar genetic lengths in weak loss-of-function cdka;1 mutants. Conversely, an increase of CDKA;1 activity resulted in elevated recombination frequencies. Thus, modulation of CDKA;1 kinase activity affects the number and placement of COs along the chromosome axis in a dose-dependent manner.