Phylogeny and genetic diversity of the banana Fusarium wilt pathogen Fusarium oxysporum f. sp. cubense in the Indonesian centre of origin
Maryani, N. ; Lombard, L. ; Poerba, Y.S. ; Subandiyah, S. ; Crous, P.W. ; Kema, G.H.J. - \ 2019
Studies in Mycology 92 (2019). - ISSN 0166-0616 - p. 155 - 194.
11 New taxa - Morphology - New species - Panama disease - Pathogenicity - Tropical Race 4
Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt or Panama disease on banana, is one of the major constraints in banana production worldwide. Indonesia is the centre of origin for wild and cultivated bananas, which likely co-evolved with Foc. This study explored the widest possible genetic diversity of Foc by sampling across Indonesia at 34 geographically and environmentally different locations in 15 provinces at six islands. This resulted in a comprehensive collection of ∼200 isolates from 40 different local banana varieties. Isolates were identified and assessed using sequence analysis of the translation elongation factor-1alpha (tef1), the RNA polymerase II largest subunit (rpb1), and the RNA polymerase II second largest subunit (rpb2). Phylogenetic analyses of these genes allowed the identification of 180 isolates of Fusarium oxysporum f. sp. cubense (Foc), and 20 isolates of the Fusarium fujikuroi species complex (FFSC), the Fusarium incarnatum-equiseti species complex (FIESC), and the Fusarium sambucinum species complex (FSSC). Further analyses, incorporating a worldwide collection of Foc strains, revealed nine independent genetic lineages for Foc, and one novel clade in the Fusarium oxysporum species complex (FOSC). Selected isolates from each lineage were tested on the banana varieties Gros Michel and Cavendish to characterise their pathogenicity profiles. More than 65 % of the isolates were diagnosed as Tropical Race 4 (Foc-TR4) due to their pathogenicity to Cavendish banana, which supports the hypothesis that Foc-TR4 is of Indonesian origin. Nine independent genetic lineages for Foc are formally described in this study. This biodiversity has not been studied since the initial description of Foc in 1919. This study provides a detailed overview of the complexity of Fusarium wilt on banana and its diversity and distribution across Indonesia.
Multi-locus phylogeny and taxonomy of exserohilum
Hernández-Restrepo, M. ; Madrid, H. ; Tan, Y.P. ; Cunha, K.C. da; Gené, J. ; Guarro, J. ; Crous, P.W. - \ 2018
Persoonia 41 (2018). - ISSN 0031-5850 - p. 71 - 108.
Curvularia - Helminthosporium - Human and plant pathogen - New species - Setosphaeria - Systematics
Exserohilum includes a number of plant pathogenic, saprobic and clinically relevant fungi. Some of these species are of great importance in human activities, but the genus has never been revised in a phylogenetic framework. In this study, we revise Exserohilum based on available ex-type cultures from worldwide collections, observation of the holotypes and/or protologues, and additional isolates from diverse substrates and geographical origins. Based on nine nuclear loci, i.e., ITS, LSU, act, tub2, cam, gapdh, his, tef1 and rpb2, as well as phenotypic data, the genus and species boundaries are assessed for Exserohilum. Three species, i.e., E. novae-zelandiae, E. paspali and E. sorghicola, are excluded from the genus and reallocated in Sporidesmiella and Curvularia, respectively, whereas E. heteropogonicola and E. inaequale are confirmed as members of Curvularia. Exserohilum rostratum is revealed as conspecific with species previously described in Exserohilum such as E. antillanum, E. gedarefense, E. leptochloae, E. longirostratum, E. macginnisii and E. prolatum. Additionally, E. curvatum is revealed as synonym of E. holmii, and E. fusiforme of E. oryzicola. A total of 11 Exserohilum phylogenetic species are described, illustrated and discussed, including one novel taxon, E. corniculatum. The placements of 15 other doubtful species are discussed, and E. elongatum is validated.
On a collection of deep-water shrimp (Crustacea, Decapoda) from the Dutch Caribbean, with the description of a new species of Pseudocoutierea
Olthof, Gabriël ; Becking, Leontine E. ; Fransen, Charles H.J.M. - \ 2018
Zootaxa 4415 (2018)3. - ISSN 1175-5326 - p. 533 - 548.
Crustacea - Decapoda - Deep reef - Dutch Caribbean - New records - New species - Pseudocoutierea
A collection of shrimp from deep reefs in the Dutch Caribbean is described. Most material originates from the Bonaire deep reef expedition (2013) by Wageningen Marine Research of Wageningen University. Some additional material was available from dives on Curaçao (2014). A new species of Pseudocoutierea Holthuis was recognized in the material collected off Bonaire. The new species is described and illustrated and its position in the phylogeny of the genus Pseudocoutierea analyzed. A key to the species in the genus is presented.
Senecio canoi (Compositae), Una especie nueva de los Andes de Perú
Montesinos, Daniel ; Gonzáles, Paúl ; Navarro, Eduardo - \ 2015
Anales del Jardín Botánico de Madrid 72 (2015)2. - ISSN 0211-1322
Asteraceae - New species - Peru - Puno - Senecio
Senecio canoi, a new species of Compositae of the high Andes of Southern Peru, Puno department, is described and illustrated. Senecio canoi is a perennial species differing from others in the ser. Suffruticosi subser. Caespitosi by having tufted habit, oblong-spathulate leaves with toothed margin, glabrous, and white flowers.