|An automated modular microsystem for enzymatic digestion with gut-on-a-chip applications
Haan, P. de; Ianovska, M.A. ; Mathwig, K. ; Bouwmeester, H. ; Verpoorte, E. - \ 2020
In: 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. - Chemical and Biological Microsystems Society (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ) - ISBN 9780692941836 - p. 1593 - 1594.
Digestion - Enzyme kinetics - Gut-on-a-chip - Organ-on-a-chip
Gut-on-a-chip models have gained attention as replacements for other cell-based assays or animal studies in drug development or toxicological studies. These models aim to provide a more accurate representation of the in vivo situation in form and function; however, no digestive processes have been included in these systems so far. This work describes a miniaturized digestive system based on artificial digestive juices that digest liquid samples in a series of three microreactors. After optimization of the pH value of juices and mixtures, samples leading to fluorescent products were digested to demonstrate enzyme functionality and to determine kinetic parameters.
Online and in situ analysis of organs-on-a-chip
Santbergen, Milou J.C. ; Zande, Meike van der; Bouwmeester, Hans ; Nielen, Michel W.F. - \ 2019
TrAC : Trends in Analytical Chemistry 115 (2019). - ISSN 0165-9936 - p. 138 - 146.
Electrochemical sensor - Mass spectrometry - Online analysis - Optical detector - Organ-on-a-chip
Organ-on-a-chip technology is used to study biological processes that involve multiple cell types and temporal changes like, homeostasis, metabolism and responses to chemical triggers. Main benefits of organ-on-a-chip systems include: improved mimicking of the in vivo situation, easy manipulation of the microenvironment and low reagent consumption. Exploiting the unique dynamic aspects of organ-on-a-chip technology, such as liquid flow, automated online measurement of parameters by sensors or online coupling to analytical equipment becomes feasible. Apart from the challenge to detect drug uptake and chemical changes in real-time with high resolution at the microscale, the biggest challenge, is detection of the analyte of interest in cell culture medium, as this contains high amounts of salts, sugars and proteins required by the living cells. In this review online and in situ analytical techniques integrated with organ-on-a-chip devices are discussed with special emphasis on maintaining the biological relevance, achieving analytical compatibility, system integration and final applicability.