Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    We will mail you new results for this query: keywords==Sucrose
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High light accelerates potato flowering independently of the FT-like flowering signal StSP3D
Plantenga, Faline D.M. ; Bergonzi, Sara ; Bachem, Christian W.B. ; Visser, Richard G.F. ; Heuvelink, Ep ; Marcelis, Leo F.M. - \ 2019
Environmental and Experimental Botany 160 (2019). - ISSN 0098-8472 - p. 35 - 44.
Daily light integral (DLI) - Flowering - Potato - StSP3D - StTPS1 - Sucrose

Little is known on the environmental control of potato flowering. With recent developments in potato breeding, and the growing interest in true potato seed production, more knowledge on potato flowering is required. This research aims to elucidate the effect of the daily light integral (DLI: the accumulated light throughout a day) on potato flower initiation time and investigate which mechanisms underlie this control. We grew potato plants in climate chambers to compare flower initiation under different DLIs in short and long days. We measured the time until the first appearance of the flower buds and the number of leaves formed before the inflorescence. Furthermore, gene expression changes of the potato flowering time gene StSP3D were measured, and potato plants silenced in StSP3D were used to determine whether DLI mediated flowering time was regulated through StSP3D. Additionally, we determined sucrose and starch concentrations and measured the transcription levels of StTPS1, a gene involved in sugar mediated flowering control. Increasing DLI clearly accelerated flowering in potato. The role of carbohydrates (sucrose and starch) and StTPS1 in DLI-accelerated flowering was inconclusive. Although StSP3D was upregulated under high DLI, transgenic lines silenced in StSP3D also showed accelerated flowering under higher DLIs. We therefore conclude that high DLI accelerates potato flowering and this acceleration happens independently of StSP3D upregulation.

Increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis
Ribeiro, Rafael V. ; Machado, Eduardo C. ; Magalhães Filho, José R. ; Lobo, Ana K.M. ; Martins, Márcio O. ; Silveira, Joaquim A.G. ; Yin, Xinyou ; Struik, Paul C. - \ 2017
Journal of Plant Physiology 208 (2017). - ISSN 0176-1617 - p. 61 - 69.
Photosynthesis - Saccharum - Source-sink - Sucrose - Sugarcane

Spraying sucrose inhibits photosynthesis by impairing Rubisco activity and stomatal conductance (gs), whereas increasing sink demand by partially darkening the plant stimulates sugarcane photosynthesis. We hypothesized that the stimulatory effect of darkness can offset the inhibitory effect of exogenous sucrose on photosynthesis. Source-sink relationship was perturbed in two sugarcane cultivars by imposing partial darkness, spraying a sucrose solution (50 mM) and their combination. Five days after the onset of the treatments, the maximum Rubisco carboxylation rate (Vcmax) and the initial slope of A-Ci curve (k) were estimated by measuring leaf gas exchange and chlorophyll fluorescence. Photosynthesis was inhibited by sucrose spraying in both genotypes, through decreases in Vcmax, k, gs and ATP production driven by electron transport (Jatp). Photosynthesis of plants subjected to the combination of partial darkness and sucrose spraying was similar to photosynthesis of reference plants for both genotypes. Significant increases in Vcmax, gs and Jatp and marginal increases in k were noticed when combining partial darkness and sucrose spraying compared with sucrose spraying alone. Our data also revealed that increases in sink strength due to partial darkness offset the inhibition of sugarcane photosynthesis caused by sucrose spraying, enhancing the knowledge on endogenous regulation of sugarcane photosynthesis through the source-sink relationship.

Osmolality and non-structural carbohydrate composition in the secondary phloem of trees across a latitudinal gradient in Europe
Lintunen, Anna ; Paljakka, Teemu ; Jyske, Tuula ; Peltoniemi, Mikko ; Sterck, Frank ; Arx, Georg Von; Cochard, Hervé ; Copini, Paul ; Caldeira, Maria C. ; Delzon, Sylvain ; Gebauer, Roman ; Grönlund, Leila ; Kiorapostolou, Natasa ; Lechthaler, Silvia ; Lobo-Do-Vale, Raquel ; Peters, Richard L. ; Petit, Giai ; Prendin, Angela L. ; Salmon, Yann ; Steppe, Kathy ; Urban, Josef ; Juan, Sílvia Roig ; Robert, Elisabeth M.R. ; Hölttä, Teemu - \ 2016
Frontiers in Plant Science 7 (2016)JUNE2016. - ISSN 1664-462X
Hexose - Osmotic concentration - Phloem water content - Pinitol - Raffinose - Starch - Sucrose

Phloem osmolality and its components are involved in basic cell metabolism, cell growth, and in various physiological processes including the ability of living cells to withstand drought and frost. Osmolality and sugar composition responses to environmental stresses have been extensively studied for leaves, but less for the secondary phloem of plant stems and branches. Leaf osmotic concentration and the share of pinitol and raffinose among soluble sugars increase with increasing drought or cold stress, and osmotic concentration is adjusted with osmoregulation. We hypothesize that similar responses occur in the secondary phloem of branches. We collected living bark samples from branches of adult Pinus sylvestris, Picea abies, Betula pendula and Populus tremula trees across Europe, from boreal Northern Finland to Mediterranean Portugal. In all studied species, the observed variation in phloem osmolality was mainly driven by variation in phloem water content, while tissue solute content was rather constant across regions. Osmoregulation, in which osmolality is controlled by variable tissue solute content, was stronger for Betula and Populus in comparison to the evergreen conifers. Osmolality was lowest in mid-latitude region, and from there increased by 37% toward northern Europe and 38% toward southern Europe due to low phloem water content in these regions. The ratio of raffinose to all soluble sugars was negligible at mid-latitudes and increased toward north and south, reflecting its role in cold and drought tolerance. For pinitol, another sugar known for contributing to stress tolerance, no such latitudinal pattern was observed. The proportion of sucrose was remarkably low and that of hexoses (i.e., glucose and fructose) high at mid-latitudes. The ratio of starch to all non-structural carbohydrates increased toward the northern latitudes in agreement with the build-up of osmotically inactive C reservoir that can be converted into soluble sugars during winter acclimation in these cold regions. Present results for the secondary phloem of trees suggest that adjustment with tissue water content plays an important role in osmolality dynamics. Furthermore, trees acclimated to dry and cold climate showed high phloem osmolality and raffinose proportion.

Butanol Production from Leftover Beverages and Sport Drinks
Raganati, Francesca ; Procentese, Alessandra ; Montagnaro, Fabio ; Olivieri, Giuseppe ; Marzocchella, Antonio - \ 2015
Bio Energy Research 8 (2015)1. - ISSN 1939-1234 - p. 369 - 379.
Butanol - Clostridium acetobutylicum - Fructose - High-sugar-content beverage - Sucrose

The aim of this paper is twofold: (1) to identify an alternative disposal process for the industry of high-sugar-content beverages (HSCBs) and (2) to contribute to the study of butanol production from non-edible feedstocks. HSCBs were used as a renewable feedstock to produce butanol by Clostridium acetobutylicum DSM 792. Four types of commercial beverages were investigated: fruit juices (pineapple and pear), syrups (lemon and almond), soft drinks, and a sport drink. They contained high levels of sugar—between 50 and 1,000 g/L—mainly glucose, fructose, and sucrose. These sugars were also tested individually and in a mixture. The batch fermentation tests were characterized in terms of metabolite and cell production, and sugar conversion. C. acetobutylicum was able to grow on HSCBs supplemented with yeast extract and minerals. The tests on the sugars (alone or mixed) highlighted the predisposition of C. acetobutylicum to metabolize them and to produce butanol. The production rate of butanol (rB MAX) increased in the following order: glucose, fructose, and sucrose. The HSCB pre-hydrolysis improved the fermentation performance in terms of solvent production and sugar conversion (almost 100 %) because sucrose has been hydrolyzed. Under optimized conditions the butanol concentration and yield were—13.3 g/L, 0.16 g/g for pineapple juice; 12.8 g/L, 0.14 g/g for pear juice; 13.2 g/L, 0.19 g/g for lemon syrup; 13.8 g/L, 0.14 g/g for almond syrup; 13–14 g/L, 0.17–0.18 g/g for commercial soft drinks, and 5.9 g/L, 0.11 g/g for sport drinks. Among the beverages tested, the sport drinks gave the maximum butanol production rate of 12 g/L h.

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